Skip to content
DraconiticResearch
Search Sources API
190,851 studies31 sourcesUpdated just now
Peer-reviewedCC BY 4.0eLife

Gcn5 and mTORC1 mediated regulation of autophagy controls Drosophila blood cell homeostasis

  • Arjun Aji Reeja1,2
  • Mincy Kunjumon1,3
  • Suraj Math1,4
  • Laxmi Kata1
  • Rohan Jayant Khadilkar1,3
4 affiliations
  1. 1Stem Cell and Tissue Homeostasis Laboratory, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India
  2. 2Champalimaud Research, Champalimaud Foundation, Lisboa, Portugal
  3. 3Homi Bhabha National Institute, Training School Complex, Mumbai, India
  4. 4Center for Genomic Medicine, Massachusetts General Hospital, Boston, United States
Read full textPDFDOICite

Abstract

Blood progenitors are regulated by a variety of systemic and nutritional cues from their environment. In the Drosophila lymph gland (LG), the Posterior Signaling Center acts as a stem cell niche striking a balance between progenitors and differentiated blood cells. Autophagy is a vital cellular process that maintains homeostasis by removing unnecessary or dysfunctional cell components through autophagic degradation and recycling. Here, using genetic perturbation analysis, we show that autophagy plays a critical role in regulating LG blood cell homeostasis. General control non-derepressible 5 (Gcn5), a histone acetyltransferase, is expressed in the primary LG lobe, and modulation of Gcn5 levels perturbs LG homeostasis. Our results show that hemocyte-specific Gcn5 modulation controls autophagic flux in the hemocytes. Furthermore, we show that modulation of mTORC1 activity can perturb hematopoiesis. Our results indicate that organismal Gcn5 levels respond to dietary shifts and are modulated by mTORC1 signaling. Chemical intervention shows that mTORC1 overrides the effect exerted by Gcn5 in regulating LG hematopoiesis. Taken together, our findings demonstrate that Gcn5 and mTORC1 regulate autophagy to maintain blood cell homeostasis in Drosophila.

Related

  1. Macrophage SELENOP controls neutrophil homeostasis through redox stress regulation in Crohn's disease
    Honggang Wang, Juan Wang, Wenliang Jiang +7
    Redox BiologyPeer-reviewedCC BYFull textGenetics & genomicsDOAJ
  2. Apelin signaling controls neuroendocrine homeostasis via Atoh8-dependent transcriptional regulation
    R. Menard, A. Neog, C. Halluin +2
    bioRxivPreprintCC BY-NC-NDFull textDevelopmental biologybioRxiv
  3. Glutaminase contributes to MYC-induced cell-autonomous autophagy and to RasV12-dependent non-autonomous autophagy in the Drosophila wing disc epithelium
    F. Destefanis, L. Conci, S. Bajaj +2
    bioRxivPreprintAll rights reservedFull textCell biologybioRxiv
  4. Neprilysin 1 in the Mushroom Body controls Feeding behavior by regulating SIFamide levels in Drosophila melanogaster
    E.-S. Baz, D. Moons, M. Pons-Vizcarra +8
    bioRxivPreprintAll rights reservedFull textZoology & animal behaviourbioRxiv
  5. Chemoreceptor TRPγ coordinates locomotor activity via regulating intracellular Ca²⁺ homeostasis in Drosophila
    Peiyuan Pang, Bomin Gao, Xiaoyi Mo +12
    Nature CommunicationsPeer-reviewedCC BY 4.0General biologyNatureCrossrefHAL

Cite

BibTeXRIS
BibTeX
@article{reeja2026gcn5,
  title = {{Gcn5 and mTORC1 mediated regulation of autophagy controls Drosophila blood cell homeostasis}},
  author = {Arjun Aji Reeja and Mincy Kunjumon and Suraj Math and Laxmi Kata and Rohan Jayant Khadilkar},
  journal = {eLife},
  year = {2026},
  month = nov,
  doi = {10.7554/elife.91615},
  url = {https://elifesciences.org/articles/91615},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Gcn5 and mTORC1 mediated regulation of autophagy controls Drosophila blood cell homeostasis
AU  - Arjun Aji Reeja
AU  - Mincy Kunjumon
AU  - Suraj Math
AU  - Laxmi Kata
AU  - Rohan Jayant Khadilkar
PY  - 2026
DA  - 2026/11/05
JO  - eLife
DO  - 10.7554/elife.91615
UR  - https://elifesciences.org/articles/91615
AB  - Blood progenitors are regulated by a variety of systemic and nutritional cues from their environment. In the Drosophila lymph gland (LG), the Posterior Signaling Center acts as a stem cell niche striking a balance between progenitors and differentiated blood cells. Autophagy is a vital cellular process that maintains homeostasis by removing unnecessary or dysfunctional cell components through autophagic degradation and recycling. Here, using genetic perturbation analysis, we show that autophagy plays a critical role in regulating LG blood cell homeostasis. General control non-derepressible 5 (Gcn5), a histone acetyltransferase, is expressed in the primary LG lobe, and modulation of Gcn5 levels perturbs LG homeostasis. Our results show that hemocyte-specific Gcn5 modulation controls autophagic flux in the hemocytes. Furthermore, we show that modulation of mTORC1 activity can perturb hematopoiesis. Our results indicate that organismal Gcn5 levels respond to dietary shifts and are modulated by mTORC1 signaling. Chemical intervention shows that mTORC1 overrides the effect exerted by Gcn5 in regulating LG hematopoiesis. Taken together, our findings demonstrate that Gcn5 and mTORC1 regulate autophagy to maintain blood cell homeostasis in Drosophila.
SN  - 2050-084X
ER  -