Genomic insight into Enterococcus faecium biofilm formation isolated from catheter associated urinary tract infection
Abstract
Background and Objectives: Enterococcus faecium is a major nosocomial pathogen frequently associated with catheter-re- lated infections and biofilm-mediated antimicrobial resistance. This study aimed to investigate the genomic determinants and structural characteristics underlying biofilm formation in a clinical E. faecium isolate recovered from a catheter-associated urinary tract infection (CAUTI). Materials and Methods: A clinical isolate, E. faecium MFKCC-BF.UR33, was subjected to whole-genome sequencing and comprehensive genome annotation to identify biofilm-associated and antimicrobial resistance genes. Phylogenetic analysis was performed to determine its relatedness to reference clinical strains. Structural modeling of selected biofilm-associated proteins was conducted using AlphaFold2 to assess their three-dimensional conformations and potential as therapeutic targets. Results: Whole-genome sequencing revealed a 3.32 Mb genome with a G+C content of 37.35%, comprising 2,321 predicted coding sequences and no detectable plasmids. Genome annotation identified several biofilm-associated genes, including sgrA, acm, fss3, scm, and efbA, as well as antimicrobial resistance genes (antA/B, tetM, tetL, efmA, and liaFSR). Phylogeneticanalysis placed the isolate within the E. faecium clade, closely related to other clinical reference strains. Structural modeling demonstrated high-confidence 3D structures for Fss3 and EfbA proteins. Conclusion: The integration of genomic and structural analyses provides insights into the genetic and structural basis of biofilm formation in E. faecium and establishes a foundation for future functional and structure-based investigations.
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BibTeX
@article{rahayu2026genomic,
title = {{Genomic insight into Enterococcus faecium biofilm formation isolated from catheter associated urinary tract infection}},
author = {Ayu Rahayu and Sayyidah Auliany Aminy and Dian Asti Umaroh and Niar Gusnaniar and Akhirta Atikana and Puspita Lisdiyanti and Estelle Perraud and Christine Imbert and Titik Nuryastuti},
journal = {Iranian Journal of Microbiology},
year = {2026},
month = oct,
doi = {10.18502/ijm.v18i5.22866},
url = {https://hal.science/hal-05788268v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Genomic insight into Enterococcus faecium biofilm formation isolated from catheter associated urinary tract infection
AU - Ayu Rahayu
AU - Sayyidah Auliany Aminy
AU - Dian Asti Umaroh
AU - Niar Gusnaniar
AU - Akhirta Atikana
AU - Puspita Lisdiyanti
AU - Estelle Perraud
AU - Christine Imbert
AU - Titik Nuryastuti
PY - 2026
DA - 2026/10/09
JO - Iranian Journal of Microbiology
DO - 10.18502/ijm.v18i5.22866
UR - https://hal.science/hal-05788268v1
AB - Background and Objectives: Enterococcus faecium is a major nosocomial pathogen frequently associated with catheter-re- lated infections and biofilm-mediated antimicrobial resistance. This study aimed to investigate the genomic determinants and structural characteristics underlying biofilm formation in a clinical E. faecium isolate recovered from a catheter-associated urinary tract infection (CAUTI). Materials and Methods: A clinical isolate, E. faecium MFKCC-BF.UR33, was subjected to whole-genome sequencing and comprehensive genome annotation to identify biofilm-associated and antimicrobial resistance genes. Phylogenetic analysis was performed to determine its relatedness to reference clinical strains. Structural modeling of selected biofilm-associated proteins was conducted using AlphaFold2 to assess their three-dimensional conformations and potential as therapeutic targets. Results: Whole-genome sequencing revealed a 3.32 Mb genome with a G+C content of 37.35%, comprising 2,321 predicted coding sequences and no detectable plasmids. Genome annotation identified several biofilm-associated genes, including sgrA, acm, fss3, scm, and efbA, as well as antimicrobial resistance genes (antA/B, tetM, tetL, efmA, and liaFSR). Phylogeneticanalysis placed the isolate within the E. faecium clade, closely related to other clinical reference strains. Structural modeling demonstrated high-confidence 3D structures for Fss3 and EfbA proteins. Conclusion: The integration of genomic and structural analyses provides insights into the genetic and structural basis of biofilm formation in E. faecium and establishes a foundation for future functional and structure-based investigations.
SN - 2008-3289
SN - 2008-4447
ER -