Complex-trait dissection with Evolve-and-Resequence: Selection for improved flight performance in laboratory fruit fly populations
- 1University of Missouri
Abstract
Evolve-and-Resequence studies combine experimental evolution with molecular sequencing, enabling associations between complex trait phenotypes and evolved genomic changes. For this, a targeted complex trait is subjected to artificial laboratory selection in genetically variable populations. Using populations of fruit flies Drosophila melanogaster from the DSPR background, we have conducted a short-term selection study on a highly complex trait : flight performance. We selected for improved flight performance using a custom-built flight tunnel system, with light as a motivator and airflow as a resistive factor. As a result, flight performance significantly improved in selected populations compared to controls. We also observed signatures of evolution in flight related traits, such as activity level and wing shape, and in life-history related traits such as adult dry mass and fecundity. We used whole genome pooled sequencing on the selected and control populations, revealing significantly differentiated genomic sites. Gene ontology and enrichment analyses revealed the evolution of pathways involved in response to stimuli, growth, and development. We identified several genes of interest (e.g., Sdc, dally). The range of evolved phenotypes and associated genomic changes show that flight performance is an ideal complex trait for future Evolve-and-Resequence studies.
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BibTeX
@misc{venkitachalam2026complextrait,
title = {{Complex-trait dissection with Evolve-and-Resequence: Selection for improved flight performance in laboratory fruit fly populations}},
author = {S. Venkitachalam and N. A. Heath and A. Albright and M. York and M. Kuehnel and K. Robinson and S. Vazquez and K. M. Middleton and E. G. King},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.15.759414},
url = {https://www.biorxiv.org/content/10.64898/2026.10.15.759414v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Complex-trait dissection with Evolve-and-Resequence: Selection for improved flight performance in laboratory fruit fly populations
AU - S. Venkitachalam
AU - N. A. Heath
AU - A. Albright
AU - M. York
AU - M. Kuehnel
AU - K. Robinson
AU - S. Vazquez
AU - K. M. Middleton
AU - E. G. King
PY - 2026
DA - 2026/10/15
PB - bioRxiv
DO - 10.64898/2026.10.15.759414
UR - https://www.biorxiv.org/content/10.64898/2026.10.15.759414v1
AB - Evolve-and-Resequence studies combine experimental evolution with molecular sequencing, enabling associations between complex trait phenotypes and evolved genomic changes. For this, a targeted complex trait is subjected to artificial laboratory selection in genetically variable populations. Using populations of fruit flies Drosophila melanogaster from the DSPR background, we have conducted a short-term selection study on a highly complex trait : flight performance. We selected for improved flight performance using a custom-built flight tunnel system, with light as a motivator and airflow as a resistive factor. As a result, flight performance significantly improved in selected populations compared to controls. We also observed signatures of evolution in flight related traits, such as activity level and wing shape, and in life-history related traits such as adult dry mass and fecundity. We used whole genome pooled sequencing on the selected and control populations, revealing significantly differentiated genomic sites. Gene ontology and enrichment analyses revealed the evolution of pathways involved in response to stimuli, growth, and development. We identified several genes of interest (e.g., Sdc, dally). The range of evolved phenotypes and associated genomic changes show that flight performance is an ideal complex trait for future Evolve-and-Resequence studies.
ER -