TheCellVision.org repository: expansion with high-content cell imaging projects on eukaryotic intracellular organization and DUB biology
- 1University of Toronto
Abstract
High-content cell imaging approaches enable the systematic characterization of cellular function through the acquisition of multimodal information from large cohorts of live single cells. Yet, due to their scale and complexity, data acquired via such approaches are often challenging to meaningfully share across laboratories and effectively use for independent studies. Since its inception, the main purpose of TheCellVision.org repository has been to fill this gap, providing the research community with access to large-scale, multimodal single-cell datasets, in a structured, intuitive, and user-friendly way. Here, we report on the third major update of TheCellVision.org, which involves the expansion of the repository with the addition of data from two single-cell phenomics projects; the Intracellular Organization Dynamics project, which quantitatively maps changes in the morphology of 21 major subcellular structures in live yeast cells elicited by the systematic inhibition of essential genes, and the DUB Biology project, which describes changes in the concentration and localization of the budding yeast proteome in mutants of key deubiquitination enzymes (DUBs). With these additions, the repository now hosts six complementary high-content imaging projects which collectively explore the dynamics of intracellular organization and the proteome during changes in cell state and in response to environmental and genetic perturbations.
Related
- Concanavalin A as a pan-eukaryotic nuclear envelope marker for expansion microscopy
- Live-cell imaging and ultrastructure expansion microscopy reveal the dynamic intracellular life cycle of Encephalitozoon intestinalis
- Correlation of Live Imaging and Fixed-Expansion Microscopy to study intracellular pathogens
- Cellular organization and phylogeny of Cryptonaiada gen. n. (Cryptonaiadidae fam. n.) shed light on the evolution of intracellular vertebrate parasitism in Apicomplexa
- Genomic surveillance-guided phage repository construction and rational cocktail design for carbapenem-resistant
BibTeX
@misc{masinas2026thecellvisionorg,
title = {{TheCellVision.org repository: expansion with high-content cell imaging projects on eukaryotic intracellular organization and DUB biology}},
author = {M. P. D. Masinas and A. Litsios and M. Usaj and H. Garadi Suresch and C. Boone and B. J. Andrews},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.02.748561},
url = {https://www.biorxiv.org/content/10.64898/2026.09.02.748561v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - TheCellVision.org repository: expansion with high-content cell imaging projects on eukaryotic intracellular organization and DUB biology
AU - M. P. D. Masinas
AU - A. Litsios
AU - M. Usaj
AU - H. Garadi Suresch
AU - C. Boone
AU - B. J. Andrews
PY - 2026
DA - 2026/09/02
PB - bioRxiv
DO - 10.64898/2026.09.02.748561
UR - https://www.biorxiv.org/content/10.64898/2026.09.02.748561v1
AB - High-content cell imaging approaches enable the systematic characterization of cellular function through the acquisition of multimodal information from large cohorts of live single cells. Yet, due to their scale and complexity, data acquired via such approaches are often challenging to meaningfully share across laboratories and effectively use for independent studies. Since its inception, the main purpose of TheCellVision.org repository has been to fill this gap, providing the research community with access to large-scale, multimodal single-cell datasets, in a structured, intuitive, and user-friendly way. Here, we report on the third major update of TheCellVision.org, which involves the expansion of the repository with the addition of data from two single-cell phenomics projects; the Intracellular Organization Dynamics project, which quantitatively maps changes in the morphology of 21 major subcellular structures in live yeast cells elicited by the systematic inhibition of essential genes, and the DUB Biology project, which describes changes in the concentration and localization of the budding yeast proteome in mutants of key deubiquitination enzymes (DUBs). With these additions, the repository now hosts six complementary high-content imaging projects which collectively explore the dynamics of intracellular organization and the proteome during changes in cell state and in response to environmental and genetic perturbations.
ER -