Adjunctive Autologous Platelet-Rich Plasma Activated with Autologous Thrombin Serum for a Refractory Below-Knee Amputation Stump Wound: A Case Report.
4 affiliations
- 1Nursing Department, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia.
- 2Surgery Department, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia.
- 3Research Office, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia.
- 4College of Medicine, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
Abstract
Background and Clinical Significance: Refractory below-knee amputation (BKA) stump wounds can delay prosthetic rehabilitation. This report describes wound healing during multimodal care that included autologous platelet-rich plasma (PRP) activated with autologous thrombin serum (ATS); Case Presentation: A 33-year-old man with Charcot-Marie-Tooth disease and a persistent right BKA stump wound after surgical revisions underwent three PRP-ATS applications on 16 and 23 February and 8 March 2026. Collagen dressings were applied on 2 and 15 March, and compression was continued thereafter. The estimated wound volume decreased from 14.18 cm³ at baseline to 0.48 cm³ at week 3 (approximately 97%); advanced epithelialization was observed at week 4. Complete closure was documented on 16 April 2026, approximately eight weeks after treatment began. No treatment-related adverse events were reported. After closure, the patient was referred for prosthetic rehabilitation assessment; fitting and functional use were not documented; Conclusions: Healing was temporally associated with PRP-ATS as part of a multimodal treatment course. This single case supports the feasibility of the approach but cannot establish the independent efficacy of PRP-ATS.
Related
- Autologous Platelet-Rich Plasma for Endometrial and Ovarian Dysfunction: Mechanistic Insights and Clinical Outcomes
- Autologous Peripheral Blood-Derived Stem Cells Combined with Platelet-Rich Plasma is Associated With Clinical and Radiological Improvement in Anterior Cruciate Ligament Ruptures—Preliminary Clinical and MRI Findings in 47 Consecutive Patients
- On closing the gap: anti-PD-1-based salvage in relapsed/ refractory classical Hodgkin lymphoma. Comment on: “Mind the gap: anti-PD-1 salvage before autologous transplantation in classical Hodgkin lymphoma”
- Associations of neutrophil-to-lymphocyte and platelet-to-lymphocyte ratios with amputation risk in patients with diabetic foot disease: a systematic review and meta-analysis
- Relapsed/refractory PBML complicated with failed platelet implantation and mixed infection after allo-HSCT : A case report
BibTeX
@article{shara2026adjunctive,
title = {{Adjunctive Autologous Platelet-Rich Plasma Activated with Autologous Thrombin Serum for a Refractory Below-Knee Amputation Stump Wound: A Case Report.}},
author = {Khansa Shara and Hasan AlAjmi and Nada Baatiah and Sara Darag and Renad Bakhamis and Amr Abdelhakim},
journal = {Reports (MDPI)},
year = {2026},
month = oct,
doi = {10.3390/reports9040351},
url = {https://europepmc.org/article/MED/42924803},
note = {CC BY},
}
RIS
TY - JOUR
TI - Adjunctive Autologous Platelet-Rich Plasma Activated with Autologous Thrombin Serum for a Refractory Below-Knee Amputation Stump Wound: A Case Report.
AU - Khansa Shara
AU - Hasan AlAjmi
AU - Nada Baatiah
AU - Sara Darag
AU - Renad Bakhamis
AU - Amr Abdelhakim
PY - 2026
DA - 2026/10/14
JO - Reports (MDPI)
DO - 10.3390/reports9040351
UR - https://europepmc.org/article/MED/42924803
AB - Background and Clinical Significance: Refractory below-knee amputation (BKA) stump wounds can delay prosthetic rehabilitation. This report describes wound healing during multimodal care that included autologous platelet-rich plasma (PRP) activated with autologous thrombin serum (ATS); Case Presentation: A 33-year-old man with Charcot-Marie-Tooth disease and a persistent right BKA stump wound after surgical revisions underwent three PRP-ATS applications on 16 and 23 February and 8 March 2026. Collagen dressings were applied on 2 and 15 March, and compression was continued thereafter. The estimated wound volume decreased from 14.18 cm³ at baseline to 0.48 cm³ at week 3 (approximately 97%); advanced epithelialization was observed at week 4. Complete closure was documented on 16 April 2026, approximately eight weeks after treatment began. No treatment-related adverse events were reported. After closure, the patient was referred for prosthetic rehabilitation assessment; fitting and functional use were not documented; Conclusions: Healing was temporally associated with PRP-ATS as part of a multimodal treatment course. This single case supports the feasibility of the approach but cannot establish the independent efficacy of PRP-ATS.
SN - 2571-841X
ER -