Collaborative studies on in vitro digestibility of dietary proteins based on the INFOGEST Quant protocol: toward international standardization

@article{egger2026collaborative,
  title = {{Collaborative studies on in vitro digestibility of dietary proteins based on the INFOGEST Quant protocol: toward international standardization}},
  author = {Lotti Egger and Ana Blanco-Doval and Cédric Brügger and Raquel Sousa and Lychou Abbühl and Marta Santos Hernández and Beatriz Miralles and Cristina Gómez-Marin and Adam Macierzanka and Patrycja Szumała and Ilona Kłosowska-Chomiczewska and Ana Isabel Mulet-Cabero and Natalia Perez-Moral and Cathrina Edwards and Shannon Gwala and Conor Fitzpatrick and Anne Rieder and Uri Lesmes and Leehen Mashiah and Carmit Shani-Levi and Catarina Gonçalves and Isabel Rodriguez Amado and Clarisse Nobre and Ana Pinheiro and Elena Arranz and Francesca Bot and James O'Mahony and Myriam Grundy and Frédérique Mayeur-Nickel and Igor Pacheco Cruz and Ailynne Sepúlveda-González and Elva Gonzales-Nieto and Irene Comi and László Abrankó and Judit Tormási and Felipe González-González and Patricia Ruas-Madiedo and Lorena Ruiz and Miguel Ângelo Faria and Helena Ramos and Luis Jiménez-Munoz and Milena Corredig and Molly Muleya and Natalia Rosa-Sibakov and Kerensa Broersen and Soenita Goerdayal and Olivia Ménard and Marie-Françoise Cochet and Gwénaële Henry and Vibeke Orlien and Ourania Gouseti and Iben Lykke Petersen and Patricia Duque-Estrada and Raquel Olias and Cristina Delgado-Andrade and Sebnem Simsek and Sedef Nehir El and Sibel Karakaya and Serge Rezzi and Stéphane Canarelli},
  journal = {Food Research International},
  year = {2026},
  month = sep,
  doi = {10.1016/j.foodres.2026.120598},
  url = {https://hal.inrae.fr/hal-05764059v1},
  note = {CC BY},
}

TY  - JOUR
TI  - Collaborative studies on in vitro digestibility of dietary proteins based on the INFOGEST Quant protocol: toward international standardization
AU  - Lotti Egger
AU  - Ana Blanco-Doval
AU  - Cédric Brügger
AU  - Raquel Sousa
AU  - Lychou Abbühl
AU  - Marta Santos Hernández
AU  - Beatriz Miralles
AU  - Cristina Gómez-Marin
AU  - Adam Macierzanka
AU  - Patrycja Szumała
AU  - Ilona Kłosowska-Chomiczewska
AU  - Ana Isabel Mulet-Cabero
AU  - Natalia Perez-Moral
AU  - Cathrina Edwards
AU  - Shannon Gwala
AU  - Conor Fitzpatrick
AU  - Anne Rieder
AU  - Uri Lesmes
AU  - Leehen Mashiah
AU  - Carmit Shani-Levi
AU  - Catarina Gonçalves
AU  - Isabel Rodriguez Amado
AU  - Clarisse Nobre
AU  - Ana Pinheiro
AU  - Elena Arranz
AU  - Francesca Bot
AU  - James O'Mahony
AU  - Myriam Grundy
AU  - Frédérique Mayeur-Nickel
AU  - Igor Pacheco Cruz
AU  - Ailynne Sepúlveda-González
AU  - Elva Gonzales-Nieto
AU  - Irene Comi
AU  - László Abrankó
AU  - Judit Tormási
AU  - Felipe González-González
AU  - Patricia Ruas-Madiedo
AU  - Lorena Ruiz
AU  - Miguel Ângelo Faria
AU  - Helena Ramos
AU  - Luis Jiménez-Munoz
AU  - Milena Corredig
AU  - Molly Muleya
AU  - Natalia Rosa-Sibakov
AU  - Kerensa Broersen
AU  - Soenita Goerdayal
AU  - Olivia Ménard
AU  - Marie-Françoise Cochet
AU  - Gwénaële Henry
AU  - Vibeke Orlien
AU  - Ourania Gouseti
AU  - Iben Lykke Petersen
AU  - Patricia Duque-Estrada
AU  - Raquel Olias
AU  - Cristina Delgado-Andrade
AU  - Sebnem Simsek
AU  - Sedef Nehir El
AU  - Sibel Karakaya
AU  - Serge Rezzi
AU  - Stéphane Canarelli
PY  - 2026
DA  - 2026/09/25
JO  - Food Research International
DO  - 10.1016/j.foodres.2026.120598
UR  - https://hal.inrae.fr/hal-05764059v1
AB  - Based on recommendations by the Food and Agriculture Organization, an international standardization was launched for a static in vitro protein digestibility method, recently published as the INFOGEST Quant protocol. Based on the validated INFOGEST 2.0 method, the INFOGEST Quant protocol allows quantification of total protein digestibility, individual amino acid digestibility, and digestible indispensable amino acid score (DIAAS) calculation. Comparability of protein digestibility obtained with the INFOGEST Quant method was confirmed using identical substrates in vitro and in vivo. Method standardization within the International Dairy Federation and the International Organization for Standardization was initiated, and precision data were established in two international collaborative studies by over 25 laboratories. The three applied analytical endpoints were comparable. Robust precision estimates for o-phthalaldehyde and total nitrogen showed repeatability and reproducibility limits of 10% and 15%, respectively. Moreover, the protein digestibility of fresh dairy foods was higher than in freeze-dried samples, highlighting matrix and processing effects.
SN  - 0963-9969
ER  -