Plasticity-induced actin polymerization positions endocytic compartments at the bases of stimulated spines
- 1Janelia Research Campus
Abstract
The endocytic pathway plays key roles in facilitating and regulating synaptic plasticity, but how its components are organized at spines undergoing plasticity is not fully understood. Here we use live-cell imaging and single-particle tracking to examine the localization of endosomes and lysosomes in response to plasticity-inducing stimuli. We find that endosomes and lysosomes are positioned and retained near the bases of stimulated spines after the induction of structural plasticity in a manner dependent on actin polymerization in the dendritic shaft. Spines with endocytic compartments near their bases exhibit greater glutamate receptor turnover and more sustained enlargement after stimulation than spines lacking them. These results suggest that actin polymerization in the dendritic shaft acts as a hub to organize critical cellular machinery at the bases of spines undergoing synaptic plasticity.
Related
- Role of Very Long-Chain Fatty Acyl-Ceramides in Endocytic Uptake
- P4-ATPase BbCrpa2 detoxifies cyclosporine a via AP2-clathrin-dependent endocytic pathway in Beauveria bassiana
- Dysregulation of miR-103a-3p/AGFG1 axis links endocytic trafficking dysfunction to ER stress in Parkinson's disease
- Dystrophic changes of nigrostriatal axons harboring a Synj1 Parkinson mutation suggest catastrophic failure of endocytic mechanisms
- Synaptotagmin-mediated plasma membrane homeostasis is required to recruit the endocytic machinery during cold stress
BibTeX
@misc{wong2026plasticityinduced,
title = {{Plasticity-induced actin polymerization positions endocytic compartments at the bases of stimulated spines}},
author = {V. C. Wong and D. Walpita and Z. J. Liu and E. K. O'Shea},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.08.756800},
url = {https://www.biorxiv.org/content/10.64898/2026.10.08.756800v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Plasticity-induced actin polymerization positions endocytic compartments at the bases of stimulated spines
AU - V. C. Wong
AU - D. Walpita
AU - Z. J. Liu
AU - E. K. O'Shea
PY - 2026
DA - 2026/10/08
PB - bioRxiv
DO - 10.64898/2026.10.08.756800
UR - https://www.biorxiv.org/content/10.64898/2026.10.08.756800v1
AB - The endocytic pathway plays key roles in facilitating and regulating synaptic plasticity, but how its components are organized at spines undergoing plasticity is not fully understood. Here we use live-cell imaging and single-particle tracking to examine the localization of endosomes and lysosomes in response to plasticity-inducing stimuli. We find that endosomes and lysosomes are positioned and retained near the bases of stimulated spines after the induction of structural plasticity in a manner dependent on actin polymerization in the dendritic shaft. Spines with endocytic compartments near their bases exhibit greater glutamate receptor turnover and more sustained enlargement after stimulation than spines lacking them. These results suggest that actin polymerization in the dendritic shaft acts as a hub to organize critical cellular machinery at the bases of spines undergoing synaptic plasticity.
ER -