Molecular and morphological characterization of nine new Gnomoniopsis species (Diaporthales, Gnomoniaceae): Genomic insights into evolution and phylogeny
- 1College of Life Sciences, Shandong Normal University, Jinan, 250358, China College of Life Sciences, Shandong Normal University Jinan China https://ror.org/01wy3h363
- 2Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University Taian China https://ror.org/02ke8fw32
Abstract
Gnomoniopsis comprises parasitic, endophytic, and saprophytic fungi. Since its establishment, this genus has presented considerable taxonomic challenges owing to extensive morphological overlap among species, rendering traditional classification systems that rely predominantly on phenotypic characteristics no longer applicable. To resolve the current taxonomic confusion and clarify the species diversity, phylogenetic relationships, and genomic adaptive characteristics of Gnomoniopsis, the present study was carried out. In this study, the internal transcriptional spacer (ITS), translation extension factor 1-α (tef1), and partial beta-tubulin (tub2) were analyzed and combined to observe morphological characteristics of Gnomoniopsis. The isolates were identified from chestnut (Castanea), Embelia vestita, tea (Camellia sinensis), and other hosts in Sichuan Province, as well as from Quercus species and partial leaf rot from Fujian Province. Nine new species, namely G. xiaoheijiangensissp. nov., G. embeliaesp. nov., G. yunvfengensissp. nov., G. quercussp. nov., G. ledongensissp. nov., G. yonganensissp. nov., G. conghuaensissp. nov., G. loropetalisp. nov., and G. qixiensissp. nov., were obtained. Phylogenomic analysis based on 7,942 orthologous proteins’ clusters revealed distinct species boundaries within Gnomoniopsis. Phylogenomics has thus effectively resolved the ambiguous phylogenetic relationships in this genus. This work identifies nine previously undescribed Gnomoniopsis species, clarifies the species diversity and conserved genomic characteristics within the genus, and lays a foundation for subsequent taxonomic analyses of Gnomoniopsis.
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BibTeX
@article{wang2026molecular,
title = {{Molecular and morphological characterization of nine new Gnomoniopsis species (Diaporthales, Gnomoniaceae): Genomic insights into evolution and phylogeny}},
author = {Xingsheng Wang and Yang Jiang and Wenwen Liu and Xigang Yan and Jinyan Sheng and Congcong Ai and Xiaoyong Liu and Xiuguo Zhang and Zhe Meng and Shi Wang},
journal = {IMA Fungus},
year = {2026},
month = oct,
doi = {10.3897/imafungus.17.208364},
url = {https://doi.org/10.3897/imafungus.17.208364},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Molecular and morphological characterization of nine new Gnomoniopsis species (Diaporthales, Gnomoniaceae): Genomic insights into evolution and phylogeny
AU - Xingsheng Wang
AU - Yang Jiang
AU - Wenwen Liu
AU - Xigang Yan
AU - Jinyan Sheng
AU - Congcong Ai
AU - Xiaoyong Liu
AU - Xiuguo Zhang
AU - Zhe Meng
AU - Shi Wang
PY - 2026
DA - 2026/10/23
JO - IMA Fungus
DO - 10.3897/imafungus.17.208364
UR - https://doi.org/10.3897/imafungus.17.208364
AB - Gnomoniopsis comprises parasitic, endophytic, and saprophytic fungi. Since its establishment, this genus has presented considerable taxonomic challenges owing to extensive morphological overlap among species, rendering traditional classification systems that rely predominantly on phenotypic characteristics no longer applicable. To resolve the current taxonomic confusion and clarify the species diversity, phylogenetic relationships, and genomic adaptive characteristics of Gnomoniopsis, the present study was carried out. In this study, the internal transcriptional spacer (ITS), translation extension factor 1-α (tef1), and partial beta-tubulin (tub2) were analyzed and combined to observe morphological characteristics of Gnomoniopsis. The isolates were identified from chestnut (Castanea), Embelia vestita, tea (Camellia sinensis), and other hosts in Sichuan Province, as well as from Quercus species and partial leaf rot from Fujian Province. Nine new species, namely G. xiaoheijiangensissp. nov., G. embeliaesp. nov., G. yunvfengensissp. nov., G. quercussp. nov., G. ledongensissp. nov., G. yonganensissp. nov., G. conghuaensissp. nov., G. loropetalisp. nov., and G. qixiensissp. nov., were obtained. Phylogenomic analysis based on 7,942 orthologous proteins’ clusters revealed distinct species boundaries within Gnomoniopsis. Phylogenomics has thus effectively resolved the ambiguous phylogenetic relationships in this genus. This work identifies nine previously undescribed Gnomoniopsis species, clarifies the species diversity and conserved genomic characteristics within the genus, and lays a foundation for subsequent taxonomic analyses of Gnomoniopsis.
SN - 2210-6340
SN - 2210-6359
ER -