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Peer-reviewedCC BYNature Methods

Neuropixels Opto: combining high-resolution electrophysiology and optogenetics

  • Anna A. Lakunina1
  • Karolina Z. Socha2,3
  • Alexander Ladd4,5
  • Anna J. Bowen4,5
  • Susu Chen6
  • Jennifer I. Colonell6
  • Anjal Doshi1
  • Bill Karsh7,6
  • Michael Krumin2,3
  • Pavel Kulik1
  • Anna J. Li4,5
  • Pieter Neutens8
17 more authors
  • John M. O'Callaghan8
  • Meghan Olsen1
  • Jan Putzeys8
  • Charu Bai Reddy2,8
  • Harrie A. C. Tilmans8,5
  • Sara Vargas9,8
  • Marleen Welkenhuysen8,3
  • Zhiwen Ye4,10
  • Michael Häusser11,9
  • Christof Koch A10,8
  • Jonathan T. Ting9,4,6
  • Barundeb Dutta8,5
  • T.D. Harris7,6,1
  • Nicholas A. Steinmetz4,1
  • Karel Svoboda1,6,3
  • Joshua H. Siegle12
  • Matteo Carandini13
13 affiliations
  1. 1Allen Institute for Neural Dynamics, Seattle, WA, USA
  2. 2UCL Institute of Ophthalmology, University College London, London, UK
  3. 3University College London, London, UK
  4. 4Department of Neurobiology and Biophysics, University of Washington, Seattle, WA, USA
  5. 5University of Washington, Seattle, WA, USA
  6. 6Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
  7. 7Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA
  8. 8IMEC, Leuven, Belgium
  9. 9Allen Institute for Brain Science, Seattle, WA, USA
  10. 10Allen Institute MindScope Program, Seattle, WA, USA
  11. 11Wolfson Institute for Biomedical Research, University College London, London, UK
  12. 12Allen Institute for Neural Dynamics, Seattle, WA, USA. [email protected]
  13. 13UCL Institute of Ophthalmology, University College London, London, UK. [email protected]
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Abstract

High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations and is especially powerful when combined with optogenetics for manipulation of specific cell types with high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic and photonic circuits. These devices pack 960 electrical recording sites and two sets of 14 light emitters onto a 70-μm-wide, 1-cm-long shank, allowing spatially addressable optogenetic stimulation with blue and red light. In mouse cortex, Neuropixels Opto probes delivered high-quality recordings together with spatially addressable optogenetics, differentially activating or silencing neurons at distinct cortical depths. In the mouse striatum and other deep structures, Neuropixels Opto probes delivered efficient optotagging, facilitating the identification of two cell types in parallel. Neuropixels Opto probes represent a promising tool for recording, identifying and manipulating neuronal populations.

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Cite

BibTeXRIS
BibTeX
@article{lakunina2026neuropixels,
  title = {{Neuropixels Opto: combining high-resolution electrophysiology and optogenetics}},
  author = {Anna A. Lakunina and Karolina Z. Socha and Alexander Ladd and Anna J. Bowen and Susu Chen and Jennifer I. Colonell and Anjal Doshi and Bill Karsh and Michael Krumin and Pavel Kulik and Anna J. Li and Pieter Neutens and John M. O'Callaghan and Meghan Olsen and Jan Putzeys and Charu Bai Reddy and Harrie A. C. Tilmans and Sara Vargas and Marleen Welkenhuysen and Zhiwen Ye and Michael Häusser and Christof Koch A and Jonathan T. Ting and Barundeb Dutta and T.D. Harris and Nicholas A. Steinmetz and Karel Svoboda and Joshua H. Siegle and Matteo Carandini},
  journal = {Nature Methods},
  year = {2026},
  month = jun,
  doi = {10.1038/s41592-026-03076-z},
  url = {https://doi.org/10.1038/s41592-026-03076-z},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Neuropixels Opto: combining high-resolution electrophysiology and optogenetics
AU  - Anna A. Lakunina
AU  - Karolina Z. Socha
AU  - Alexander Ladd
AU  - Anna J. Bowen
AU  - Susu Chen
AU  - Jennifer I. Colonell
AU  - Anjal Doshi
AU  - Bill Karsh
AU  - Michael Krumin
AU  - Pavel Kulik
AU  - Anna J. Li
AU  - Pieter Neutens
AU  - John M. O'Callaghan
AU  - Meghan Olsen
AU  - Jan Putzeys
AU  - Charu Bai Reddy
AU  - Harrie A. C. Tilmans
AU  - Sara Vargas
AU  - Marleen Welkenhuysen
AU  - Zhiwen Ye
AU  - Michael Häusser
AU  - Christof Koch A
AU  - Jonathan T. Ting
AU  - Barundeb Dutta
AU  - T.D. Harris
AU  - Nicholas A. Steinmetz
AU  - Karel Svoboda
AU  - Joshua H. Siegle
AU  - Matteo Carandini
PY  - 2026
DA  - 2026/06/01
JO  - Nature Methods
DO  - 10.1038/s41592-026-03076-z
UR  - https://doi.org/10.1038/s41592-026-03076-z
AB  - High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations and is especially powerful when combined with optogenetics for manipulation of specific cell types with high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic and photonic circuits. These devices pack 960 electrical recording sites and two sets of 14 light emitters onto a 70-μm-wide, 1-cm-long shank, allowing spatially addressable optogenetic stimulation with blue and red light. In mouse cortex, Neuropixels Opto probes delivered high-quality recordings together with spatially addressable optogenetics, differentially activating or silencing neurons at distinct cortical depths. In the mouse striatum and other deep structures, Neuropixels Opto probes delivered efficient optotagging, facilitating the identification of two cell types in parallel. Neuropixels Opto probes represent a promising tool for recording, identifying and manipulating neuronal populations.
SN  - 1548-7091
SN  - 1548-7105
ER  -