Skip to content
DraconiticResearch
Search Sources API
203,948 studies31 sourcesUpdated 1 min ago
Peer-reviewedCC BYPoultry science

A chicken microRNA expressing LaSota targets Newcastle disease virus phosphoprotein and impacts interferon expression.

  • Abhijeet Bakre1
  • Timothy R Satterlee2
  • Megan C Mears3
  1. 1Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratories, US National Poultry Research Center, 934 College Station Road, Athens, GA, 30605, United States. Electronic address: [email protected].
  2. 2Core Sequencing Lab, US National Poultry Research Center, 950 College Station Road, Athens, GA, 30605, United States.
  3. 3Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratories, US National Poultry Research Center, 934 College Station Road, Athens, GA, 30605, United States.
Read full textPDFDOICite

Abstract

Newcastle disease (ND), a major cause of economic loss in the poultry industry worldwide, is caused by outbreaks of virulent avian paramyxovirus type 1(APMV-1) virus. We previously demonstrated that chicken miRNA-27b-5p (gga-miR-27b-5p) can regulate the APMV-1 phosphoprotein (P) and alter viral replication. In this study, the LaSota vaccine strain of APMV-1 was used as a backbone to express the chicken pre-miRNA-27b and expected to increase intra-cellular levels of both gga-miR-27b-5p and gga-miR-27b-3p. This novel recombinant virus, rLS-miR-27b, was rescued, propagated in embryonating chicken eggs (ECEs) and did not exhibit toxicity in ECEs. Expression of both gga-miR-27b-5p and gga-miR-27b-3p was increased significantly relative to controls. An inverse correlation was observed between increased expression of gga-miR-27b-5p and reduced APMV-1 P transcripts. Additionally, increased expression of interferons (alpha, beta and lambda) and interferon stimulated gene MX1 was observed. Suppression of P did not alter the P:V ratio in rLS-27b virus infected cells. These data support the observation that miR-27b-5p expression alters viral transcriptional kinetics in cell culture without affecting overall progeny virus yield in ECEs and could be utilized to improve current APMV-1 vaccines.

Related

  1. Residue Gln370 of Newcastle disease virus nucleoprotein mediate viral inclusion body-like structure formation via interaction with residue Ser254 of viral phosphoprotein
    Wenbin Wang, Wenbin Wang, Wenbin Wang +26
    Frontiers in Cellular and Infection MicrobiologyPeer-reviewedCC BYFull textEpidemiology & public healthDOAJEurope PMC
  2. Differential Expression of Salivary microRNA Associated with Keratoconus
    Steve Mabry, Purnima Sharma, Sarah E. Nicholas +3
    Experimental eye researchPeer-reviewedLicence not statedFull textMedical imaging & radiologyPMC
  3. Cross-lineage anomaly detection of microRNA expression: a framework for deriving exploratory candidate biomarkers in sarcoma.
    Mritunjoy Dey, Piotr Remiszewski, Ewelina Perdas +3
    Frontiers in geneticsPeer-reviewedCC BYFull textOncologyEurope PMC
  4. Flunarizine changes microRNA expression in cell cultures and in a mouse model of spinal muscular atrophy
    Emeline Bon, Perrine Delers, Florent Dumont +5
    Scientific ReportsPeer-reviewedCC BY-NC-NDFull textHuman geneticsHAL
  5. Combined plasma and liquid-based cytology microRNA signatures as biomarkers of high-grade cervical lesions: association with HPV infection and p16 expression.
    Tabinda Hasan, Huda Eltayeb, Amal N Alanazi +3
    The journal of liquid biopsyPeer-reviewedCC BY-NC-NDFull textEpidemiology & public healthEurope PMC

Cite

BibTeXRIS
BibTeX
@article{bakre2026chicken,
  title = {{A chicken microRNA expressing LaSota targets Newcastle disease virus phosphoprotein and impacts interferon expression.}},
  author = {Abhijeet Bakre and Timothy R Satterlee and Megan C Mears},
  journal = {Poultry science},
  year = {2026},
  month = oct,
  doi = {10.1016/j.psj.2026.107893},
  url = {https://europepmc.org/article/MED/42863174},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - A chicken microRNA expressing LaSota targets Newcastle disease virus phosphoprotein and impacts interferon expression.
AU  - Abhijeet Bakre
AU  - Timothy R Satterlee
AU  - Megan C Mears
PY  - 2026
DA  - 2026/10/05
JO  - Poultry science
DO  - 10.1016/j.psj.2026.107893
UR  - https://europepmc.org/article/MED/42863174
AB  - Newcastle disease (ND), a major cause of economic loss in the poultry industry worldwide, is caused by outbreaks of virulent avian paramyxovirus type 1(APMV-1) virus. We previously demonstrated that chicken miRNA-27b-5p (gga-miR-27b-5p) can regulate the APMV-1 phosphoprotein (P) and alter viral replication. In this study, the LaSota vaccine strain of APMV-1 was used as a backbone to express the chicken pre-miRNA-27b and expected to increase intra-cellular levels of both gga-miR-27b-5p and gga-miR-27b-3p. This novel recombinant virus, rLS-miR-27b, was rescued, propagated in embryonating chicken eggs (ECEs) and did not exhibit toxicity in ECEs. Expression of both gga-miR-27b-5p and gga-miR-27b-3p was increased significantly relative to controls. An inverse correlation was observed between increased expression of gga-miR-27b-5p and reduced APMV-1 P transcripts. Additionally, increased expression of interferons (alpha, beta and lambda) and interferon stimulated gene MX1 was observed. Suppression of P did not alter the P:V ratio in rLS-27b virus infected cells. These data support the observation that miR-27b-5p expression alters viral transcriptional kinetics in cell culture without affecting overall progeny virus yield in ECEs and could be utilized to improve current APMV-1 vaccines.
SN  - 0032-5791
SN  - 1525-3171
ER  -