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Peer-reviewedCC BY 4.0Frontiers in Oncology

FLT3 expression and immune-associated transcriptional signatures in prostate cancer

  • Yongqiang Cui1
  • Tianmou Song1
  • Xingyue Wang1
  • Zijin Wan1
  • Lijun Mao1
  1. 1Department of Urology, The Affiliated Hospital of Xuzhou Medical University
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Abstract

Background: Bulk transcriptomic profiles combine signals from malignant, stromal, and immune cells. This study examined immune-associated gene expression in prostate cancer using bulk and single-cell transcriptomic data, and separately assessed the effects of FLT3 knockdown in prostate cancer cell lines.

Methods: We screened 502 sample records from 497 patients and identified genes shared by ImmuneScore-associated differential expression and a weighted gene co-expression network module. Candidate genes were compared with four functional gene sets. We analyzed associations between FLT3 expression and immune-related signatures, examined single-cell expression patterns, and assessed FLT3 knockdown using two independent siRNAs in DU145 and 22Rv1 cells.

Results: The screening identified 64 shared genes, and comparison with the functional gene sets prioritized FLT3 and AIM2. FLT3 expression was associated with several immune-cell signatures; associations were stronger for total T-cell and B-cell signatures than for the CD8-positive T-cell signature. Single-cell analyses suggested FLT3 expression in cells annotated as monocytes. In DU145 and 22Rv1 cells, both FLT3-targeting siRNAs reduced growth and colony formation, reduced Transwell migration and invasion readouts, and altered epithelial–mesenchymal transition markers.

Conclusion: The tissue analyses support an association between FLT3 expression and immune-related transcriptional signals. The cell-line findings describe a separate in vitro phenotype and do not establish how FLT3 relates to lymphocyte infiltration. These findings do not establish a causal immune-regulatory mechanism or prognostic utility.

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Cite

BibTeXRIS
BibTeX
@article{cui2026flt3,
  title = {{FLT3 expression and immune-associated transcriptional signatures in prostate cancer}},
  author = {Yongqiang Cui and Tianmou Song and Xingyue Wang and Zijin Wan and Lijun Mao},
  journal = {Frontiers in Oncology},
  year = {2026},
  month = oct,
  doi = {10.3389/fonc.2026.1970520},
  url = {https://www.frontiersin.org/articles/10.3389/fonc.2026.1970520/full},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - FLT3 expression and immune-associated transcriptional signatures in prostate cancer
AU  - Yongqiang Cui
AU  - Tianmou Song
AU  - Xingyue Wang
AU  - Zijin Wan
AU  - Lijun Mao
PY  - 2026
DA  - 2026/10/23
JO  - Frontiers in Oncology
DO  - 10.3389/fonc.2026.1970520
UR  - https://www.frontiersin.org/articles/10.3389/fonc.2026.1970520/full
AB  - Background: Bulk transcriptomic profiles combine signals from malignant, stromal, and immune cells. This study examined immune-associated gene expression in prostate cancer using bulk and single-cell transcriptomic data, and separately assessed the effects of FLT3 knockdown in prostate cancer cell lines.  Methods: We screened 502 sample records from 497 patients and identified genes shared by ImmuneScore-associated differential expression and a weighted gene co-expression network module. Candidate genes were compared with four functional gene sets. We analyzed associations between FLT3 expression and immune-related signatures, examined single-cell expression patterns, and assessed FLT3 knockdown using two independent siRNAs in DU145 and 22Rv1 cells.  Results: The screening identified 64 shared genes, and comparison with the functional gene sets prioritized FLT3 and AIM2. FLT3 expression was associated with several immune-cell signatures; associations were stronger for total T-cell and B-cell signatures than for the CD8-positive T-cell signature. Single-cell analyses suggested FLT3 expression in cells annotated as monocytes. In DU145 and 22Rv1 cells, both FLT3-targeting siRNAs reduced growth and colony formation, reduced Transwell migration and invasion readouts, and altered epithelial–mesenchymal transition markers.  Conclusion: The tissue analyses support an association between FLT3 expression and immune-related transcriptional signals. The cell-line findings describe a separate in vitro phenotype and do not establish how FLT3 relates to lymphocyte infiltration. These findings do not establish a causal immune-regulatory mechanism or prognostic utility.
SN  - 2234-943X
ER  -