Comparative evaluation of the remineralization potential of chitosan-fluoride-doped mesoporous silica nanoparticles hydrogel and casein phosphopeptide-amorphous calcium phosphate fluoride paste on the enamel of primary teeth: An in vitro study
Abstract
Objectives: To compare the remineralization potential of chitosan-fluoride-doped mesoporous silica nanoparticles (FMSN) hydrogel and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste on artificially demineralized enamel of primary teeth. Materials and Methods: Thirty extracted primary central incisors were randomly allocated into two groups (n = 15). Group CH received Chitosan-FMSN hydrogel, and Group GC received CPP-ACPF paste. The remineralizing agents were applied twice daily for 3 months. Remineralization was assessed using DIAGNOdent®, while elemental composition and surface morphology were evaluated using energy-dispersive X-ray spectroscopy and field emission scanning electron microscopy. Statistical Analysis: Intergroup comparisons were performed using an independent Student’s t-test, and intragroup comparisons were performed using a paired t-test. Statistical significance was set at P < 0.05. Results: The CPP-ACPF group showed a significant increase in calcium content from 55.47% to 67.96% (P = 0.001), whereas the Chitosan-FMSN group showed a decrease from 53.59% to 42.37% (P = 0.095). The Ca/P ratio increased from 1.25 to 2.62 in the CPP-ACPF group and decreased from 1.16 to 1.04 in the Chitosan-FMSN group, with a significant intergroup difference at three months (P = 0.003). DIAGNOdent® values decreased from 10.66 to 2.40 in the CPP-ACPF group and from 10.06 to 4.26 in the Chitosan-FMSN group. FE-SEM demonstrated greater surface mineral coverage in the CPP-ACPF group. Conclusions: Under the conditions of this in vitro study, CPP-ACPF demonstrated greater remineralization-associated changes than Chitosan-FMSN hydrogel in primary enamel.
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BibTeX
@article{suma2026comparative,
title = {{Comparative evaluation of the remineralization potential of chitosan-fluoride-doped mesoporous silica nanoparticles hydrogel and casein phosphopeptide-amorphous calcium phosphate fluoride paste on the enamel of primary teeth: An in vitro study}},
author = {N. K. Suma and M.N.Shwetha and Rajeev J. Mudakavi and B. V. Basavaraj and S. Nikitha},
journal = {Journal of Indian Society of Pedodontics and Preventive Dentistry},
year = {2026},
month = sep,
doi = {10.4103/jisppd.jisppd_343_26},
url = {https://doi.org/10.4103/jisppd.jisppd_343_26},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Comparative evaluation of the remineralization potential of chitosan-fluoride-doped mesoporous silica nanoparticles hydrogel and casein phosphopeptide-amorphous calcium phosphate fluoride paste on the enamel of primary teeth: An in vitro study
AU - N. K. Suma
AU - M.N.Shwetha
AU - Rajeev J. Mudakavi
AU - B. V. Basavaraj
AU - S. Nikitha
PY - 2026
DA - 2026/09/01
JO - Journal of Indian Society of Pedodontics and Preventive Dentistry
DO - 10.4103/jisppd.jisppd_343_26
UR - https://doi.org/10.4103/jisppd.jisppd_343_26
AB - Objectives: To compare the remineralization potential of chitosan-fluoride-doped mesoporous silica nanoparticles (FMSN) hydrogel and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste on artificially demineralized enamel of primary teeth. Materials and Methods: Thirty extracted primary central incisors were randomly allocated into two groups (n = 15). Group CH received Chitosan-FMSN hydrogel, and Group GC received CPP-ACPF paste. The remineralizing agents were applied twice daily for 3 months. Remineralization was assessed using DIAGNOdent®, while elemental composition and surface morphology were evaluated using energy-dispersive X-ray spectroscopy and field emission scanning electron microscopy. Statistical Analysis: Intergroup comparisons were performed using an independent Student’s t-test, and intragroup comparisons were performed using a paired t-test. Statistical significance was set at P < 0.05. Results: The CPP-ACPF group showed a significant increase in calcium content from 55.47% to 67.96% (P = 0.001), whereas the Chitosan-FMSN group showed a decrease from 53.59% to 42.37% (P = 0.095). The Ca/P ratio increased from 1.25 to 2.62 in the CPP-ACPF group and decreased from 1.16 to 1.04 in the Chitosan-FMSN group, with a significant intergroup difference at three months (P = 0.003). DIAGNOdent® values decreased from 10.66 to 2.40 in the CPP-ACPF group and from 10.06 to 4.26 in the Chitosan-FMSN group. FE-SEM demonstrated greater surface mineral coverage in the CPP-ACPF group. Conclusions: Under the conditions of this in vitro study, CPP-ACPF demonstrated greater remineralization-associated changes than Chitosan-FMSN hydrogel in primary enamel.
SN - 0970-4388
SN - 1998-3905
ER -