Comparative biochemical analysis of tea (Camellia sinensis) and coffee (Coffea arabica) extracts identifies the ethyl acetate fraction of green tea as a potential dual digestive enzyme inhibitor: an in vitro and in silico study.
4 affiliations
- 1Biochemistry Division, Chemistry Department, Mansoura University Mansoura 35516 Egypt [email protected].
- 2Department of Pharmacognosy, Faculty of Pharmacy, Kafrelsheikh University Mansoura 33516 Egypt [email protected].
- 3Unit of Drug Analysis, Faculty of Pharmacy, Mansoura University 35516 Egypt.
- 4Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University Mansoura 35516 Egypt [email protected].
Abstract
Metabolic diseases, including obesity, type 2 diabetes mellitus, and non-alcoholic fatty liver disease (NAFLD), have prompted interest in identifying natural products capable of modulating digestive enzymes. This study comparatively evaluated the phytochemical composition, antioxidant activity, and digestive enzyme inhibitory activities of green tea (GT), black tea (BT), and green coffee (GC) extracts. Phytochemical screening, antioxidant assays (DPPH, ABTS, and SOD-like), and quantitative phenolic analysis indicated that GT contained the highest phenolic content and exhibited the greatest antioxidant activity, whereas GC showed the highest alkaloid content. Among the fractionated extracts, the ethyl acetate fraction of green tea (EtOAc fraction of GT) was selected for further investigation based on its phenolic content, pancreatic lipase inhibitory activity, and overall biological profile. HPLC analysis identified ferulic acid and chlorogenic acid as major constituents, together with syringic acid, resveratrol, myricetin, and rosmarinic acid. Both the crude BT extract and EtOAc fraction of GT exhibited α-amylase inhibitory activity comparable to that of quercetin. Kinetic analysis indicated competitive inhibition by the EtOAc fraction of GT (IC₅₀ = 0.662 mg mL⁻¹), whereas BT displayed mixed-type inhibition with a strong uncompetitive component. The EtOAc fraction of GT also showed the lowest IC₅₀ against pancreatic lipase (0.264 mg mL⁻¹) and followed a non-competitive inhibition mechanism. The EtOAc fraction maintained 98.94% of human skin fibroblast viability after 48 h at 500 µg mL⁻¹ in a preliminary cytotoxicity screen. Molecular docking was consistent with the experimental findings, suggesting that hesperidin and rutin may contribute to competitive α-amylase inhibition and allosteric pancreatic lipase modulation. These findings indicate that the ethyl acetate fraction of green tea is a phenolic-rich fraction with dual digestive enzyme inhibitory activity that merits further evaluation in appropriate biological models.
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BibTeX
@article{elsaied2026comparative,
title = {{Comparative biochemical analysis of tea (Camellia sinensis) and coffee (Coffea arabica) extracts identifies the ethyl acetate fraction of green tea as a potential dual digestive enzyme inhibitor: an in vitro and in silico study.}},
author = {Youmna E El-Saied and Abdullah A Elgazar and Fardous F El-Senduny and Nada E Hammouda and Omali Y El-Khawaga and Farid A Badria},
journal = {RSC advances},
year = {2026},
month = oct,
doi = {10.1039/d6ra07857e},
url = {https://europepmc.org/article/MED/42899091},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Comparative biochemical analysis of tea (Camellia sinensis) and coffee (Coffea arabica) extracts identifies the ethyl acetate fraction of green tea as a potential dual digestive enzyme inhibitor: an in vitro and in silico study.
AU - Youmna E El-Saied
AU - Abdullah A Elgazar
AU - Fardous F El-Senduny
AU - Nada E Hammouda
AU - Omali Y El-Khawaga
AU - Farid A Badria
PY - 2026
DA - 2026/10/19
JO - RSC advances
DO - 10.1039/d6ra07857e
UR - https://europepmc.org/article/MED/42899091
AB - Metabolic diseases, including obesity, type 2 diabetes mellitus, and non-alcoholic fatty liver disease (NAFLD), have prompted interest in identifying natural products capable of modulating digestive enzymes. This study comparatively evaluated the phytochemical composition, antioxidant activity, and digestive enzyme inhibitory activities of green tea (GT), black tea (BT), and green coffee (GC) extracts. Phytochemical screening, antioxidant assays (DPPH, ABTS, and SOD-like), and quantitative phenolic analysis indicated that GT contained the highest phenolic content and exhibited the greatest antioxidant activity, whereas GC showed the highest alkaloid content. Among the fractionated extracts, the ethyl acetate fraction of green tea (EtOAc fraction of GT) was selected for further investigation based on its phenolic content, pancreatic lipase inhibitory activity, and overall biological profile. HPLC analysis identified ferulic acid and chlorogenic acid as major constituents, together with syringic acid, resveratrol, myricetin, and rosmarinic acid. Both the crude BT extract and EtOAc fraction of GT exhibited α-amylase inhibitory activity comparable to that of quercetin. Kinetic analysis indicated competitive inhibition by the EtOAc fraction of GT (IC₅₀ = 0.662 mg mL⁻¹), whereas BT displayed mixed-type inhibition with a strong uncompetitive component. The EtOAc fraction of GT also showed the lowest IC₅₀ against pancreatic lipase (0.264 mg mL⁻¹) and followed a non-competitive inhibition mechanism. The EtOAc fraction maintained 98.94% of human skin fibroblast viability after 48 h at 500 µg mL⁻¹ in a preliminary cytotoxicity screen. Molecular docking was consistent with the experimental findings, suggesting that hesperidin and rutin may contribute to competitive α-amylase inhibition and allosteric pancreatic lipase modulation. These findings indicate that the ethyl acetate fraction of green tea is a phenolic-rich fraction with dual digestive enzyme inhibitory activity that merits further evaluation in appropriate biological models.
SN - 2046-2069
ER -