Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage
Abstract
This study investigated the dose-dependent effects of a lactobacilli-based postbiotic ( Post ) on the transcriptional reprogramming of the chicken macrophage-like HD11 cell line when exposed to Escherichia coli lipopolysaccharide ( LPS ). First, the HD11cells were treated with 0, 3, 30 and 300 ng/mL LPS in combination with 0, 0.2, 0.4, 0.6 and 0.8% v/v Post. Nitric oxide ( NO ) production was quantified at 20 h incubation and the early transcriptome reprogramming was analysed in a subset of treatments at 5 h incubation. Results Post increased NO production dose-dependently and an LPS-postbiotic interaction was present, with the cells eliciting a higher NO production in response to Post at 30 and 300 ng/ml LPS compared to the zero and 3 ng/ml LPS. To further understand this interaction, the early transcriptome reprogramming was investigated for treatments with 0, 3 and 300 ng/mL LPS and 0 and 0.8% v/v Post. A number of differentially expressed genes were identified and gene set enrichment analysis of KEGG pathways revealed that Post at 0 and 300 ng/mL LPS influenced similar inflammation-related pathways, until Post at 3 ng/mL LPS which had a minimal effect. Expression of transcription factors ( TFs ) and immune-related genes revealed differential effects induced by Post depending on LPS concentration which would have likely influenced the inflammatory response. Specifically, the only TFs affected by Post at 300 ng/ml LPS were STAT2 , SMAD3 and IFR8 , which all showed increased expression. The TFs consistently affected by Post at the zero and 3 ng/ml LPS increased and were JUN , ZFP36L2 , SMAD1 and E2F3 . Conclusion Our results showed that Post had a pro-inflammatory effect, which was exacerbated in the presence of a 300 but not 3 ng/ml LPS. Furthermore, the dose of LPS affected the sensitivity of the cells to Post. Dose-response studies should be performed when investigating the effects of dietary compounds on inflammation in chicken macrophages.
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BibTeX
@article{jansseune2026lactobacillibased,
title = {{Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage}},
author = {Samuel Jansseune and Jürgen van Baal and Fany Blanc and Aart Lammers},
journal = {BMC Veterinary Research},
year = {2026},
month = oct,
doi = {10.1186/s12917-025-04902-w},
url = {https://hal.inrae.fr/hal-05447387v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage
AU - Samuel Jansseune
AU - Jürgen van Baal
AU - Fany Blanc
AU - Aart Lammers
PY - 2026
DA - 2026/10/29
JO - BMC Veterinary Research
DO - 10.1186/s12917-025-04902-w
UR - https://hal.inrae.fr/hal-05447387v1
AB - This study investigated the dose-dependent effects of a lactobacilli-based postbiotic ( Post ) on the transcriptional reprogramming of the chicken macrophage-like HD11 cell line when exposed to Escherichia coli lipopolysaccharide ( LPS ). First, the HD11cells were treated with 0, 3, 30 and 300 ng/mL LPS in combination with 0, 0.2, 0.4, 0.6 and 0.8% v/v Post. Nitric oxide ( NO ) production was quantified at 20 h incubation and the early transcriptome reprogramming was analysed in a subset of treatments at 5 h incubation. Results Post increased NO production dose-dependently and an LPS-postbiotic interaction was present, with the cells eliciting a higher NO production in response to Post at 30 and 300 ng/ml LPS compared to the zero and 3 ng/ml LPS. To further understand this interaction, the early transcriptome reprogramming was investigated for treatments with 0, 3 and 300 ng/mL LPS and 0 and 0.8% v/v Post. A number of differentially expressed genes were identified and gene set enrichment analysis of KEGG pathways revealed that Post at 0 and 300 ng/mL LPS influenced similar inflammation-related pathways, until Post at 3 ng/mL LPS which had a minimal effect. Expression of transcription factors ( TFs ) and immune-related genes revealed differential effects induced by Post depending on LPS concentration which would have likely influenced the inflammatory response. Specifically, the only TFs affected by Post at 300 ng/ml LPS were STAT2 , SMAD3 and IFR8 , which all showed increased expression. The TFs consistently affected by Post at the zero and 3 ng/ml LPS increased and were JUN , ZFP36L2 , SMAD1 and E2F3 . Conclusion Our results showed that Post had a pro-inflammatory effect, which was exacerbated in the presence of a 300 but not 3 ng/ml LPS. Furthermore, the dose of LPS affected the sensitivity of the cells to Post. Dose-response studies should be performed when investigating the effects of dietary compounds on inflammation in chicken macrophages.
SN - 1746-6148
ER -