Rapid identification of acrolein scavengers from Anoectochilus roxburghii and evaluation of their representative antiglycation and antioxidant activities.
- 1Dr. Neher's Biophysics Laboratory for Innovative Drug Discovery, The State Key Laboratory of Mechanism and Quality of Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, China.
- 2Macau University of Science and Technology Zhuhai MUST Science and Technology Research Institute, China.
Abstract
Acrolein (ACR), a highly reactive α,β-unsaturated carbonyl compound generated during thermal food processing and lipid peroxidation, contributes to carbonyl stress and oxidative protein modification. In this study, a reaction-guided UHPLC-DAD-HRMS/MS strategy was developed to screen natural ACR scavengers from Anoectochilus roxburghii (A. roxburghii). Eight major ACR-reactive compounds were identified, and narcissoside (NAR) was selected for further validation due to its relatively high abundance and confirmed ACR reactivity. NAR directly trapped ACR in vitro through mono-, di-, and tri-adduct formation, while in vivo fecal analysis suggested that its metabolite isorhamnetin (ISO) contributed to ACR trapping. NAR also attenuated protein modification in ACR-BSA, MGO-BSA, Glu-BSA, and Fru-BSA models. Furthermore, low-dose subacute ACR exposure showed that NAR and ISO improved serum carbonyl stress, oxidative stress, and inflammatory markers. These findings identify NAR as a representative ACR-trapping constituent of A. roxburghii.
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BibTeX
@article{zhuang2026rapid,
title = {{Rapid identification of acrolein scavengers from Anoectochilus roxburghii and evaluation of their representative antiglycation and antioxidant activities.}},
author = {Guo Dong Zhuang and Yong Pei Tang and Rui Long Zhang and Hang Hong Lo and Lin Lin Song and Xiao Yun Yun and Jian Hui Wu and Cai Ren Wang and Hui Miao Wang and Meng Han Liu and Zhi Kang Wang and Vincent Kam Wai Wong and Betty Yuen Kwan Law},
journal = {Food chemistry: X},
year = {2026},
month = oct,
doi = {10.1016/j.fochx.2026.104585},
url = {https://europepmc.org/article/MED/42898581},
note = {CC BY},
}
RIS
TY - JOUR
TI - Rapid identification of acrolein scavengers from Anoectochilus roxburghii and evaluation of their representative antiglycation and antioxidant activities.
AU - Guo Dong Zhuang
AU - Yong Pei Tang
AU - Rui Long Zhang
AU - Hang Hong Lo
AU - Lin Lin Song
AU - Xiao Yun Yun
AU - Jian Hui Wu
AU - Cai Ren Wang
AU - Hui Miao Wang
AU - Meng Han Liu
AU - Zhi Kang Wang
AU - Vincent Kam Wai Wong
AU - Betty Yuen Kwan Law
PY - 2026
DA - 2026/10/07
JO - Food chemistry: X
DO - 10.1016/j.fochx.2026.104585
UR - https://europepmc.org/article/MED/42898581
AB - Acrolein (ACR), a highly reactive α,β-unsaturated carbonyl compound generated during thermal food processing and lipid peroxidation, contributes to carbonyl stress and oxidative protein modification. In this study, a reaction-guided UHPLC-DAD-HRMS/MS strategy was developed to screen natural ACR scavengers from Anoectochilus roxburghii (A. roxburghii). Eight major ACR-reactive compounds were identified, and narcissoside (NAR) was selected for further validation due to its relatively high abundance and confirmed ACR reactivity. NAR directly trapped ACR in vitro through mono-, di-, and tri-adduct formation, while in vivo fecal analysis suggested that its metabolite isorhamnetin (ISO) contributed to ACR trapping. NAR also attenuated protein modification in ACR-BSA, MGO-BSA, Glu-BSA, and Fru-BSA models. Furthermore, low-dose subacute ACR exposure showed that NAR and ISO improved serum carbonyl stress, oxidative stress, and inflammatory markers. These findings identify NAR as a representative ACR-trapping constituent of A. roxburghii.
SN - 2590-1575
ER -