Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 4 min ago
Peer-reviewedCC BYScientific Reports

GaRD21A, a cysteine protease gene in Gossypium arboreum is associated with defense response against cotton leaf curl disease

  • Muhammad Qasim Aslam1
  • Athar Hussain2
  • Rida Zahid1
  • Afzal Akram1
  • Khalid Pervaiz Akhtar3
  • Rubab Zahra Naqvi1
  • Imran Amin1
  • Shahid Mansoor1
  • Muhammad Saeed1
  1. 1National Institute for Biotechnology and Genetic Engineering, College of Pakistan Institute of Engineering and Applied Sciences (PIEAS)
  2. 2Department of Biochemistry and Biotechnology, University of Gujrat
  3. 3Nuclear Institute for Agriculture & Biology, College of Pakistan Institute of Engineering and Applied Sciences (PIEAS)
Read full textPDFDOICite

Abstract

Cysteine proteases (CPs) of the C1-papain family play important roles in plant immunity, particularly in antiviral and antifungal defenses. However, this gene family has not yet been characterized in cotton under Cotton Leaf Curl Disease (CLCuD) stress. Genome-wide diversity analysis and functional annotation revealed the abundance, evolutionary conservation, and conserved peptidase signatures of CP family members across ten Gossypium species and an ancestrally related species Theobroma cacao. Furthermore, a virus-induced gene silencing (VIGS) assay was performed to investigate the role of a papain-like CP gene GaRD21A in resistance response of Gossypium arboreum against CLCuD. Silencing of GaRD21A resulted in increased virus accumulation in both viruliferous whitefly-exposed and graft-mediated CLCuD inoculated plants; however, pronounced vein thickening symptoms were only observed in graft-inoculated plants. To the best of our knowledge, this is the first report demonstrating the involvement of a CP family gene in the immune response of G. arboreum against CLCuD, providing a potential target for cotton resistance breeding programs.

Related

  1. ERK-mediated phosphorylation modulates the autolytic processing of the cysteine protease CAPN3
    Chihiro Hisatsune, Fumiko Shinkai-Ouchi, Mayumi Shindo +2
    Journal of Biological ChemistryPeer-reviewedCC BY 4.0Cell biologyCrossref
  2. Arabidopsis cysteine protease RD19 limits RBOHD-generated ROS production in pattern triggered immunity
    G. Hessler, M. Mantz, F. Locci +3
    bioRxivPreprintCC BY-NC-NDFull textPlant biologybioRxiv
  3. Protease-sensitive antibacterial activity from tilapia gut-derived Enterobacter ludwigii is associated with a colicin V-like genomic candidate
    Arnab Chatterjee, Krishnendu Sinha, Kausik Mondal +1
    Scientific ReportsPeer-reviewedCC BY-NC-ND 4.0Full textImmunologyCrossref
  4. Phenotypic and genetic variation among Rhododendron arboreum species complex widely distributed throughout the Himalayas suggests an obscure geographic origin of the clade
    Shuichi Noshiro, Hiroshi Ikeda, Diego Tavares Vasques +5
    Annals of BotanyPeer-reviewedPublisher termsFull textEvolutionary biologyPMC
  5. Beyond elevation: climate, geography, and the diversification of Himalayan Rhododendrons. A commentary on ‘Phenotypic and genetic variation among Rhododendron arboreum species complex widely distributed throughout the Himalayas suggests an obscure geographic origin of the clade’
    Dinesh Thakur, Manish Kumar Sharma, Nikita Rathore
    Annals of BotanyPeer-reviewedPublisher termsFull textEvolutionary biologyPMC

Cite

BibTeXRIS
BibTeX
@article{aslam2026gard21a,
  title = {{GaRD21A, a cysteine protease gene in Gossypium arboreum is associated with defense response against cotton leaf curl disease}},
  author = {Muhammad Qasim Aslam and Athar Hussain and Rida Zahid and Afzal Akram and Khalid Pervaiz Akhtar and Rubab Zahra Naqvi and Imran Amin and Shahid Mansoor and Muhammad Saeed},
  journal = {Scientific Reports},
  year = {2026},
  month = aug,
  doi = {10.1038/s41598-026-64111-9},
  url = {https://doi.org/10.1038/s41598-026-64111-9},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - GaRD21A, a cysteine protease gene in Gossypium arboreum is associated with defense response against cotton leaf curl disease
AU  - Muhammad Qasim Aslam
AU  - Athar Hussain
AU  - Rida Zahid
AU  - Afzal Akram
AU  - Khalid Pervaiz Akhtar
AU  - Rubab Zahra Naqvi
AU  - Imran Amin
AU  - Shahid Mansoor
AU  - Muhammad Saeed
PY  - 2026
DA  - 2026/08/01
JO  - Scientific Reports
DO  - 10.1038/s41598-026-64111-9
UR  - https://doi.org/10.1038/s41598-026-64111-9
AB  - Cysteine proteases (CPs) of the C1-papain family play important roles in plant immunity, particularly in antiviral and antifungal defenses. However, this gene family has not yet been characterized in cotton under Cotton Leaf Curl Disease (CLCuD) stress. Genome-wide diversity analysis and functional annotation revealed the abundance, evolutionary conservation, and conserved peptidase signatures of CP family members across ten Gossypium species and an ancestrally related species Theobroma cacao. Furthermore, a virus-induced gene silencing (VIGS) assay was performed to investigate the role of a papain-like CP gene GaRD21A in resistance response of Gossypium arboreum against CLCuD. Silencing of GaRD21A resulted in increased virus accumulation in both viruliferous whitefly-exposed and graft-mediated CLCuD inoculated plants; however, pronounced vein thickening symptoms were only observed in graft-inoculated plants. To the best of our knowledge, this is the first report demonstrating the involvement of a CP family gene in the immune response of G. arboreum against CLCuD, providing a potential target for cotton resistance breeding programs.
SN  - 2045-2322
ER  -