Fluorescent PyrAte-( S )-citalopram conjugates enable imaging of the serotonin transporter in living tissue

Abstract

imaging of endogenous SERT was demonstrated in acute mouse brain slices using two-photon microscopy. The large Stokes shift of the PyrAte fluorophore enables simultaneous detection of its own fluorescence signal at 500 nm along with that of a yellow fluorescent protein-based serotonergic marker. Our findings provide novel tools for unprecedented SERT visualization and establish the utility of PyrAtes for the selective staining of membrane proteins in live cells and tissue.

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BibTeX RIS

BibTeX

@article{belleza2026fluorescent,
  title = {{Fluorescent PyrAte-( S )-citalopram conjugates enable imaging of the serotonin transporter in living tissue}},
  author = {Oliver Belleza and Iakovos Saridakis and Nadja Singer and Xavier Westergaard and Sergio Armentia Matheu and Miran Lemmerer and Margaux Riomet and Pedro Sánchez-Murcia and Nina Kastner and Stefanie Rukavina and Yi Xiao and Kathrin Jäntsch and Marco Niello and Klaus Schicker and David Sulzer and Leticia González and Nuno Maulide and Harald Sitte},
  journal = {Chemical Science},
  year = {2026},
  month = sep,
  doi = {10.1039/d4sc06949h},
  url = {https://hal.science/hal-05751970v1},
  note = {CC BY},
}

RIS

TY  - JOUR
TI  - Fluorescent PyrAte-( S )-citalopram conjugates enable imaging of the serotonin transporter in living tissue
AU  - Oliver Belleza
AU  - Iakovos Saridakis
AU  - Nadja Singer
AU  - Xavier Westergaard
AU  - Sergio Armentia Matheu
AU  - Miran Lemmerer
AU  - Margaux Riomet
AU  - Pedro Sánchez-Murcia
AU  - Nina Kastner
AU  - Stefanie Rukavina
AU  - Yi Xiao
AU  - Kathrin Jäntsch
AU  - Marco Niello
AU  - Klaus Schicker
AU  - David Sulzer
AU  - Leticia González
AU  - Nuno Maulide
AU  - Harald Sitte
PY  - 2026
DA  - 2026/09/16
JO  - Chemical Science
DO  - 10.1039/d4sc06949h
UR  - https://hal.science/hal-05751970v1
AB  - imaging of endogenous SERT was demonstrated in acute mouse brain slices using two-photon microscopy. The large Stokes shift of the PyrAte fluorophore enables simultaneous detection of its own fluorescence signal at 500 nm along with that of a yellow fluorescent protein-based serotonergic marker. Our findings provide novel tools for unprecedented SERT visualization and establish the utility of PyrAtes for the selective staining of membrane proteins in live cells and tissue.
SN  - 2041-6520
SN  - 2041-6539
ER  -

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