Dual roles of AXIN1 as a β-catenin p-S60-dependent cytokinesis machinery and an ESCRT component
Abstract
AXIN1 functions as a scaffolding protein in several signaling pathways, including TGF-β, SAPK/JNK, and Wnt; however, as there have been no reports on the function of AXIN1 in cytokinesis, we investigated this link to determine if this scaffold coordinates cell division. Here, we demonstrate that AXIN1 is located in the central dark zone of the intercellular bridge, known as the Flemming body, where it forms a midbody ring structure. We also demonstrated that AXIN1 functions in coordination with β-catenin p-S60-dependent cytokinetic machinery during early telophase. Furthermore, AXIN1 regulates the hierarchy of the ESCRT complex by positioning itself between MKLP1 and CEP55 in late telophase cells. Based on our findings, we propose that AXIN1 has dual functions in cytokinesis by facilitating ECT2 recruitment during early telophase and promoting recruitment of abscission components during late telophase, highlighting the transformative role of this signaling scaffold as structural machinery in coordinating cell division.
Related
- Functional Roles of Wnt/β-catenin Pathway Components in Pre-Basal Cell Carcinoma-like Cell Proliferation and Survival
- Phosphorylation-dependent binding of the Adenomatous Polyposis Coli protein to β-TrCP1 regulates β-catenin destruction
- Inositol-Dependent Alleviation of Lithium-Induced Embryonic Neurotoxicity in Mice Through the GSK3β/β-Catenin/CyclinD1 Pathway
- Heme-Mediated Danger Signaling Suppresses Osteogenesis Through TLR4-Dependent Inhibition of β-Catenin Signaling
- One ring to rule: Cytokinesis in model and pathogenic fungi
BibTeX
@article{lee2026dual,
title = {{Dual roles of AXIN1 as a β-catenin p-S60-dependent cytokinesis machinery and an ESCRT component}},
author = {Na Gyeong Lee and Na Yeong Lee and Mi-Lang Kyun and Ji Eun Yu and Sun-Ok Kim and Yoon-Hee Na and Taeho Kwon and Hee Jun Cho and Key-Hwan Lim and Kyung Ho Lee},
journal = {iScience},
year = {2026},
month = oct,
doi = {10.1016/j.isci.2026.117923},
url = {https://www.cell.com/iscience/fulltext/S2589-0042(26)03306-7},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Dual roles of AXIN1 as a β-catenin p-S60-dependent cytokinesis machinery and an ESCRT component
AU - Na Gyeong Lee
AU - Na Yeong Lee
AU - Mi-Lang Kyun
AU - Ji Eun Yu
AU - Sun-Ok Kim
AU - Yoon-Hee Na
AU - Taeho Kwon
AU - Hee Jun Cho
AU - Key-Hwan Lim
AU - Kyung Ho Lee
PY - 2026
DA - 2026/10/16
JO - iScience
DO - 10.1016/j.isci.2026.117923
UR - https://www.cell.com/iscience/fulltext/S2589-0042(26)03306-7
AB - AXIN1 functions as a scaffolding protein in several signaling pathways, including TGF-β, SAPK/JNK, and Wnt; however, as there have been no reports on the function of AXIN1 in cytokinesis, we investigated this link to determine if this scaffold coordinates cell division. Here, we demonstrate that AXIN1 is located in the central dark zone of the intercellular bridge, known as the Flemming body, where it forms a midbody ring structure. We also demonstrated that AXIN1 functions in coordination with β-catenin p-S60-dependent cytokinetic machinery during early telophase. Furthermore, AXIN1 regulates the hierarchy of the ESCRT complex by positioning itself between MKLP1 and CEP55 in late telophase cells. Based on our findings, we propose that AXIN1 has dual functions in cytokinesis by facilitating ECT2 recruitment during early telophase and promoting recruitment of abscission components during late telophase, highlighting the transformative role of this signaling scaffold as structural machinery in coordinating cell division.
SN - 2589-0042
ER -