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Peer-reviewedCC BYData in brief

Bulk RNA sequencing dataset of hippocampi from RORγt transgenic mice and wild type littermates.

  • Tetsuya Sasaki1,2,3
  • Sae Sanaka1,4
  • Kenyu Nakamura1,4
  • Suguru Iwata1,3
  • Momo Morikawa1,3
  • Yosuke Takei1,2,3
4 affiliations
  1. 1Laboratory of Anatomy and Neuroscience, Department of Biomedical Sciences, Institute of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Ibaraki, Japan.
  2. 2Master's Program in Frontier Medical Sciences, Degree Program of Comprehensive Human Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Ibaraki, Japan.
  3. 3Master's and Doctoral Programs in Neuroscience, Degree Programs in Comprehensive Human Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Ibaraki, Japan.
  4. 4College of Medicine, School of Medicine and Health Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Ibaraki, Japan.
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Abstract

This dataset comprises bulk RNA-sequencing data from bilateral hippocampi of six untreated, 10-week-old male mice: three heterozygous RORγt-transgenic mice and three wild-type littermates on a C57BL/6 background. Total RNA was extracted using TRIzol, and RNA integrity numbers ranged from 8.1 to 8.6. Ribosomal RNA-depleted, directional libraries were prepared from 500 ng of RNA per sample and sequenced on an Illumina NextSeq 500 with a 2 × 36-bp paired-end configuration. The received reads were 35 or 36 bases long. The 48 original compressed FASTQ files were consolidated into 12 files, preserving 83,357,563 read pairs and their pairing. Original mm10 count and RPKM matrices each contain 49,585 feature entries. An additional GRCm39 reanalysis using Ensembl release 116 provides gene-level fragment counts for 78,348 annotated features, coordinate-sorted alignments and sample-level exploratory analyses. FastQC and MultiQC reports describe raw-read quality, and principal component analysis summarizes variation across all six samples. Original sequencing data and expression matrices are deposited in NCBI Gene Expression Omnibus under accession GSE347403.

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Cite

BibTeXRIS
BibTeX
@article{sasaki2026bulk,
  title = {{Bulk RNA sequencing dataset of hippocampi from RORγt transgenic mice and wild type littermates.}},
  author = {Tetsuya Sasaki and Sae Sanaka and Kenyu Nakamura and Suguru Iwata and Momo Morikawa and Yosuke Takei},
  journal = {Data in brief},
  year = {2026},
  month = aug,
  doi = {10.1016/j.dib.2026.113361},
  url = {https://europepmc.org/article/MED/42999513},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Bulk RNA sequencing dataset of hippocampi from RORγt transgenic mice and wild type littermates.
AU  - Tetsuya Sasaki
AU  - Sae Sanaka
AU  - Kenyu Nakamura
AU  - Suguru Iwata
AU  - Momo Morikawa
AU  - Yosuke Takei
PY  - 2026
DA  - 2026/08/01
JO  - Data in brief
DO  - 10.1016/j.dib.2026.113361
UR  - https://europepmc.org/article/MED/42999513
AB  - This dataset comprises bulk RNA-sequencing data from bilateral hippocampi of six untreated, 10-week-old male mice: three heterozygous RORγt-transgenic mice and three wild-type littermates on a C57BL/6 background. Total RNA was extracted using TRIzol, and RNA integrity numbers ranged from 8.1 to 8.6. Ribosomal RNA-depleted, directional libraries were prepared from 500 ng of RNA per sample and sequenced on an Illumina NextSeq 500 with a 2 × 36-bp paired-end configuration. The received reads were 35 or 36 bases long. The 48 original compressed FASTQ files were consolidated into 12 files, preserving 83,357,563 read pairs and their pairing. Original mm10 count and RPKM matrices each contain 49,585 feature entries. An additional GRCm39 reanalysis using Ensembl release 116 provides gene-level fragment counts for 78,348 annotated features, coordinate-sorted alignments and sample-level exploratory analyses. FastQC and MultiQC reports describe raw-read quality, and principal component analysis summarizes variation across all six samples. Original sequencing data and expression matrices are deposited in NCBI Gene Expression Omnibus under accession GSE347403.
SN  - 2352-3409
ER  -