Reverse vaccinology for development of vaccine against Aeromonas hydrophila infection in nile tilapia (Oreochromis niloticus).
11 affiliations
- 1Center for Open Access Genomic Analysis - CALAnGO, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, CEP 54.300-300, Petrolina, Pernambuco, Brazil.
- 2Programa de Pós Graduação em Ciências Veterinárias no Semiárido. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco, Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil.
- 3Federal Institute (Instituto Federal - IF), Maria Luzia de Araújo Gomes Cabral, 791 - João de Deus, Petrolina, PE, CEP 56309-680, Brazil.
- 4Programa de Pós Graduação em Ciência Animal. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil.
- 5Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
- 6Microbiology and Parasitology, Institute of Biological Sciences and Natural Sciences, Universidade Federal do Triângulo Mineiro, Uberaba, Brazil.
- 7Universidade Federal da Bahia, Salvador, BA, CEP 40170-110, Brazil.
- 8Dept. of Molecular Biology of Livestock, Georg August University Göttingen, Burckhardtweg 2, Göttingen, 37077, Germany.
- 9Center for Open Access Genomic Analysis - CALAnGO, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, CEP 54.300-300, Petrolina, Pernambuco, Brazil. [email protected].
- 10Programa de Pós Graduação em Ciências Veterinárias no Semiárido. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco, Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil. [email protected].
- 11Programa de Pós Graduação em Ciência Animal. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil. [email protected].
Abstract
Aeromonas hydrophila is one of the main pathogens of Tilapia and has a wide range phenotype, making it difficult to prevent by a unique vaccine. And turning the majority of the treatment using antibiotics. The aim of this work was to predict vaccine candidate with potential efficacy to protect Oreochromis niloticus against Aeromonas hydrophila, applying bioinformatics tools in a reverse vaccinology approach. It was obtained 35 A. hydrophila genomes in the São Francisco Valley in Petrolina/PE/Brazil, as well as 24 A. hydrophila and 02 O. niloticus complete genomes available in Genbank. After identification of core genes of Aeromonas hydrophila and a subtractive genomics analysis between the O. niloticus genome and A. hydrophila genome, 32 of the 178 resulting proteins, were considered likely vaccine targets as they showed a high adherence probability (> 0.51) in the Vaxign tool. After BLAST analysis against the database of essential genes (DEG), 18 proteins showed defined criteria e-value (≤ 10- 6) and a relevant similarity with the proteins of pathogenic species from the genus Aeromonas. Were selected 10 proteins, based on the criteria of portability adhesion, molecular mass between 19 and 80 kDa and presence of one or no transmembrane helix. The analyses identified nine proteins [secreted (n = 8) and PSE (n = 1)] with immunological and novel properties as candidates for vaccine targets. Finally, in vitro/in vivo tests will be necessary to experimentally validate the results of this search.
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BibTeX
@article{carvalho2026reverse,
title = {{Reverse vaccinology for development of vaccine against Aeromonas hydrophila infection in nile tilapia (Oreochromis niloticus).}},
author = {Germana Karla de Lima Carvalho and Leticia Stheffany de Barros Cunha and Maria Luiza Evangelista de Brito and Ligia Carolina da Silva Prado and Siomar de Castro Soares and Vasco Ariston de Carvalho Azevedo and Bertram Brenig and Mateus Matiuzzi da Costa and João José de Simoni Gouveia and Gisele Veneroni Gouveia and Gisele Veneroni Gouveia},
journal = {Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]},
year = {2026},
month = oct,
doi = {10.1007/s42770-026-02127-z},
url = {https://europepmc.org/article/MED/42911516},
note = {CC BY},
}
RIS
TY - JOUR
TI - Reverse vaccinology for development of vaccine against Aeromonas hydrophila infection in nile tilapia (Oreochromis niloticus).
AU - Germana Karla de Lima Carvalho
AU - Leticia Stheffany de Barros Cunha
AU - Maria Luiza Evangelista de Brito
AU - Ligia Carolina da Silva Prado
AU - Siomar de Castro Soares
AU - Vasco Ariston de Carvalho Azevedo
AU - Bertram Brenig
AU - Mateus Matiuzzi da Costa
AU - João José de Simoni Gouveia
AU - Gisele Veneroni Gouveia
AU - Gisele Veneroni Gouveia
PY - 2026
DA - 2026/10/22
JO - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
DO - 10.1007/s42770-026-02127-z
UR - https://europepmc.org/article/MED/42911516
AB - Aeromonas hydrophila is one of the main pathogens of Tilapia and has a wide range phenotype, making it difficult to prevent by a unique vaccine. And turning the majority of the treatment using antibiotics. The aim of this work was to predict vaccine candidate with potential efficacy to protect Oreochromis niloticus against Aeromonas hydrophila, applying bioinformatics tools in a reverse vaccinology approach. It was obtained 35 A. hydrophila genomes in the São Francisco Valley in Petrolina/PE/Brazil, as well as 24 A. hydrophila and 02 O. niloticus complete genomes available in Genbank. After identification of core genes of Aeromonas hydrophila and a subtractive genomics analysis between the O. niloticus genome and A. hydrophila genome, 32 of the 178 resulting proteins, were considered likely vaccine targets as they showed a high adherence probability (> 0.51) in the Vaxign tool. After BLAST analysis against the database of essential genes (DEG), 18 proteins showed defined criteria e-value (≤ 10- 6) and a relevant similarity with the proteins of pathogenic species from the genus Aeromonas. Were selected 10 proteins, based on the criteria of portability adhesion, molecular mass between 19 and 80 kDa and presence of one or no transmembrane helix. The analyses identified nine proteins [secreted (n = 8) and PSE (n = 1)] with immunological and novel properties as candidates for vaccine targets. Finally, in vitro/in vivo tests will be necessary to experimentally validate the results of this search.
SN - 1517-8382
SN - 1678-4405
ER -