Phenotypic and genomic characterization of a halophilic sulfate-reducing bacterium Desulfoplanes aerotolerans sp. nov. from a petroleum reservoir and its possible implication in pipeline corrosion
4 affiliations
- 1Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
- 2School of Marine and Atmospheric Sciences, Stony Brook University
- 3Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- 4Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences
Abstract
Introduction: The issue of biogenic corrosion of steel equipment is especially pressing for petroleum reservoirs with high-salinity formation water containing sulfate. The predominance of sulfate-reducing bacteria of the genus Desulfoplanes in formation water samples from petroleum reservoirs in Tatarstan (Russia) was previously demonstrated using 16S rRNA gene amplicon sequencing and metagenomic analysis. The present study is devoted to the isolation, phenotypic and genomic characterization of the sulfate-reducing bacterial strain Desulfoplanes sp. PS50 T and its involvement in steel corrosion. Studying the composition of sulfidogenic prokaryotes and their corrosion activity is necessary in order to find a way to suppress their growth and minimize corrosion of oilfield equipment.
Methods: A new sulfate-reducing bacterial strain Desulfoplanes sp. PS50 T was studied using polyphase taxonomy approaches, including genome sequencing and functional annotation. The ability to cause corrosion of the steel coupon and the effect of three biocides on the growth of the strain were tested.
Results: Sulfate-reducing strain Desulfoplanes sp. PS50 T isolated from an Arkhangelskoe petroleum reservoir (Tatarstan, Russia) was phenotypically characterized. The optimal growth of the strain PS50 T was observed at a temperature of 25–30 °C, in the presence of 40–50 g NaCl·L ⁻¹ and at pH 7.5. The strain utilized formate, lactate, yeast extract, and H ₂ /CO ₂ /acetate as carbon and energy sources for reduction of sulfate to sulfide. The strain exhibited both anaerobic and microaerophilic growth (in the presence of 5% O ₂ ( v/v ). Based on physiological features and genomic and phylogenomic analyses, strain PS50 T (=VKM B-4014 T = KCTC 35101 T ) has been assigned to a new species, Desulfoplanes aerotolerans sp. nov. Genomic analysis revealed genes of sulfate reduction, hydrogenase, and iron uptake, which confirms the strain’s ability to participate in the steel biocorrosion. The genome also contains the genes that determine the main metabolic pathways and the strain’s adaptation to the high-salinity conditions of its habitat.
Discussion: This study provides experimental proofs on the ability of sulfate-reducing bacteria of the genus Desulfoplanes to participate in general and pitting corrosion, confirmed by deep genome-resolved detection of corrosion-determining genes.
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BibTeX
@article{bidzhieva2026phenotypic,
title = {{Phenotypic and genomic characterization of a halophilic sulfate-reducing bacterium Desulfoplanes aerotolerans sp. nov. from a petroleum reservoir and its possible implication in pipeline corrosion}},
author = {Salimat K. Bidzhieva and Tatiana P. Tourova and Denis S. Grouzdev and Salima R. Soloveva and Diyana S. Sokolova and Vera M. Tereshina and Andrey B. Poltaraus and Andrey V. Mardanov and Tamara N. Nazina},
journal = {Frontiers in Microbiology},
year = {2026},
month = oct,
doi = {10.3389/fmicb.2026.1968038},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1968038/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Phenotypic and genomic characterization of a halophilic sulfate-reducing bacterium Desulfoplanes aerotolerans sp. nov. from a petroleum reservoir and its possible implication in pipeline corrosion
AU - Salimat K. Bidzhieva
AU - Tatiana P. Tourova
AU - Denis S. Grouzdev
AU - Salima R. Soloveva
AU - Diyana S. Sokolova
AU - Vera M. Tereshina
AU - Andrey B. Poltaraus
AU - Andrey V. Mardanov
AU - Tamara N. Nazina
PY - 2026
DA - 2026/10/14
JO - Frontiers in Microbiology
DO - 10.3389/fmicb.2026.1968038
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1968038/full
AB - Introduction: The issue of biogenic corrosion of steel equipment is especially pressing for petroleum reservoirs with high-salinity formation water containing sulfate. The predominance of sulfate-reducing bacteria of the genus Desulfoplanes in formation water samples from petroleum reservoirs in Tatarstan (Russia) was previously demonstrated using 16S rRNA gene amplicon sequencing and metagenomic analysis. The present study is devoted to the isolation, phenotypic and genomic characterization of the sulfate-reducing bacterial strain Desulfoplanes sp. PS50 T and its involvement in steel corrosion. Studying the composition of sulfidogenic prokaryotes and their corrosion activity is necessary in order to find a way to suppress their growth and minimize corrosion of oilfield equipment. Methods: A new sulfate-reducing bacterial strain Desulfoplanes sp. PS50 T was studied using polyphase taxonomy approaches, including genome sequencing and functional annotation. The ability to cause corrosion of the steel coupon and the effect of three biocides on the growth of the strain were tested. Results: Sulfate-reducing strain Desulfoplanes sp. PS50 T isolated from an Arkhangelskoe petroleum reservoir (Tatarstan, Russia) was phenotypically characterized. The optimal growth of the strain PS50 T was observed at a temperature of 25–30 °C, in the presence of 40–50 g NaCl·L ⁻¹ and at pH 7.5. The strain utilized formate, lactate, yeast extract, and H ₂ /CO ₂ /acetate as carbon and energy sources for reduction of sulfate to sulfide. The strain exhibited both anaerobic and microaerophilic growth (in the presence of 5% O ₂ ( v/v ). Based on physiological features and genomic and phylogenomic analyses, strain PS50 T (=VKM B-4014 T = KCTC 35101 T ) has been assigned to a new species, Desulfoplanes aerotolerans sp. nov. Genomic analysis revealed genes of sulfate reduction, hydrogenase, and iron uptake, which confirms the strain’s ability to participate in the steel biocorrosion. The genome also contains the genes that determine the main metabolic pathways and the strain’s adaptation to the high-salinity conditions of its habitat. Discussion: This study provides experimental proofs on the ability of sulfate-reducing bacteria of the genus Desulfoplanes to participate in general and pitting corrosion, confirmed by deep genome-resolved detection of corrosion-determining genes.
SN - 1664-302X
ER -