Skip to content
DraconiticResearch
Search Sources API
197,768 studies31 sourcesUpdated 38 min ago
PreprintCC BYbioRxiv

A unique intestinal epithelial cell that expresses canonical lymphoid genes

  • Swarnabh Bhattacharya1,2
  • Krithika Badarinath1,2
  • Guodong Tie1
  • Pratik N. P. Singh1,2
  • Onur Eskiocak3,4
  • Ruiyang He1
  • Bin Bao5
  • Su-Hyung Lee6
  • Scott Snapper2,5
  • Kun Huang7
  • James R. Goldenring6
  • Semir Beyaz3
1 more authors
  • Ramesh A. Shivdasani1,2,8
8 affiliations
  1. 1Department of Medical Oncology and Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
  2. 2Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
  3. 3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
  4. 4Graduate Program in Genetics, State University of New York, Stony Brook, NY 11794, USA.
  5. 5Division of Gastroenterology, Hepatology, and Nutrition, Boston Children’s Hospital, Boston, MA 02115, USA.
  6. 6Section of Surgical Sciences, Epithelial Biology Center, and Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, and Nashville VA Medical Center, Nashville, TN 37212, USA.
  7. 7Molecular Imaging Core and Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
  8. 8Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
Read full textPDFDOICite

Abstract

Enterocytes and intestinal secretory cells harbor innate immunity. However, certain transcriptional programs -even beyond rearranged T- and B-cell receptor genes- are considered the exclusive purview of adaptive immune cells, including abundant and diverse intestinal intra-epithelial lymphocytes (IELs). Here, we identify and characterize columnar intestinal secretory cells that co-express classic epithelial and lymphoid genes, including Ptprc, Cd3e, Cd8a, and Gzma/b but not T- or B-cell receptors. These previously unrecognized epithelium-derived lymphoid-like cells (EDLCs) differ from any known resident cell type, including IELs or tuft cells. Rare in healthy mouse and human intestines, their numbers increase upon IL-25 stimulation or IL-10 receptor deficiency in vivo and upon IL-13 stimulation in vitro. EDLC chromatin is uniquely accessible at thousands of cis-elements, including lymphoid and epithelial loci. These findings reveal a transcriptionally and epigenetically distinct intestinal epithelial derivative that expresses prominent lymphoid character.

Related

  1. SETD3 ACTIN HISTIDINE METHYLTRANSFERASE REGULATES INTESTINAL EPITHELIAL BARRIER INTEGRITY AND MUCOSAL INFLAMMATION
    P. Manogaran, N. G. Naydenov, S. Lechiga +4
    bioRxivPreprintAll rights reservedFull textCell biologybioRxiv
  2. Campylobacter jejuni Infection Is Associated With Cell-Cycle, Redox, and Metabolic Remodeling of Human Intestinal Epithelial Cells at Single-Cell Resolution
    A. Gao, V. Newhart, S. Kassis +2
    bioRxivPreprintAll rights reservedFull textMicrobiologybioRxiv
  3. Intestinal epithelial Casd1 regulates mucus sialic acid O-acetylation and limits tissue damage from large-intestinal mucosal insults
    Simin Jafaripour, Mackenzie Melvin, Megan Turluk +19
    iSciencePeer-reviewedCC BY-NC-ND 4.0ImmunologyCell PressCrossref
  4. Integrated transcriptomics, metabolomics, and gut microbiome analysis reveals the association between Rnf43 knockdown and intestinal epithelial hyperproliferation in HFD/AOM/DSS treated mice
    Ying Geng, Yifang Li, Keqiu Qu +4
    BMC GenomicsPeer-reviewedCC BY 4.0Genetics & genomicsCrossref
  5. The role of metabolic crosstalk between Intestinal epithelial cells and immune cells in inflammatory bowel disease and targeting strategies
    Heng Li, Beibei Wang, Xianhong Shi +6
    Frontiers in immunologyPeer-reviewedCC BYFull textImmunologyEurope PMC

Cite

BibTeXRIS
BibTeX
@misc{bhattacharya2026unique,
  title = {{A unique intestinal epithelial cell that expresses canonical lymphoid genes}},
  author = {Swarnabh Bhattacharya and Krithika Badarinath and Guodong Tie and Pratik N. P. Singh and Onur Eskiocak and Ruiyang He and Bin Bao and Su-Hyung Lee and Scott Snapper and Kun Huang and James R. Goldenring and Semir Beyaz and Ramesh A. Shivdasani},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.21.760658},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.21.760658v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - A unique intestinal epithelial cell that expresses canonical lymphoid genes
AU  - Swarnabh Bhattacharya
AU  - Krithika Badarinath
AU  - Guodong Tie
AU  - Pratik N. P. Singh
AU  - Onur Eskiocak
AU  - Ruiyang He
AU  - Bin Bao
AU  - Su-Hyung Lee
AU  - Scott Snapper
AU  - Kun Huang
AU  - James R. Goldenring
AU  - Semir Beyaz
AU  - Ramesh A. Shivdasani
PY  - 2026
DA  - 2026/10/21
PB  - bioRxiv
DO  - 10.64898/2026.10.21.760658
UR  - https://www.biorxiv.org/content/10.64898/2026.10.21.760658v1
AB  - Enterocytes and intestinal secretory cells harbor innate immunity. However, certain transcriptional programs -even beyond rearranged T- and B-cell receptor genes- are considered the exclusive purview of adaptive immune cells, including abundant and diverse intestinal intra-epithelial lymphocytes (IELs). Here, we identify and characterize columnar intestinal secretory cells that co-express classic epithelial and lymphoid genes, including Ptprc, Cd3e, Cd8a, and Gzma/b but not T- or B-cell receptors. These previously unrecognized epithelium-derived lymphoid-like cells (EDLCs) differ from any known resident cell type, including IELs or tuft cells. Rare in healthy mouse and human intestines, their numbers increase upon IL-25 stimulation or IL-10 receptor deficiency in vivo and upon IL-13 stimulation in vitro. EDLC chromatin is uniquely accessible at thousands of cis-elements, including lymphoid and epithelial loci. These findings reveal a transcriptionally and epigenetically distinct intestinal epithelial derivative that expresses prominent lymphoid character.
SN  - 2692-8205
ER  -