Skin-Whitening and Anti-Photoaging Activities of Limosilactobacillus reuteri DS0333 Culture Supernatant Produced in Unripe Rubus occidentalis L. Extract-Supplemented Medium.
- 1Department of Biocosmetics, Dongshin University, 185, Gunjae-ro, Naju 58245, Jeonnam, Republic of Korea.
- 2RAPPOL, 30, Okcheon-gil, Suncheon 57941, Jeonnam, Republic of Korea.
- 3Department of Petrochemical Process, Hanyeong University, 18-43, Janggunsan-gil, Yeosu 59720, Jeonnam, Republic of Korea.
Abstract
This study investigated the skin-whitening and anti-photoaging activities of a culture supernatant obtained by cultivating Limosilactobacillus reuteri DS0333 in chemically defined medium (CDM) supplemented with unripe Rubus occidentalis L. extract (UROE). DS0333 was cultured in UROE-supplemented medium, and the resulting DS0333/(CDM+UROE) group was evaluated alongside all control and comparison groups. The DS0333/(CDM+UROE) culture supernatant showed enhanced DPPH and ABTS radical scavenging activities compared with all other experimental groups. In α-MSH-stimulated B16F10 cells, the culture supernatant reduced melanin content and intracellular tyrosinase activity and downregulated MITF, TRP-1, and TRP-2 expression. In UVB-irradiated human dermal fibroblasts, the culture supernatant increased type I procollagen production, reduced MMP-1 levels, and suppressed MMP-1/MMP-3 expression through attenuation of the MAPK/AP-1 signaling pathway. These results suggest that culturing DS0333 in UROE-supplemented medium enhances skin-whitening and anti-photoaging activities, potentially through cultivation-associated modification of extracellular metabolites in the culture matrix. Therefore, the DS0333/(CDM+UROE) culture supernatant may serve as a promising multifunctional cosmetic ingredient for skin applications.
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BibTeX
@article{song2026skinwhitening,
title = {{Skin-Whitening and Anti-Photoaging Activities of Limosilactobacillus reuteri DS0333 Culture Supernatant Produced in Unripe Rubus occidentalis L. Extract-Supplemented Medium.}},
author = {Nu Ri Song and Seo Yeon Shin and Jae Hee Byun and Seon Ju Kim and Hye Young Kim and Won Seok Kim and Kyung Mok Park},
journal = {Journal of microbiology and biotechnology},
year = {2026},
month = oct,
doi = {10.4014/jmb.2606.06047},
url = {https://europepmc.org/article/MED/42897972},
note = {CC BY},
}
RIS
TY - JOUR
TI - Skin-Whitening and Anti-Photoaging Activities of Limosilactobacillus reuteri DS0333 Culture Supernatant Produced in Unripe Rubus occidentalis L. Extract-Supplemented Medium.
AU - Nu Ri Song
AU - Seo Yeon Shin
AU - Jae Hee Byun
AU - Seon Ju Kim
AU - Hye Young Kim
AU - Won Seok Kim
AU - Kyung Mok Park
PY - 2026
DA - 2026/10/08
JO - Journal of microbiology and biotechnology
DO - 10.4014/jmb.2606.06047
UR - https://europepmc.org/article/MED/42897972
AB - This study investigated the skin-whitening and anti-photoaging activities of a culture supernatant obtained by cultivating Limosilactobacillus reuteri DS0333 in chemically defined medium (CDM) supplemented with unripe Rubus occidentalis L. extract (UROE). DS0333 was cultured in UROE-supplemented medium, and the resulting DS0333/(CDM+UROE) group was evaluated alongside all control and comparison groups. The DS0333/(CDM+UROE) culture supernatant showed enhanced DPPH and ABTS radical scavenging activities compared with all other experimental groups. In α-MSH-stimulated B16F10 cells, the culture supernatant reduced melanin content and intracellular tyrosinase activity and downregulated MITF, TRP-1, and TRP-2 expression. In UVB-irradiated human dermal fibroblasts, the culture supernatant increased type I procollagen production, reduced MMP-1 levels, and suppressed MMP-1/MMP-3 expression through attenuation of the MAPK/AP-1 signaling pathway. These results suggest that culturing DS0333 in UROE-supplemented medium enhances skin-whitening and anti-photoaging activities, potentially through cultivation-associated modification of extracellular metabolites in the culture matrix. Therefore, the DS0333/(CDM+UROE) culture supernatant may serve as a promising multifunctional cosmetic ingredient for skin applications.
SN - 1017-7825
SN - 1738-8872
ER -