circMARK3 serves as a sponge of miR-205a to promote senescence of follicular granulosa cells in laying hens via regulating LIN9
4 affiliations
- 1State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
- 2Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
- 3Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
- 4Sichuan Jinqin Agricultural and Livestock Technology Co., Ltd., Manyang, Sichuan, 621600, China
Abstract
Ovarian aging is a key factor contributing to the decline in reproductive performance of laying hens during the late laying period. A major contributor to ovarian aging is the accumulation of follicular senescence, and granulosa cell (GC) senescence is the molecular basis of follicular senescence. Circular RNAs (circRNAs) can act as competing endogenous RNAs (ceRNAs) by competitively binding microRNAs (miRNAs) and relieving their repression of downstream target genes. However, whether this ceRNA mechanism is involved in GC senescence in laying hens remains unclear. In earlier sequencing, circMARK3 expression was markedly increased in the ovarian follicles of laying hens during the late stage of egg production. Overexpression and knockdown of circMARK3 in primary GCs, combined with SA-β-gal staining, flow cytometry, and Western blotting, confirmed that circMARK3 promotes GC senescence in hens. Cellular fractionation and fluorescence in situ hybridization (FISH) confirmed that circMARK3 is predominantly localized in the cytoplasm, suggesting that it may function through a ceRNA mechanism. Bioinformatics prediction and dual-luciferase reporter assays indicated that circMARK3 directly binds miR-205a. Further experiments indicated that circMARK3 relieves miR-205a-mediated repression of Lin-9 homolog (LIN9), thereby upregulating LIN9 expression and ultimately promoting GC senescence. Collectively, these findings suggest that the circMARK3/miR-205a/LIN9 axis may be involved in GC senescence and follicular dysfunction in hens, providing a potential target for delaying ovarian aging in laying hens.
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BibTeX
@article{liao2026circmark3,
title = {{circMARK3 serves as a sponge of miR-205a to promote senescence of follicular granulosa cells in laying hens via regulating LIN9}},
author = {Chi-mei Liao and Yi-meng Wei and Jia-lin Xiang and Yi-xuan Wang and Xin-yue Zhao and Yao Zhang and Yu-qi Chen and Hua-dong Yin},
journal = {Poultry Science},
year = {2026},
month = oct,
doi = {10.1016/j.psj.2026.107986},
url = {https://doi.org/10.1016/j.psj.2026.107986},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - circMARK3 serves as a sponge of miR-205a to promote senescence of follicular granulosa cells in laying hens via regulating LIN9
AU - Chi-mei Liao
AU - Yi-meng Wei
AU - Jia-lin Xiang
AU - Yi-xuan Wang
AU - Xin-yue Zhao
AU - Yao Zhang
AU - Yu-qi Chen
AU - Hua-dong Yin
PY - 2026
DA - 2026/10/17
JO - Poultry Science
DO - 10.1016/j.psj.2026.107986
UR - https://doi.org/10.1016/j.psj.2026.107986
AB - Ovarian aging is a key factor contributing to the decline in reproductive performance of laying hens during the late laying period. A major contributor to ovarian aging is the accumulation of follicular senescence, and granulosa cell (GC) senescence is the molecular basis of follicular senescence. Circular RNAs (circRNAs) can act as competing endogenous RNAs (ceRNAs) by competitively binding microRNAs (miRNAs) and relieving their repression of downstream target genes. However, whether this ceRNA mechanism is involved in GC senescence in laying hens remains unclear. In earlier sequencing, circMARK3 expression was markedly increased in the ovarian follicles of laying hens during the late stage of egg production. Overexpression and knockdown of circMARK3 in primary GCs, combined with SA-β-gal staining, flow cytometry, and Western blotting, confirmed that circMARK3 promotes GC senescence in hens. Cellular fractionation and fluorescence in situ hybridization (FISH) confirmed that circMARK3 is predominantly localized in the cytoplasm, suggesting that it may function through a ceRNA mechanism. Bioinformatics prediction and dual-luciferase reporter assays indicated that circMARK3 directly binds miR-205a. Further experiments indicated that circMARK3 relieves miR-205a-mediated repression of Lin-9 homolog (LIN9), thereby upregulating LIN9 expression and ultimately promoting GC senescence. Collectively, these findings suggest that the circMARK3/miR-205a/LIN9 axis may be involved in GC senescence and follicular dysfunction in hens, providing a potential target for delaying ovarian aging in laying hens.
SN - 0032-5791
SN - 1525-3171
ER -