Probiotic strain released-carbamoyl phosphates modify corticosterone metabolism in colonic epithelial cells by elevating 11-beta-hydroxysteroid dehydrogenase isozyme 2
Abstract
Chronic psychological stress contributes to functional disorder development, including irritable bowel syndrome (IBS). Although probiotics have shown potential in ameliorating these disorders, the precise mechanisms remain incompletely understood. The aim of this study was to elucidate the mechanism underlying the effect of Lacticaseibacillus paracasei strain Shirota (LcS) on the host anti-stress response in a colonic epithelial cell line. Expression of the stress hormone-degrading enzyme, 11 β-hydroxysteroid dehydrogenase type 2 (Hsd11b2), was suppressed by corticosterone and restored by LcS treatment. Fractionation of LcS culture supernatant revealed a derivative of carbamoyl phosphate as the key factor responsible for inducing Hsd11b2. Moreover, activation of acetylcholine receptor and inhibition of NF-κB p50 homodimer nuclear translocation were required to induce Hsd11b2 in colonic epithelial cells. These findings reveal a novel probiotic mechanism whereby an LcS metabolite triggers anti-stress responses, including Hsd11b2 induction, by modulating the acetylcholine and NF-κB pathways. This new mechanism by which probiotics can stimulate anti-stress effects in the colonic mucosa may contribute to IBS treatment.
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BibTeX
@article{arase2026probiotic,
title = {{Probiotic strain released-carbamoyl phosphates modify corticosterone metabolism in colonic epithelial cells by elevating 11-beta-hydroxysteroid dehydrogenase isozyme 2}},
author = {Sohei ARASE and Minoru ANDO and Kosuke OANA and Yuji FUKUHARA and Takashi KURAKAWA and Tetsuji HORI and Satoshi MATSUMOTO},
journal = {Bioscience of Microbiota, Food and Health},
year = {2026},
month = sep,
doi = {10.12938/bmfh.2026-008},
url = {https://www.jstage.jst.go.jp/article/bmfh/advpub/0/advpub_2026-008/_article},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Probiotic strain released-carbamoyl phosphates modify corticosterone metabolism in colonic epithelial cells by elevating 11-beta-hydroxysteroid dehydrogenase isozyme 2
AU - Sohei ARASE
AU - Minoru ANDO
AU - Kosuke OANA
AU - Yuji FUKUHARA
AU - Takashi KURAKAWA
AU - Tetsuji HORI
AU - Satoshi MATSUMOTO
PY - 2026
DA - 2026/09/14
JO - Bioscience of Microbiota, Food and Health
DO - 10.12938/bmfh.2026-008
UR - https://www.jstage.jst.go.jp/article/bmfh/advpub/0/advpub_2026-008/_article
AB - Chronic psychological stress contributes to functional disorder development, including irritable bowel syndrome (IBS). Although probiotics have shown potential in ameliorating these disorders, the precise mechanisms remain incompletely understood. The aim of this study was to elucidate the mechanism underlying the effect of Lacticaseibacillus paracasei strain Shirota (LcS) on the host anti-stress response in a colonic epithelial cell line. Expression of the stress hormone-degrading enzyme, 11 β-hydroxysteroid dehydrogenase type 2 (Hsd11b2), was suppressed by corticosterone and restored by LcS treatment. Fractionation of LcS culture supernatant revealed a derivative of carbamoyl phosphate as the key factor responsible for inducing Hsd11b2. Moreover, activation of acetylcholine receptor and inhibition of NF-κB p50 homodimer nuclear translocation were required to induce Hsd11b2 in colonic epithelial cells. These findings reveal a novel probiotic mechanism whereby an LcS metabolite triggers anti-stress responses, including Hsd11b2 induction, by modulating the acetylcholine and NF-κB pathways. This new mechanism by which probiotics can stimulate anti-stress effects in the colonic mucosa may contribute to IBS treatment.
SN - 2186-3342
ER -