Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
- 1Department of Plastic, Aesthetic, Hand and Reconstructive Surgery, Hanover Medical School, Carl-Neuberg-Strasse 1, Hannover, D 30625, Germany.
Abstract
Lifeguard (LFG/FAIM2) is an anti-apoptotic transmembrane protein implicated in apoptosis resistance and breast cancer progression. However, the molecular mechanisms underlying its biological function remain incompletely understood. In the present study, we investigated potential interactions between LFG and phosphoinositides in the breast cancer cell line MDA-MB-231. Using membrane lipid strip and phosphoinositide (PIP) array assays, we observed binding signals between LFG and several phosphoinositide species, indicating potential lipid-binding preferences under the experimental conditions employed. Because phosphoinositides participate in intracellular calcium signaling pathways, we next examined whether reduced LFG expression was associated with alterations in intracellular calcium homeostasis. Using a fluorescence-based calcium assay, we found that siRNA-mediated downregulation of LFG was associated with increased intracellular calcium-associated fluorescence. Overall, our exploratory findings suggest that LFG may participate in phosphoinositide-associated signaling and intracellular calcium homeostasis in the breast cancer cell model examined. Further studies will be required to validate these observations, determine the underlying molecular mechanisms, and assess their potential relevance for apoptosis regulation and breast cancer progression.
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BibTeX
@article{welzel2026interaction,
title = {{Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.}},
author = {Anna Marie Welzel and Lena Maria Herrmann and Nadjib Dastagir and Sarah Strauß and Peter M Vogt and Vesna Bucan},
journal = {Biochemistry and biophysics reports},
year = {2026},
month = oct,
doi = {10.1016/j.bbrep.2026.102830},
url = {https://europepmc.org/article/MED/42898335},
note = {CC BY},
}
RIS
TY - JOUR
TI - Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
AU - Anna Marie Welzel
AU - Lena Maria Herrmann
AU - Nadjib Dastagir
AU - Sarah Strauß
AU - Peter M Vogt
AU - Vesna Bucan
PY - 2026
DA - 2026/10/09
JO - Biochemistry and biophysics reports
DO - 10.1016/j.bbrep.2026.102830
UR - https://europepmc.org/article/MED/42898335
AB - Lifeguard (LFG/FAIM2) is an anti-apoptotic transmembrane protein implicated in apoptosis resistance and breast cancer progression. However, the molecular mechanisms underlying its biological function remain incompletely understood. In the present study, we investigated potential interactions between LFG and phosphoinositides in the breast cancer cell line MDA-MB-231. Using membrane lipid strip and phosphoinositide (PIP) array assays, we observed binding signals between LFG and several phosphoinositide species, indicating potential lipid-binding preferences under the experimental conditions employed. Because phosphoinositides participate in intracellular calcium signaling pathways, we next examined whether reduced LFG expression was associated with alterations in intracellular calcium homeostasis. Using a fluorescence-based calcium assay, we found that siRNA-mediated downregulation of LFG was associated with increased intracellular calcium-associated fluorescence. Overall, our exploratory findings suggest that LFG may participate in phosphoinositide-associated signaling and intracellular calcium homeostasis in the breast cancer cell model examined. Further studies will be required to validate these observations, determine the underlying molecular mechanisms, and assess their potential relevance for apoptosis regulation and breast cancer progression.
SN - 2405-5808
ER -