Rhizospheric Bacteria From Artocarpus heterophyllus Exhibit Antimicrobial Traits and Antagonistic Activity Against Pantoea stewartii subsp. stewartii, Causal Agent of Jackfruit Bronzing Disease.
4 affiliations
- 1Plant-Microorganism Interaction Laboratory Office of Research and Graduate Studies Nayarit Center for Innovation and Technology Transfer (CENITT) Autonomous University of Nayarit Tepic Nayarit Mexico.
- 2Faculty of Professional Studies Huasteca Zone Autonomous University of San Luis Potosi Ciudad Valles San Luis Potosi Mexico uaslp.mx.
- 3Academic Unit of Chemical Biological and Pharmaceutical Sciences Autonomous University of Nayarit Tepic Nayarit Mexico.
- 4Faculty of Chemistry Autonomous University of Queretaro Santiago de Queretaro Queretaro Mexico uaq.mx.
Abstract
The Jackfruit bronzing disease represents a significant phytosanitary constraint affecting Artocarpus heterophyllus production. In this context, the exploration of plant-associated microorganisms as a source of potential biological control agents aligns with sustainable agricultural strategies. This study is aimed at isolating, characterizing, and evaluating the in vitro antagonistic interactions, and antimicrobial potential of indigenous rhizospheric and endophytic bacteria with the potential to be developed as biocontrol agents for the control jackfruit bronzing causal agent Pantoea stewartii subsp. stewartii. A total of 138 bacterial isolates were obtained from rhizospheric soils and internal plant tissues, with a predominance of rhizospheric bacteria (78.3%). In vitro antagonism assays revealed that only rhizospheric isolates exhibited inhibitory activity against the phytopathogen, identifying 12 strains with consistent antagonistic capacity. These isolates were further characterized to elucidate the potential mechanisms involved in microbial antagonism. Functional analyses demonstrated that the antagonistic isolates possessed multiple extracellular enzymatic activities, predominantly lipase, followed by amylase and cellulase activities, whereas protease activity was absent. Additionally, selected isolates exhibited biosurfactant production, suggesting their role in pathogen inhibition. Antibiosis was confirmed through the production of diffusible antimicrobial compounds. Furthermore, molecular screening revealed the presence of nonribosomal peptide synthetase (NRPS) gene fragments in several strains, supporting their genetic potential to synthesize bioactive secondary metabolites. Phylogenetic identification based on 16S rDNA gene sequencing assigned the isolates to bacterial taxa known for their ecological versatility and functional attributes. Overall, this study provides evidence that rhizospheric bacteria associated with jackfruit harbor significant antagonistic and antimicrobial capabilities against jackfruit bronzing causal agent. These findings contribute to the understanding of microbial interactions and functional traits associated with plant microbiota. This study provides the first assessment of microorganisms associated with jackfruit.
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BibTeX
@article{arvizugomez2026rhizospheric,
title = {{Rhizospheric Bacteria From Artocarpus heterophyllus Exhibit Antimicrobial Traits and Antagonistic Activity Against Pantoea stewartii subsp. stewartii, Causal Agent of Jackfruit Bronzing Disease.}},
author = {Jackeline Lizzeta Arvizu-Gómez and Alejandro Hernández-Morales and Christian González-Reyes and Cecilia Nheffreteri Cervantes-Díaz and Juan Campos-Guillén and Juan Ramiro Pacheco-Aguilar},
journal = {International journal of microbiology},
year = {2026},
month = oct,
doi = {10.1155/ijm/6036078},
url = {https://europepmc.org/article/MED/42928759},
note = {CC BY},
}
RIS
TY - JOUR
TI - Rhizospheric Bacteria From Artocarpus heterophyllus Exhibit Antimicrobial Traits and Antagonistic Activity Against Pantoea stewartii subsp. stewartii, Causal Agent of Jackfruit Bronzing Disease.
AU - Jackeline Lizzeta Arvizu-Gómez
AU - Alejandro Hernández-Morales
AU - Christian González-Reyes
AU - Cecilia Nheffreteri Cervantes-Díaz
AU - Juan Campos-Guillén
AU - Juan Ramiro Pacheco-Aguilar
PY - 2026
DA - 2026/10/25
JO - International journal of microbiology
DO - 10.1155/ijm/6036078
UR - https://europepmc.org/article/MED/42928759
AB - The Jackfruit bronzing disease represents a significant phytosanitary constraint affecting Artocarpus heterophyllus production. In this context, the exploration of plant-associated microorganisms as a source of potential biological control agents aligns with sustainable agricultural strategies. This study is aimed at isolating, characterizing, and evaluating the in vitro antagonistic interactions, and antimicrobial potential of indigenous rhizospheric and endophytic bacteria with the potential to be developed as biocontrol agents for the control jackfruit bronzing causal agent Pantoea stewartii subsp. stewartii. A total of 138 bacterial isolates were obtained from rhizospheric soils and internal plant tissues, with a predominance of rhizospheric bacteria (78.3%). In vitro antagonism assays revealed that only rhizospheric isolates exhibited inhibitory activity against the phytopathogen, identifying 12 strains with consistent antagonistic capacity. These isolates were further characterized to elucidate the potential mechanisms involved in microbial antagonism. Functional analyses demonstrated that the antagonistic isolates possessed multiple extracellular enzymatic activities, predominantly lipase, followed by amylase and cellulase activities, whereas protease activity was absent. Additionally, selected isolates exhibited biosurfactant production, suggesting their role in pathogen inhibition. Antibiosis was confirmed through the production of diffusible antimicrobial compounds. Furthermore, molecular screening revealed the presence of nonribosomal peptide synthetase (NRPS) gene fragments in several strains, supporting their genetic potential to synthesize bioactive secondary metabolites. Phylogenetic identification based on 16S rDNA gene sequencing assigned the isolates to bacterial taxa known for their ecological versatility and functional attributes. Overall, this study provides evidence that rhizospheric bacteria associated with jackfruit harbor significant antagonistic and antimicrobial capabilities against jackfruit bronzing causal agent. These findings contribute to the understanding of microbial interactions and functional traits associated with plant microbiota. This study provides the first assessment of microorganisms associated with jackfruit.
SN - 1687-918X
SN - 1687-9198
ER -