Bioprocessing of Damaged Wheat Grain Into PHB Using Lactobacillus Fermentation Following Optimized Acid Hydrolysis
- 1Department of Food Science and Technology VaP.C., Islamic Azad University Varamin Iran
- 2Research Center of Food Technology and Agricultural Products Standard Research Institute (SRI) Karaj Iran
Abstract
This study establishes a sustainable strategy for poly(3‐hydroxybutyrate) (PHB) biosynthesis by utilizing damaged wheat grain (DWG) as an alternative, non‐food agro‐industrial substrate. The integrated approach involves the systematic optimization of HCl‐mediated hydrolysis followed by microbial fermentation using Lactobacillus species. Hydrolysis parameters, including time, acid‐to‐sample ratio, HCl concentration, and substrate loading, were evaluated to maximize reducing sugar (RS) release while suppressing the formation of fermentation inhibitors. Optimal nutrient recovery was achieved at 45 min, 20% w/v acid‐to‐sample ratio, 6% w/v HCl, and 20% w/w substrate loading, yielding 336.66 ± 1.14 mg glucose equivalent/g RS and 4.67% ± 0.07% w/w protein. Under these optimized conditions, inhibitory concentrations of acetic acid, formic acid, furfural, and 5‐HMF were effectively restricted to 2.36, 6.95, 0.49, and 1.17 mg/g, respectively. Subsequent fermentation of the refined hydrolysate by a consortium of Lactobacillus plantarum , Lactobacillus murinus , and Lactobacillus casei was conducted. At 30°C with a 6% inoculum level over 96 h, FH12 achieved a maximum PHB titer of 1.85 ± 0.42 g/L. These results demonstrate the potential of converting low‐value agro‐wastes into biopolymers via optimized lactic acid bacteria fermentation, providing a viable pathway for circular bioeconomy applications.
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BibTeX
@article{boorboormoradi2026bioprocessing,
title = {{Bioprocessing of Damaged Wheat Grain Into PHB Using Lactobacillus Fermentation Following Optimized Acid Hydrolysis}},
author = {Zohreh Boorboormoradi and Orang Eyvazzade and Ladan Rashidi and Leila Nateghi},
journal = {Food science \& nutrition},
year = {2026},
month = oct,
doi = {10.1002/fsn3.72491},
url = {https://europepmc.org/article/PMC/PMC13722947},
note = {CC BY},
}
RIS
TY - JOUR
TI - Bioprocessing of Damaged Wheat Grain Into PHB Using Lactobacillus Fermentation Following Optimized Acid Hydrolysis
AU - Zohreh Boorboormoradi
AU - Orang Eyvazzade
AU - Ladan Rashidi
AU - Leila Nateghi
PY - 2026
DA - 2026/10/31
JO - Food science & nutrition
DO - 10.1002/fsn3.72491
UR - https://europepmc.org/article/PMC/PMC13722947
AB - This study establishes a sustainable strategy for poly(3‐hydroxybutyrate) (PHB) biosynthesis by utilizing damaged wheat grain (DWG) as an alternative, non‐food agro‐industrial substrate. The integrated approach involves the systematic optimization of HCl‐mediated hydrolysis followed by microbial fermentation using Lactobacillus species. Hydrolysis parameters, including time, acid‐to‐sample ratio, HCl concentration, and substrate loading, were evaluated to maximize reducing sugar (RS) release while suppressing the formation of fermentation inhibitors. Optimal nutrient recovery was achieved at 45 min, 20% w/v acid‐to‐sample ratio, 6% w/v HCl, and 20% w/w substrate loading, yielding 336.66 ± 1.14 mg glucose equivalent/g RS and 4.67% ± 0.07% w/w protein. Under these optimized conditions, inhibitory concentrations of acetic acid, formic acid, furfural, and 5‐HMF were effectively restricted to 2.36, 6.95, 0.49, and 1.17 mg/g, respectively. Subsequent fermentation of the refined hydrolysate by a consortium of Lactobacillus plantarum , Lactobacillus murinus , and Lactobacillus casei was conducted. At 30°C with a 6% inoculum level over 96 h, FH12 achieved a maximum PHB titer of 1.85 ± 0.42 g/L. These results demonstrate the potential of converting low‐value agro‐wastes into biopolymers via optimized lactic acid bacteria fermentation, providing a viable pathway for circular bioeconomy applications.
SN - 2048-7177
ER -