Type I interferon upregulates IL-18RAP in neutrophils to enhance chemotaxis and bactericidal activity in autoimmune liver diseases.
4 affiliations
- 1Department of Clinical Laboratory, Hunan Provincial People's Hospital, Department of Pathology, QiluHospital of Shandong University Dezhou Hospital, Dezhou, China.
- 2Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, No. 61, Jiefang West Road, Changsha, Hunan Province, China, Changsha, 410000, China.
- 3Department of Clinical Laboratory, The Affiliated Huizhou Hospital, Guangzhou Medical University, Huizhou, China.
- 4Changsha County People's Hospital (Xingsha Hospital District of Hunan Provincial People's Hospital), Changsha, China.
Abstract
Objective
Given the established association of interleukin-18 (IL-18) and IL-18 receptor accessory protein (IL-18RAP) with autoimmune liver diseases (AILD), while their specific mechanism remains unclear, this study aimed to investigate the expression and functional significance of IL-18RAP in neutrophils from patients with AILD.Methods
The expression levels of IL-18RAP were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Serum type I interferon (IFN) concentrations were measured by enzyme-linked immunosorbent assay (ELISA). In vitro cell experiments were conducted to assess chemotactic phagocytosis and to analyze the regulatory effect of IL-18RAP on neutrophil function. In vitro IFN stimulation experiments were performed to analyze the relationship between IL-18RAP expression and type I IFN.Results
IL-18RAP mRNA and protein expression levels were increased in patients with AILD. After IL-18 stimulation, the chemotaxis index and nitroblue tetrazolium (NBT) reduction rate of neutrophils in the AILD group increased (P < 0.05). Serum type I IFN concentrations were elevated in AILD patients (Z = -2.14, P = 0.032). IL-18RAP mRNA expression was upregulated in the group stimulated with high-concentration patient serum and in the human recombinant IFN-α stimulation group, indicating that high concentrations of type I IFN can upregulate IL-18RAP expression in neutrophils.Conclusion
Elevated IL-18RAP expression in AILD patients enhanced the chemotactic, phagocytic, and bactericidal functions of neutrophils. Higher serum type I IFN levels in AILD patients upregulated IL-18RAP expression in neutrophils, suggesting that type I IFN contributes to the pathogenesis of AILD by upregulating IL-18RAP.
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BibTeX
@article{jin2026type,
title = {{Type I interferon upregulates IL-18RAP in neutrophils to enhance chemotaxis and bactericidal activity in autoimmune liver diseases.}},
author = {Wenwen Jin and Xi Su and Lizhi Liu and Shilin Du and Wenting Xiong and Zifei Zhu and Cunyan Li},
journal = {Journal of translational autoimmunity},
year = {2026},
month = oct,
doi = {10.1016/j.jtauto.2026.100406},
url = {https://europepmc.org/article/MED/42898347},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Type I interferon upregulates IL-18RAP in neutrophils to enhance chemotaxis and bactericidal activity in autoimmune liver diseases.
AU - Wenwen Jin
AU - Xi Su
AU - Lizhi Liu
AU - Shilin Du
AU - Wenting Xiong
AU - Zifei Zhu
AU - Cunyan Li
PY - 2026
DA - 2026/10/01
JO - Journal of translational autoimmunity
DO - 10.1016/j.jtauto.2026.100406
UR - https://europepmc.org/article/MED/42898347
AB - Objective Given the established association of interleukin-18 (IL-18) and IL-18 receptor accessory protein (IL-18RAP) with autoimmune liver diseases (AILD), while their specific mechanism remains unclear, this study aimed to investigate the expression and functional significance of IL-18RAP in neutrophils from patients with AILD.Methods The expression levels of IL-18RAP were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Serum type I interferon (IFN) concentrations were measured by enzyme-linked immunosorbent assay (ELISA). In vitro cell experiments were conducted to assess chemotactic phagocytosis and to analyze the regulatory effect of IL-18RAP on neutrophil function. In vitro IFN stimulation experiments were performed to analyze the relationship between IL-18RAP expression and type I IFN.Results IL-18RAP mRNA and protein expression levels were increased in patients with AILD. After IL-18 stimulation, the chemotaxis index and nitroblue tetrazolium (NBT) reduction rate of neutrophils in the AILD group increased (P < 0.05). Serum type I IFN concentrations were elevated in AILD patients (Z = -2.14, P = 0.032). IL-18RAP mRNA expression was upregulated in the group stimulated with high-concentration patient serum and in the human recombinant IFN-α stimulation group, indicating that high concentrations of type I IFN can upregulate IL-18RAP expression in neutrophils.Conclusion Elevated IL-18RAP expression in AILD patients enhanced the chemotactic, phagocytic, and bactericidal functions of neutrophils. Higher serum type I IFN levels in AILD patients upregulated IL-18RAP expression in neutrophils, suggesting that type I IFN contributes to the pathogenesis of AILD by upregulating IL-18RAP.
SN - 2589-9090
ER -