Histopathologic spectrum of focal liver lesions diagnosed by ultrasound-guided percutaneous liver biopsy and predictors of hepatocellular carcinoma: A single-center experience from Sub-Saharan Africa
5 affiliations
- 1Department of Internal Medicine, University of Rwanda, Kigali, Rwanda
- 2Department of Internal Medicine, Adera Medical and Surgical Center, Addis Ababa, Ethiopia
- 3Department of Internal Medicine, Addis Ababa University, Addis Ababa, Ethiopia
- 4Department of Internal Medicine, Dilla University, Dilla, Ethiopia
- 5Department of Internal Medicine, St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia
Abstract
Background: Focal liver lesions (FLLs) encompass a wide spectrum of benign and malignant pathologies, and accurate diagnosis is essential for appropriate management. Although advances in imaging have improved lesion characterization, histopathologic assessment remains the diagnostic gold standard for indeterminate lesions. Data on the histopathologic spectrum and diagnostic utility of ultrasound-guided percutaneous liver biopsy (US-PLB) in sub-Saharan Africa (SSA) are limited. This study aimed to characterize the histopathologic findings of US-PLB performed for FLLs at a tertiary referral center in SSA and to identify factors associated with hepatocellular carcinoma (HCC). Methods: We conducted a retrospective observational study of adult patients (≥18 years) who underwent US-PLB for FLL between January 2021 and December 2024 at Adera Medical and Surgical Center. Patients with indeterminate pathology results, incomplete records, biopsies performed for diffuse liver disease, or lesions classified as LI-RADS 1, 2, or 5 were excluded. Demographic, clinical, laboratory, imaging, histopathologic, and outcome data were extracted from medical records. Descriptive statistics were used to summarize patient characteristics and histopathologic diagnoses. Logistic regression analysis was performed to identify factors associated with HCC. Results: A total of 119 were included in the final analysis. The median age was 56 years (IQR 45–65), and 59.7% were male. The median focal liver lesion size was 8.20 cm (IQR: 5.1–11.0 cm), with 38.7% presenting as multifocal lesions. No major biopsy-related complications were reported. HCC was the most common histopathologic diagnosis, accounting for 42.9% of cases, followed by secondary metastatic tumors (15.9%) and regenerative nodules (15.9%). Other diagnoses included chronic hepatitis (8.4%), cholangiocarcinoma (5.9%), and hepatic abscess (3.4%). Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections were present in 14.3% and 12.4% of patients, respectively. On multivariate analysis, HBV infection (AOR 7.85, 95% CI 1.45–42.60; p = 0.017), HCV infection (AOR 9.03, 95% CI 1.41–57.76; p = 0.020), Fibrosis-4 (FIB-4) score (AOR 1.45, 95% CI: 1.05–2.34: p < 0.01), and larger tumor size (AOR 1.27, 95% CI 1.11–1.46; p < 0.01) were significantly associated with HCC. Conclusion: Ultrasound-guided percutaneous liver biopsy demonstrated a favorable safety profile for the evaluation of FLL. HCC was the predominant histopathologic diagnosis, reflecting the substantial burden of primary liver cancer in this setting. Chronic viral hepatitis and larger tumor size were significantly associated with HCC. These findings support the continued role of US-PLB in the diagnostic evaluation of indeterminate focal liver lesions and underscores the importance of viral hepatitis prevention, surveillance, and early detection strategies in sub-Saharan Africa.
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BibTeX
@article{elias2026histopathologic,
title = {{Histopathologic spectrum of focal liver lesions diagnosed by ultrasound-guided percutaneous liver biopsy and predictors of hepatocellular carcinoma: A single-center experience from Sub-Saharan Africa}},
author = {Tamrat Petros Elias and Abate Bane Shewaye and Kaleb Assefa Berhane and Abdu Mohammed and Zebeaman Tibebu and Amanuel Getu Gebreselassie and Abraham Sisay Abie},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0357210},
url = {https://doi.org/10.1371/journal.pone.0357210},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Histopathologic spectrum of focal liver lesions diagnosed by ultrasound-guided percutaneous liver biopsy and predictors of hepatocellular carcinoma: A single-center experience from Sub-Saharan Africa
AU - Tamrat Petros Elias
AU - Abate Bane Shewaye
AU - Kaleb Assefa Berhane
AU - Abdu Mohammed
AU - Zebeaman Tibebu
AU - Amanuel Getu Gebreselassie
AU - Abraham Sisay Abie
PY - 2026
DA - 2026/10/16
JO - PLOS One
DO - 10.1371/journal.pone.0357210
UR - https://doi.org/10.1371/journal.pone.0357210
AB - Background: Focal liver lesions (FLLs) encompass a wide spectrum of benign and malignant pathologies, and accurate diagnosis is essential for appropriate management. Although advances in imaging have improved lesion characterization, histopathologic assessment remains the diagnostic gold standard for indeterminate lesions. Data on the histopathologic spectrum and diagnostic utility of ultrasound-guided percutaneous liver biopsy (US-PLB) in sub-Saharan Africa (SSA) are limited. This study aimed to characterize the histopathologic findings of US-PLB performed for FLLs at a tertiary referral center in SSA and to identify factors associated with hepatocellular carcinoma (HCC). Methods: We conducted a retrospective observational study of adult patients (≥18 years) who underwent US-PLB for FLL between January 2021 and December 2024 at Adera Medical and Surgical Center. Patients with indeterminate pathology results, incomplete records, biopsies performed for diffuse liver disease, or lesions classified as LI-RADS 1, 2, or 5 were excluded. Demographic, clinical, laboratory, imaging, histopathologic, and outcome data were extracted from medical records. Descriptive statistics were used to summarize patient characteristics and histopathologic diagnoses. Logistic regression analysis was performed to identify factors associated with HCC. Results: A total of 119 were included in the final analysis. The median age was 56 years (IQR 45–65), and 59.7% were male. The median focal liver lesion size was 8.20 cm (IQR: 5.1–11.0 cm), with 38.7% presenting as multifocal lesions. No major biopsy-related complications were reported. HCC was the most common histopathologic diagnosis, accounting for 42.9% of cases, followed by secondary metastatic tumors (15.9%) and regenerative nodules (15.9%). Other diagnoses included chronic hepatitis (8.4%), cholangiocarcinoma (5.9%), and hepatic abscess (3.4%). Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections were present in 14.3% and 12.4% of patients, respectively. On multivariate analysis, HBV infection (AOR 7.85, 95% CI 1.45–42.60; p = 0.017), HCV infection (AOR 9.03, 95% CI 1.41–57.76; p = 0.020), Fibrosis-4 (FIB-4) score (AOR 1.45, 95% CI: 1.05–2.34: p < 0.01), and larger tumor size (AOR 1.27, 95% CI 1.11–1.46; p < 0.01) were significantly associated with HCC. Conclusion: Ultrasound-guided percutaneous liver biopsy demonstrated a favorable safety profile for the evaluation of FLL. HCC was the predominant histopathologic diagnosis, reflecting the substantial burden of primary liver cancer in this setting. Chronic viral hepatitis and larger tumor size were significantly associated with HCC. These findings support the continued role of US-PLB in the diagnostic evaluation of indeterminate focal liver lesions and underscores the importance of viral hepatitis prevention, surveillance, and early detection strategies in sub-Saharan Africa.
SN - 1932-6203
ER -