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Peer-reviewedCC BY-NC-NDNature communications

Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells.

  • Laura J Sherwood1
  • Ronel Z Samuel2
  • Sai Harsha Bhamidipati2
  • Kihoon Nam3,4
  • Frank Maslow3,4
  • Travis Small3,4
  • Yali Zhang5
  • Jianmin Wang5
  • Song Liu5
  • Olga J Baker3,4,6
  • Stelios T Andreadis7,8,9,10
10 affiliations
  1. 1Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA.
  2. 2Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA.
  3. 3Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.
  4. 4Department of Otolaryngology-Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, MO, USA.
  5. 5Department of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
  6. 6Department of Biochemistry, University of Missouri, Columbia, MO, USA.
  7. 7Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA. [email protected].
  8. 8Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA. [email protected].
  9. 9New York State Center of Excellence in Bioinformatics and Life Sciences, Buffalo, NY, USA. [email protected].
  10. 10Center of Cell, Gene and Tissue Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA. [email protected].
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Abstract

Salivary gland (SG) hypofunction significantly affects the life quality of patients with hyposalivation. Current treatment options for these conditions are limited and often result in undesirable side effects. To address this issue, we've developed a developmentally inspired strategy to derive salivary gland epithelial progenitor (SGEP) cells from human pluripotent stem cells (PSC). Then we employ SGEP cells derived from human PSC with a SOX10::GFP reporter to engineer three-dimensional lumenized SG organoids, with well-defined lumens and secretory function, reminiscent of human salivary glands. Upon transplantation into the submandibular gland of athymic nude mice, mCherry+ salivary gland organoids (SGO) integrate into the gland and differentiated into salivary gland phenotypes, including acinar, ductal and myoepithelial structures. Our findings suggest that SG organoids may hold promise for studying SG development, disease progression and drug screening, as well as for development of cell therapies for salivary gland regeneration.

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Cite

BibTeXRIS
BibTeX
@article{sherwood2026developmentinspired,
  title = {{Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells.}},
  author = {Laura J Sherwood and Ronel Z Samuel and Sai Harsha Bhamidipati and Kihoon Nam and Frank Maslow and Travis Small and Yali Zhang and Jianmin Wang and Song Liu and Olga J Baker and Stelios T Andreadis},
  journal = {Nature communications},
  year = {2026},
  month = sep,
  doi = {10.1038/s41467-026-77724-5},
  url = {https://europepmc.org/article/MED/42897156},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells.
AU  - Laura J Sherwood
AU  - Ronel Z Samuel
AU  - Sai Harsha Bhamidipati
AU  - Kihoon Nam
AU  - Frank Maslow
AU  - Travis Small
AU  - Yali Zhang
AU  - Jianmin Wang
AU  - Song Liu
AU  - Olga J Baker
AU  - Stelios T Andreadis
PY  - 2026
DA  - 2026/09/17
JO  - Nature communications
DO  - 10.1038/s41467-026-77724-5
UR  - https://europepmc.org/article/MED/42897156
AB  - Salivary gland (SG) hypofunction significantly affects the life quality of patients with hyposalivation. Current treatment options for these conditions are limited and often result in undesirable side effects. To address this issue, we've developed a developmentally inspired strategy to derive salivary gland epithelial progenitor (SGEP) cells from human pluripotent stem cells (PSC). Then we employ SGEP cells derived from human PSC with a SOX10::GFP reporter to engineer three-dimensional lumenized SG organoids, with well-defined lumens and secretory function, reminiscent of human salivary glands. Upon transplantation into the submandibular gland of athymic nude mice, mCherry+ salivary gland organoids (SGO) integrate into the gland and differentiated into salivary gland phenotypes, including acinar, ductal and myoepithelial structures. Our findings suggest that SG organoids may hold promise for studying SG development, disease progression and drug screening, as well as for development of cell therapies for salivary gland regeneration.
SN  - 2041-1723
ER  -