The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like
- 1Institute of Biochemistry I, Jena University Hospital - Friedrich Schiller University Jena, Jena, Germany
Abstract
Proper neuromorphogenesis is pivotal for neuronal network formation and brain function. The underlying cell biological tasks are complex, as they include both remodeling of membrane topologies and of cytoskeletal structures. Yet, mechanisms coordinating these two different cell biological functions are largely unknown. Here, we molecularly and functionally characterize ankyrin repeat domain-containing protein 24 (Ankrd24) and demonstrate that this ankyrin-repeat protein is able to interact with and bend membranes and mechanistically and functionally belongs to the recently proposed N-Ank superfamily. Mutational analyses revealed that the N-Ank functions of Ankrd24 are indispensable for Ankrd24’s role in neuromorphogenesis. Ankrd24 furthermore physically cooperated with the F-BAR domain protein syndapin I (PACSIN1) and with Cobl-like (Cobll1), a protein promoting actin filament formation, by ternary complex formation. Both partners were critical for Ankrd24-mediated dendritic arborization. This identified Ankrd24 as critical part of a larger, coordinated effort in inducing new dendritic branches. Our work thereby sheds light on the molecular mechanisms underlying the complex task of proper neuronal arbor development.
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BibTeX
@article{jain2026nank,
title = {{The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like}},
author = {Astha Jain and Maryam Izadi-Seitz and Yuanyuan Ji and Eric Seemann and Britta Qualmann and Michael M. Kessels},
journal = {The Journal of Cell Biology},
year = {2026},
month = oct,
doi = {10.1083/jcb.202509074},
url = {https://doi.org/10.1083/jcb.202509074},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - The N-Ank protein Ankrd24 shapes neurons and couples to syndapin and the F-actin promoter Cobl-like
AU - Astha Jain
AU - Maryam Izadi-Seitz
AU - Yuanyuan Ji
AU - Eric Seemann
AU - Britta Qualmann
AU - Michael M. Kessels
PY - 2026
DA - 2026/10/21
JO - The Journal of Cell Biology
DO - 10.1083/jcb.202509074
UR - https://doi.org/10.1083/jcb.202509074
AB - Proper neuromorphogenesis is pivotal for neuronal network formation and brain function. The underlying cell biological tasks are complex, as they include both remodeling of membrane topologies and of cytoskeletal structures. Yet, mechanisms coordinating these two different cell biological functions are largely unknown. Here, we molecularly and functionally characterize ankyrin repeat domain-containing protein 24 (Ankrd24) and demonstrate that this ankyrin-repeat protein is able to interact with and bend membranes and mechanistically and functionally belongs to the recently proposed N-Ank superfamily. Mutational analyses revealed that the N-Ank functions of Ankrd24 are indispensable for Ankrd24’s role in neuromorphogenesis. Ankrd24 furthermore physically cooperated with the F-BAR domain protein syndapin I (PACSIN1) and with Cobl-like (Cobll1), a protein promoting actin filament formation, by ternary complex formation. Both partners were critical for Ankrd24-mediated dendritic arborization. This identified Ankrd24 as critical part of a larger, coordinated effort in inducing new dendritic branches. Our work thereby sheds light on the molecular mechanisms underlying the complex task of proper neuronal arbor development.
SN - 0021-9525
SN - 1540-8140
ER -