Two Ume6 target gene classes in the biofilm regulatory network of Candida albicans
- 1University of Georgia
Abstract
Biofilm formation by the fungal pathogen Candida albicans is a major source of infection. The biofilm regulator Ume6 forms complexes with partner transcription factors Efg1, Ndt80, and Upc2 to drive expression of biofilm-related genes. Here we present chromatin immunoprecipitation with sequencing (ChIP-seq) data for Ume6-chromatin association in both wild-type and efg1{Delta}/{Delta} ndt80{Delta}/{Delta} upc2{Delta}/{Delta} backgrounds. Ume6 associates with two promoter region classes. For one class, Ume6 association is significantly decreased in the efg1{Delta}/{Delta} ndt80{Delta}/{Delta} upc2{Delta}/{Delta} background. This class includes 577 genes, many with well-established roles in biofilm formation or the related process of filamentation. For the second class, Ume6 association is unperturbed in the triple mutant background. This class includes 534 genes, some with roles in iron homeostasis (e.g., SFU1) and ergosterol synthesis (e.g., ERG11), which may contribute to the impact of Ume6 on ndt80{Delta}/{Delta} mutant azole drug sensitivity. It also includes FLO9, a putative adhesin gene that is required for the emergent biofilm state produced by efg1{Delta}/{Delta} ndt80{Delta}/{Delta} double mutants. The data show that Ume6 binding to ~half of its targets requires known partners, and Ume6 binding to ~half of its targets does not. Ume6 may bind to the latter set of promoter regions independently of any partner, or perhaps with additional partners that have yet to be discovered.
Related
- Integrated transcriptomic and network analysis identifies novel regulatory hubs driving biofilm formation in
- Candida albicans promotes self-adhesion and tumor cell progression of colorectal cancer cells.
- Proteomics to uncover new actors in the antifungal action of metformin. Impact on virulence traits, oxidative stress, and essential proteins from Candida albicans.
- Microbial Crosstalk in the Neonatal Gut: Candida albicans Impacts Group B Streptococcus Pathogenicity
- Chronological lifespan correlates with virulence within Candida albicans but long lifespan is not restricted to pathogenic Candida species
BibTeX
@misc{do2026ume6,
title = {{Two Ume6 target gene classes in the biofilm regulatory network of Candida albicans}},
author = {E. Do and J. McManus and A. P. Mitchell},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.24.753443},
url = {https://www.biorxiv.org/content/10.64898/2026.09.24.753443v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Two Ume6 target gene classes in the biofilm regulatory network of Candida albicans
AU - E. Do
AU - J. McManus
AU - A. P. Mitchell
PY - 2026
DA - 2026/09/24
PB - bioRxiv
DO - 10.64898/2026.09.24.753443
UR - https://www.biorxiv.org/content/10.64898/2026.09.24.753443v1
AB - Biofilm formation by the fungal pathogen Candida albicans is a major source of infection. The biofilm regulator Ume6 forms complexes with partner transcription factors Efg1, Ndt80, and Upc2 to drive expression of biofilm-related genes. Here we present chromatin immunoprecipitation with sequencing (ChIP-seq) data for Ume6-chromatin association in both wild-type and efg1{Delta}/{Delta} ndt80{Delta}/{Delta} upc2{Delta}/{Delta} backgrounds. Ume6 associates with two promoter region classes. For one class, Ume6 association is significantly decreased in the efg1{Delta}/{Delta} ndt80{Delta}/{Delta} upc2{Delta}/{Delta} background. This class includes 577 genes, many with well-established roles in biofilm formation or the related process of filamentation. For the second class, Ume6 association is unperturbed in the triple mutant background. This class includes 534 genes, some with roles in iron homeostasis (e.g., SFU1) and ergosterol synthesis (e.g., ERG11), which may contribute to the impact of Ume6 on ndt80{Delta}/{Delta} mutant azole drug sensitivity. It also includes FLO9, a putative adhesin gene that is required for the emergent biofilm state produced by efg1{Delta}/{Delta} ndt80{Delta}/{Delta} double mutants. The data show that Ume6 binding to ~half of its targets requires known partners, and Ume6 binding to ~half of its targets does not. Ume6 may bind to the latter set of promoter regions independently of any partner, or perhaps with additional partners that have yet to be discovered.
ER -