Alkaloidal constituents of Crinum asiaticum L., toxicology and in silico studies against some piercing-sucking pests
Abstract
Many important crops around the world have been severely damaged by piercing-sucking pests. These pests can be managed with botanical pesticides. A major alkaloid, hippeastrine- N -oxide 3 , was isolated from the Crinum asiaticum alkaloid fraction. In addition, four known compounds, 11-hydroxyvittatine- N -oxide 1 , lycoramine- N -oxide 2 , lycorine 4 , and vittatine 5 , were identified by LC-MS/MS. ¹ H NMR and ESI-MS spectroscopy were used to identify the structure of the isolated compound. Pesticidal activity of the alkaloid fraction and hippeastrine N -oxide was tested against four piercing-sucking pests along with azadirachtin as a positive control under laboratory conditions. Hippeastrine- N -oxide showed high toxicity against four pests, especially Aphis craccivora , compared to the control, with LC ₅₀ values of 16.00 and 26.90 mg/L, respectively. The LC ₅₀ value of hippeastrine- N -oxide was used to evaluate the biochemical characteristics of six Aphis craccivora enzymes. Molecular docking and fingerprint analysis of the lead compound revealed strong binding interactions with key amino acid residues in the target enzymes, further validating the experimental findings. These computational results make it possible to understand the mechanism of action observed in the laboratory assays. These findings indicate that the C. asiaticum alkaloid fraction, and hippeastrine N ‑oxide in particular, exhibit insecticidal activity against the piercing‑sucking pests tested under laboratory conditions, supporting further evaluation of their potential as botanical pest-control agents.
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BibTeX
@article{elrokh2026alkaloidal,
title = {{Alkaloidal constituents of Crinum asiaticum L., toxicology and in silico studies against some piercing-sucking pests}},
author = {Ahmed Ramadan El‑Rokh and Ahmed E. Suliman and Ahmed R. Elaraby and Tamer M. Ibrahim and Ahmed BahaaEldin Darwish and Samira A. Abd El-Salam},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-71529-8},
url = {https://www.nature.com/articles/s41598-026-71529-8},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Alkaloidal constituents of Crinum asiaticum L., toxicology and in silico studies against some piercing-sucking pests
AU - Ahmed Ramadan El‑Rokh
AU - Ahmed E. Suliman
AU - Ahmed R. Elaraby
AU - Tamer M. Ibrahim
AU - Ahmed BahaaEldin Darwish
AU - Samira A. Abd El-Salam
PY - 2026
DA - 2026/10/05
JO - Scientific Reports
DO - 10.1038/s41598-026-71529-8
UR - https://www.nature.com/articles/s41598-026-71529-8
AB - Many important crops around the world have been severely damaged by piercing-sucking pests. These pests can be managed with botanical pesticides. A major alkaloid, hippeastrine- N -oxide 3 , was isolated from the Crinum asiaticum alkaloid fraction. In addition, four known compounds, 11-hydroxyvittatine- N -oxide 1 , lycoramine- N -oxide 2 , lycorine 4 , and vittatine 5 , were identified by LC-MS/MS. ¹ H NMR and ESI-MS spectroscopy were used to identify the structure of the isolated compound. Pesticidal activity of the alkaloid fraction and hippeastrine N -oxide was tested against four piercing-sucking pests along with azadirachtin as a positive control under laboratory conditions. Hippeastrine- N -oxide showed high toxicity against four pests, especially Aphis craccivora , compared to the control, with LC ₅₀ values of 16.00 and 26.90 mg/L, respectively. The LC ₅₀ value of hippeastrine- N -oxide was used to evaluate the biochemical characteristics of six Aphis craccivora enzymes. Molecular docking and fingerprint analysis of the lead compound revealed strong binding interactions with key amino acid residues in the target enzymes, further validating the experimental findings. These computational results make it possible to understand the mechanism of action observed in the laboratory assays. These findings indicate that the C. asiaticum alkaloid fraction, and hippeastrine N ‑oxide in particular, exhibit insecticidal activity against the piercing‑sucking pests tested under laboratory conditions, supporting further evaluation of their potential as botanical pest-control agents.
SN - 2045-2322
ER -