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Peer-reviewedCC BYmicroPublication Biology

miR-10-3p, but not miR-1006-3p, regulates mei-P26 expression in the Drosophila testis

  • Shallinie Thangadurai1
  • Elena Pak1
  • Isabella Hammock2
  • Alexander Murashov1
  1. 1Comparative Biomedical Sciences, Louisiana State University, Baton Rouge, LA, United States
  2. 2Saint Joseph's Academy
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Abstract

MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression, yet predicted miRNA target interactions often require experimental validation. TargetScan Fly predicts that both miR-10-3p and miR-1006-3p target the Drosophila RNA-binding protein Mei-P26, a critical regulator of germline development and miRNA biogenesis. Here, we experimentally demonstrate that overexpression of miR-10-3p is sufficient to significantly suppress mei-P26 mRNA level in the testis. Consistently, inhibition of miR-10 using sponge increased mei-P26 positive spermatocytes in testis. Together, these findings suggest that miR-10-3p, but not miR-1006-3p, functionally regulates mei-P26 in Drosophila testis, highlighting selective engagement of canonical miRNA pathways in mei-P26 regulation.

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Cite

BibTeXRIS
BibTeX
@article{thangadurai2026mir103p,
  title = {{miR-10-3p, but not miR-1006-3p, regulates mei-P26 expression in the Drosophila testis}},
  author = {Shallinie Thangadurai and Elena Pak and Isabella Hammock and Alexander Murashov},
  journal = {microPublication Biology},
  year = {2026},
  month = aug,
  doi = {10.17912/micropub.biology.002187},
  url = {https://doi.org/10.17912/micropub.biology.002187},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - miR-10-3p, but not miR-1006-3p, regulates mei-P26 expression in the Drosophila testis
AU  - Shallinie Thangadurai
AU  - Elena Pak
AU  - Isabella Hammock
AU  - Alexander Murashov
PY  - 2026
DA  - 2026/08/01
JO  - microPublication Biology
DO  - 10.17912/micropub.biology.002187
UR  - https://doi.org/10.17912/micropub.biology.002187
AB  - MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression, yet predicted miRNA target interactions often require experimental validation. TargetScan Fly predicts that both miR-10-3p and miR-1006-3p target the Drosophila RNA-binding protein Mei-P26, a critical regulator of germline development and miRNA biogenesis. Here, we experimentally demonstrate that overexpression of miR-10-3p is sufficient to significantly suppress mei-P26 mRNA level in the testis. Consistently, inhibition of miR-10 using sponge increased mei-P26 positive spermatocytes in testis. Together, these findings suggest that miR-10-3p, but not miR-1006-3p, functionally regulates mei-P26 in Drosophila testis, highlighting selective engagement of canonical miRNA pathways in mei-P26 regulation.
SN  - 2578-9430
ER  -