Genome-Wide Marker-Trait Analysis of Agronomic and Biomass-Related Traits in an Interspecific Miscanthus F₁ Population Grown on Reclaimed Mine Land.
6 affiliations
- 1College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
- 2Shandong Key Laboratory of Peanut Breeding, Shandong Peanut Research Institute, Qingdao 266100, China.
- 3Botanical Garden, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China.
- 4China National Botanical Garden, Beijing 100093, China.
- 5College of Biological Engineering, Binzhou Polytechnic University, Binzhou 256603, China.
- 6College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Abstract
Miscanthus is a perennial bioenergy crop suited to marginal and degraded land, yet relatively little is known about the genetic basis of biomass-related variation in interspecific hybrids across years. We evaluated 100 F₁ genotypes from Miscanthus sacchariflorus × M. lutarioriparius for two growing seasons at a reclaimed mine site and analyzed 16 agronomic, biomass-related, and physiological traits using an existing linkage map of 461 SNP marker positions across 19 linkage groups. No single-trait peak exceeded its trait-specific 5% permutation threshold. The clearest repeated suggestive signal occurred in the proximal region of LG9: marker2342 (11.571 cM) showed the strongest across-year signals for plant height and tiller length, while the adjacent marker2343 (10.561 cM) gave the strongest across-year signal for tiller number. Four correlated architecture traits were also summarized by principal component analysis. PC1 explained 73.2-76.6% of their variation, and in 2019 marker0580 on LG3 (12.516 cM) exceeded the genome-wide threshold (LOD 3.67 vs. 3.47). This association remained significant after removal of the single rare genotype-class observation but was not reproduced in 2018; formal marker × year interaction tests confirmed temporal heterogeneity for all four component traits. Biomass and physiological signals were weaker and less repeatable. Thus, the study identifies a repeated but moderate LG9 pattern and a stronger, temporally variable LG3 signal expressed at this reclaimed site; it does not establish loci specifically conferring adaptation to reclaimed mine land. These regions provide priorities for validation using larger populations, denser marker coverage, additional years, and contrasting environments before their value for marker-assisted breeding can be assessed.
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BibTeX
@article{xiao2026genomewide,
title = {{Genome-Wide Marker-Trait Analysis of Agronomic and Biomass-Related Traits in an Interspecific Miscanthus F₁ Population Grown on Reclaimed Mine Land.}},
author = {Liang Xiao and Xuhong Zhao and Cong Lin and Hui Feng and Zili Yi and Hongmei Huang and Mingxi Liu},
journal = {Plants (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/plants15203172},
url = {https://europepmc.org/article/MED/42943124},
note = {CC BY},
}
RIS
TY - JOUR
TI - Genome-Wide Marker-Trait Analysis of Agronomic and Biomass-Related Traits in an Interspecific Miscanthus F₁ Population Grown on Reclaimed Mine Land.
AU - Liang Xiao
AU - Xuhong Zhao
AU - Cong Lin
AU - Hui Feng
AU - Zili Yi
AU - Hongmei Huang
AU - Mingxi Liu
PY - 2026
DA - 2026/10/16
JO - Plants (Basel, Switzerland)
DO - 10.3390/plants15203172
UR - https://europepmc.org/article/MED/42943124
AB - Miscanthus is a perennial bioenergy crop suited to marginal and degraded land, yet relatively little is known about the genetic basis of biomass-related variation in interspecific hybrids across years. We evaluated 100 F₁ genotypes from Miscanthus sacchariflorus × M. lutarioriparius for two growing seasons at a reclaimed mine site and analyzed 16 agronomic, biomass-related, and physiological traits using an existing linkage map of 461 SNP marker positions across 19 linkage groups. No single-trait peak exceeded its trait-specific 5% permutation threshold. The clearest repeated suggestive signal occurred in the proximal region of LG9: marker2342 (11.571 cM) showed the strongest across-year signals for plant height and tiller length, while the adjacent marker2343 (10.561 cM) gave the strongest across-year signal for tiller number. Four correlated architecture traits were also summarized by principal component analysis. PC1 explained 73.2-76.6% of their variation, and in 2019 marker0580 on LG3 (12.516 cM) exceeded the genome-wide threshold (LOD 3.67 vs. 3.47). This association remained significant after removal of the single rare genotype-class observation but was not reproduced in 2018; formal marker × year interaction tests confirmed temporal heterogeneity for all four component traits. Biomass and physiological signals were weaker and less repeatable. Thus, the study identifies a repeated but moderate LG9 pattern and a stronger, temporally variable LG3 signal expressed at this reclaimed site; it does not establish loci specifically conferring adaptation to reclaimed mine land. These regions provide priorities for validation using larger populations, denser marker coverage, additional years, and contrasting environments before their value for marker-assisted breeding can be assessed.
SN - 2223-7747
ER -