Multiregional single cell profiling reveals LGALS9 and CD44 mediated niche remodeling crosstalk in metastatic breast cancer
Abstract
The transition from intraductal to invasive Breast cancer (BC) is driven by complex interactions within the tumor microenvironment (TME). Cancer-associated fibroblasts (CAFs), a key stromal component, critically influence extracellular matrix remodeling, immune modulation, and metastatic dissemination. However, the spatially resolved cellular mechanisms through which malignant epithelial subpopulations reprogram fibroblasts to promote metastasis remain incompletely understood. We performed multiregional single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics on breast cancer specimens representing the tumor core, tumor border, and adjacent normal tissue. Malignant mammary luminal (ML) subtypes were identified through Large-scale chromosomal copy number variation (CNV) profiling. Fibroblast sub-clustering, pseudotime trajectory reconstruction, and cell–cell communication analysis (Cell Chat) were integrated with in vitro functional assays using primary fibroblasts and breast cancer cell lines to delineate key signaling interactions. Five ML clusters were identified, among which ML4—enriched at tumor borders—displayed transcriptional programs linked to ribosome activation, hypoxia, and hyper-inflammatory, and was associated with high recurrence and metastasis in TCGA-BC. Fibroblasts were classified into three spatially distinct subtypes: myofibroblasts (myoFIB), inflammatory fibroblasts (iFIB), and APOD⁺PTGDS⁺ fibroblasts, with iFIB predominantly localized to tumor borders. Cell–cell interaction analysis revealed hyperactivation of the LGALS9 (tumor)–CD44 (fibroblast) signaling associated pathway in the border regions. Functional validation confirmed that LGALS9-expressing BC cells promote migration and activation of primary fibroblasts, whereas activated fibroblasts enhance epithelial–mesenchymal transition and migration of BC cells, potentially involving via Smad2/3 signaling. We identify a spatially restricted malignant luminal subtype, ML4, which is associated with fibroblast activation through LGALS9–CD44 signaling, suggesting bidirectional crosstalk between breast cancer cells and fibroblasts within the TME. This niche remodeling holds prognostic relevance and may contribute to metastatic progression, representing a potentially targetable pathway requiring further validation in aggressive breast cancer.
Versions
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BibTeX
@article{tang2026multiregional,
title = {{Multiregional single cell profiling reveals LGALS9 and CD44 mediated niche remodeling crosstalk in metastatic breast cancer}},
author = {Xun Tang and Ning Jiang and Yingxue Cui and Jianjiang Shen and Hui Lu and Feng Yan},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-72151-4},
url = {https://www.nature.com/articles/s41598-026-72151-4},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Multiregional single cell profiling reveals LGALS9 and CD44 mediated niche remodeling crosstalk in metastatic breast cancer
AU - Xun Tang
AU - Ning Jiang
AU - Yingxue Cui
AU - Jianjiang Shen
AU - Hui Lu
AU - Feng Yan
PY - 2026
DA - 2026/10/15
JO - Scientific Reports
DO - 10.1038/s41598-026-72151-4
UR - https://www.nature.com/articles/s41598-026-72151-4
AB - The transition from intraductal to invasive Breast cancer (BC) is driven by complex interactions within the tumor microenvironment (TME). Cancer-associated fibroblasts (CAFs), a key stromal component, critically influence extracellular matrix remodeling, immune modulation, and metastatic dissemination. However, the spatially resolved cellular mechanisms through which malignant epithelial subpopulations reprogram fibroblasts to promote metastasis remain incompletely understood. We performed multiregional single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics on breast cancer specimens representing the tumor core, tumor border, and adjacent normal tissue. Malignant mammary luminal (ML) subtypes were identified through Large-scale chromosomal copy number variation (CNV) profiling. Fibroblast sub-clustering, pseudotime trajectory reconstruction, and cell–cell communication analysis (Cell Chat) were integrated with in vitro functional assays using primary fibroblasts and breast cancer cell lines to delineate key signaling interactions. Five ML clusters were identified, among which ML4—enriched at tumor borders—displayed transcriptional programs linked to ribosome activation, hypoxia, and hyper-inflammatory, and was associated with high recurrence and metastasis in TCGA-BC. Fibroblasts were classified into three spatially distinct subtypes: myofibroblasts (myoFIB), inflammatory fibroblasts (iFIB), and APOD⁺PTGDS⁺ fibroblasts, with iFIB predominantly localized to tumor borders. Cell–cell interaction analysis revealed hyperactivation of the LGALS9 (tumor)–CD44 (fibroblast) signaling associated pathway in the border regions. Functional validation confirmed that LGALS9-expressing BC cells promote migration and activation of primary fibroblasts, whereas activated fibroblasts enhance epithelial–mesenchymal transition and migration of BC cells, potentially involving via Smad2/3 signaling. We identify a spatially restricted malignant luminal subtype, ML4, which is associated with fibroblast activation through LGALS9–CD44 signaling, suggesting bidirectional crosstalk between breast cancer cells and fibroblasts within the TME. This niche remodeling holds prognostic relevance and may contribute to metastatic progression, representing a potentially targetable pathway requiring further validation in aggressive breast cancer.
SN - 2045-2322
ER -