Efficient preparation of fatty acid methyl esters using liquid lipase from thermotolerant fungus Rasamsonia emersonii via synergistic strategies
- 1College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023 Hubei China
Abstract
Lipase-catalyzed biodiesel preparation in non-aqueous media has the advantages of reduced energy consumption, simplified recovery of fatty acid methyl esters (FAMEs), and minimized environment pollution, etc. To discover a novel thermophilic lipase, achieve its high-level expression, and develop it as an efficient liquid biocatalyst for FAMEs production via methanolysis of plant oils in non-aqueous media, a lipase gene from the thermophilic fungus Rasamsonia emersonii was cloned and efficiently expressed through synergistic strategies including computational codon optimization and increased gene dosage in the host genome. The resulting liquid lipase preparation was directly employed as a biocatalyst for FAMEs production. Under optimized reaction conditions, a conversion rate of approximately 95% was achieved. This study provides a feasible approach for the large-scale production of thermophilic lipase. The strategy of directly using of liquid lipase as a biocatalyst for FAMEs preparation substantially simplifies enzyme immobilization, regeneration, and product recovery processes, offering an efficient alternative for enzymatic FAMEs production.
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BibTeX
@article{yang2026efficient,
title = {{Efficient preparation of fatty acid methyl esters using liquid lipase from thermotolerant fungus Rasamsonia emersonii via synergistic strategies}},
author = {Jiang-Ke Yang and Ying Han and Lin-Jun Wei and De-Ming Rao and Lei Lei},
journal = {Biotechnology for Biofuels and Bioproducts},
year = {2026},
month = aug,
doi = {10.1186/s13068-026-02801-2},
url = {https://doi.org/10.1186/s13068-026-02801-2},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Efficient preparation of fatty acid methyl esters using liquid lipase from thermotolerant fungus Rasamsonia emersonii via synergistic strategies
AU - Jiang-Ke Yang
AU - Ying Han
AU - Lin-Jun Wei
AU - De-Ming Rao
AU - Lei Lei
PY - 2026
DA - 2026/08/01
JO - Biotechnology for Biofuels and Bioproducts
DO - 10.1186/s13068-026-02801-2
UR - https://doi.org/10.1186/s13068-026-02801-2
AB - Lipase-catalyzed biodiesel preparation in non-aqueous media has the advantages of reduced energy consumption, simplified recovery of fatty acid methyl esters (FAMEs), and minimized environment pollution, etc. To discover a novel thermophilic lipase, achieve its high-level expression, and develop it as an efficient liquid biocatalyst for FAMEs production via methanolysis of plant oils in non-aqueous media, a lipase gene from the thermophilic fungus Rasamsonia emersonii was cloned and efficiently expressed through synergistic strategies including computational codon optimization and increased gene dosage in the host genome. The resulting liquid lipase preparation was directly employed as a biocatalyst for FAMEs production. Under optimized reaction conditions, a conversion rate of approximately 95% was achieved. This study provides a feasible approach for the large-scale production of thermophilic lipase. The strategy of directly using of liquid lipase as a biocatalyst for FAMEs preparation substantially simplifies enzyme immobilization, regeneration, and product recovery processes, offering an efficient alternative for enzymatic FAMEs production.
SN - 2731-3654
ER -