A myosin filament cage drives multiple aspects of mitosis unique to cells dividing within a polarized epithelium
- 1Cell and Developmental Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD
Abstract
Here we used mouse intestinal organoids expressing endogenously tagged myosin to clarify its roles in those aspects of mitosis unique to division within a polarized epithelium. Super-resolution imaging of rounded metaphase cells revealed a never-before-seen myosin filament cage that only forms in this context, exhibits apical/basal polarity, maintains roundness by resisting surrounding tissue pressure, and repositions laterally to form a contractile ring predetermined to drive planar division and asymmetric cleavage furrow progression. Attenuating cage function inhibited interkinetic nuclear migration and metaphase cell rounding, causing the division plane to shift from planar to orthogonal and resulting in organoids possessing multiple lumens. Following the apical domain marker ZO-1 showed that the new lumens originate at the interface between stacked daughter cells created by orthogonal divisions. We conclude that cells dividing within polarized epithelia create a myosin filament cage designed specifically to drive those aspects of cell division unique to their context.
Related
- Effect of Varying Cigarette Smoke Concentrations and Durations on the Retinal Pigment Epithelium
- Glutaminase contributes to MYC-induced cell-autonomous autophagy and to RasV12-dependent non-autonomous autophagy in the Drosophila wing disc epithelium
- NOSE-OE: A molecular signaling map of the olfactory epithelium
- DNA Methylation Patterns Between Serous Uterine and Ovarian Carcinomas Indicate Shared Markers with Fallopian Tube Epithelium and Distinctions Consistent with Histopathology
- CDHR3 Asthma-Risk Genotype Affects Susceptibility of Airway Epithelium to Rhinovirus C Infections
BibTeX
@misc{yousafzai2026myosin,
title = {{A myosin filament cage drives multiple aspects of mitosis unique to cells dividing within a polarized epithelium}},
author = {S. Yousafzai and K. Remmert and A. Gasilina and G. Perry and L. Boley and C. Alexander and A. Pedrosa and D. Schrock and Y.-I. Yim and X. Wu and J. A. Hammer},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.25.761724},
url = {https://www.biorxiv.org/content/10.64898/2026.10.25.761724v1},
note = {CC0},
}
RIS
TY - UNPB
TI - A myosin filament cage drives multiple aspects of mitosis unique to cells dividing within a polarized epithelium
AU - S. Yousafzai
AU - K. Remmert
AU - A. Gasilina
AU - G. Perry
AU - L. Boley
AU - C. Alexander
AU - A. Pedrosa
AU - D. Schrock
AU - Y.-I. Yim
AU - X. Wu
AU - J. A. Hammer
PY - 2026
DA - 2026/10/25
PB - bioRxiv
DO - 10.64898/2026.10.25.761724
UR - https://www.biorxiv.org/content/10.64898/2026.10.25.761724v1
AB - Here we used mouse intestinal organoids expressing endogenously tagged myosin to clarify its roles in those aspects of mitosis unique to division within a polarized epithelium. Super-resolution imaging of rounded metaphase cells revealed a never-before-seen myosin filament cage that only forms in this context, exhibits apical/basal polarity, maintains roundness by resisting surrounding tissue pressure, and repositions laterally to form a contractile ring predetermined to drive planar division and asymmetric cleavage furrow progression. Attenuating cage function inhibited interkinetic nuclear migration and metaphase cell rounding, causing the division plane to shift from planar to orthogonal and resulting in organoids possessing multiple lumens. Following the apical domain marker ZO-1 showed that the new lumens originate at the interface between stacked daughter cells created by orthogonal divisions. We conclude that cells dividing within polarized epithelia create a myosin filament cage designed specifically to drive those aspects of cell division unique to their context.
ER -