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Peer-reviewedCC BYPhysiological reports

Expression of the long noncoding RNA Neat1 in saliva, blood, and ventromedial hypothalamus of pigs exposed to psychosocial stressors.

  • Dane Webster1
  • Luoyang Ding2
  • Song Zhang1
  • Darryl D'Souza3
  • Alan Tilbrook4
  • Katelyn Tomas4
  • Sarah Babington2
  • Kate Plush3
  • Dominique Blache2
  • Archa Fox1
4 affiliations
  1. 1School of Human Sciences, The University of Western Australia, Crawley, Western Australia, Australia.
  2. 2UWA School of Agriculture and Environment, The University of Western Australia, Crawley, Western Australia, Australia.
  3. 3SunPork Group, Eagle Farm, Queensland, Australia.
  4. 4School of Veterinary Science, The University of Queensland, Brisbane, Queensland, Australia.
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Abstract

The long noncoding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) is a master regulator of transcriptional and posttranscriptional changes in stress responses. Here we assessed Neat1 lncRNA expression using quantitative PCR in peripheral biological fluids (saliva and blood) and a brain region that regulates stress and emotion (ventromedial hypothalamus) of 12-week-old pigs exposed to chronic (social mixing causing conflict) and acute (restraint) psychosocial challenges. We demonstrated that Neat1 is measurable in pig saliva, blood, and brain samples. Successful Neat1 lncRNA measurements in all three tissue types further established both broad tissue expression as well as the cross-species conserved sequence of the NEAT1 gene. We found that Neat1_2 and Neat1 total expression did not correlate between tissues, suggesting variable expression throughout the body, and no significant differences in Neat1 lncRNA levels with either stressor. We also observed across all treatment groups changes in Neat1_2 and cortisol levels comparing samples taken before the intervention, compared to after, indicating confounding factors may have impacted the model. Overall, this study demonstrates the feasibility of measuring Neat1 in biological fluids obtained from farm mammals, with further work required to assess Neat1 lncRNA as a molecule involved in the physiological response to psychosocial stress.

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Cite

BibTeXRIS
BibTeX
@article{webster2026expression,
  title = {{Expression of the long noncoding RNA Neat1 in saliva, blood, and ventromedial hypothalamus of pigs exposed to psychosocial stressors.}},
  author = {Dane Webster and Luoyang Ding and Song Zhang and Darryl D'Souza and Alan Tilbrook and Katelyn Tomas and Sarah Babington and Kate Plush and Dominique Blache and Archa Fox},
  journal = {Physiological reports},
  year = {2026},
  month = oct,
  doi = {10.14814/phy2.71130},
  url = {https://europepmc.org/article/MED/42897969},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Expression of the long noncoding RNA Neat1 in saliva, blood, and ventromedial hypothalamus of pigs exposed to psychosocial stressors.
AU  - Dane Webster
AU  - Luoyang Ding
AU  - Song Zhang
AU  - Darryl D'Souza
AU  - Alan Tilbrook
AU  - Katelyn Tomas
AU  - Sarah Babington
AU  - Kate Plush
AU  - Dominique Blache
AU  - Archa Fox
PY  - 2026
DA  - 2026/10/01
JO  - Physiological reports
DO  - 10.14814/phy2.71130
UR  - https://europepmc.org/article/MED/42897969
AB  - The long noncoding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) is a master regulator of transcriptional and posttranscriptional changes in stress responses. Here we assessed Neat1 lncRNA expression using quantitative PCR in peripheral biological fluids (saliva and blood) and a brain region that regulates stress and emotion (ventromedial hypothalamus) of 12-week-old pigs exposed to chronic (social mixing causing conflict) and acute (restraint) psychosocial challenges. We demonstrated that Neat1 is measurable in pig saliva, blood, and brain samples. Successful Neat1 lncRNA measurements in all three tissue types further established both broad tissue expression as well as the cross-species conserved sequence of the NEAT1 gene. We found that Neat1_2 and Neat1 total expression did not correlate between tissues, suggesting variable expression throughout the body, and no significant differences in Neat1 lncRNA levels with either stressor. We also observed across all treatment groups changes in Neat1_2 and cortisol levels comparing samples taken before the intervention, compared to after, indicating confounding factors may have impacted the model. Overall, this study demonstrates the feasibility of measuring Neat1 in biological fluids obtained from farm mammals, with further work required to assess Neat1 lncRNA as a molecule involved in the physiological response to psychosocial stress.
SN  - 2051-817X
ER  -