Insecticidal and antifeedant activity of cadalene isolated from Cyperus rotundus against fall armyworm (Spodoptera frugiperda)
Abstract
The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a highly destructive and polyphagous pest that has developed resistance to several classes of synthetic insecticides. This study evaluated the insecticidal and antifeedant activities of Cyperus rotundus extracts against S. frugiperda larvae and aimed to identify bioactive compounds with potential for development as botanical insecticides. Among the tested solvent fractions, the dichloromethane extract (LD₅₀ = 4.58 μg/larva) and the isolated compound cadalene (LD₅₀ = 4.12 μg/larva) exhibited insecticidal activity against S. frugiperda larvae. The positive control cypermethrin showed an LD₅₀ value of 0.01 μg/larva, indicating that cadalene exhibited notable toxicity relative to the conventional insecticide. Enzyme assays revealed significant inhibition of carboxylesterase and glutathione Stransferase activities, suggesting interference with detoxification pathways. Chromatographic analysis identified cadalene as the bioactive compound. In feeding assays, the hexane extract exhibited the strongest antifeedant activity, with an FI₅₀ value of 17.13 µg/cm² at 2 h post-treatment. Larvae feeding on treated diets showed delayed development and increased mortality. These findings highlight the potential of compounds derived from C. rotundus as promising plant-based candidates for the development of botanical insecticides for sustainable pest management.
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BibTeX
@article{tipsut2026insecticidal,
title = {{Insecticidal and antifeedant activity of cadalene isolated from Cyperus rotundus against fall armyworm (Spodoptera frugiperda)}},
author = {Nattarat Tipsut and Karit Pudchimnun and Lalita Pimsawang and Suchanan Phraoprakhon and Wanchai Pluempanupat and Gaëlle Le Goff and Vasakorn Bullangpoti},
journal = {Journal of Natural Pesticide Research},
year = {2026},
month = may,
doi = {10.1016/j.napere.2026.100201},
url = {https://hal.science/hal-05783420v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Insecticidal and antifeedant activity of cadalene isolated from Cyperus rotundus against fall armyworm (Spodoptera frugiperda)
AU - Nattarat Tipsut
AU - Karit Pudchimnun
AU - Lalita Pimsawang
AU - Suchanan Phraoprakhon
AU - Wanchai Pluempanupat
AU - Gaëlle Le Goff
AU - Vasakorn Bullangpoti
PY - 2026
DA - 2026/05/30
JO - Journal of Natural Pesticide Research
DO - 10.1016/j.napere.2026.100201
UR - https://hal.science/hal-05783420v1
AB - The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a highly destructive and polyphagous pest that has developed resistance to several classes of synthetic insecticides. This study evaluated the insecticidal and antifeedant activities of Cyperus rotundus extracts against S. frugiperda larvae and aimed to identify bioactive compounds with potential for development as botanical insecticides. Among the tested solvent fractions, the dichloromethane extract (LD₅₀ = 4.58 μg/larva) and the isolated compound cadalene (LD₅₀ = 4.12 μg/larva) exhibited insecticidal activity against S. frugiperda larvae. The positive control cypermethrin showed an LD₅₀ value of 0.01 μg/larva, indicating that cadalene exhibited notable toxicity relative to the conventional insecticide. Enzyme assays revealed significant inhibition of carboxylesterase and glutathione Stransferase activities, suggesting interference with detoxification pathways. Chromatographic analysis identified cadalene as the bioactive compound. In feeding assays, the hexane extract exhibited the strongest antifeedant activity, with an FI₅₀ value of 17.13 µg/cm² at 2 h post-treatment. Larvae feeding on treated diets showed delayed development and increased mortality. These findings highlight the potential of compounds derived from C. rotundus as promising plant-based candidates for the development of botanical insecticides for sustainable pest management.
SN - 2773-0786
ER -