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Peer-reviewedCC BYRSC chemical biology

ARCaDia-selected short DNA core positions a latent warhead for protein-gated covalent capture.

  • Chaohui Li1
  • Naoyuki Miyashita1,2
  • Masayasu Kuwahara3
  • Jay Yang1,4
  • Masumi Taki1,5
5 affiliations
  1. 1Department of Engineering Science, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC), 1-5-1 Chofugaoka, Chofu Tokyo 182-8585 Japan [email protected] [email protected] +81-42-443-5980.
  2. 2Department of Biological Systems Engineering, Graduate School of Biology-Oriented Science and Technology, KINDAI University, 930 Nishimitani, Kinokawa Wakayama 649-6493 Japan.
  3. 3Graduate School of Integrated Basic Sciences, Nihon University, Setagaya-ku Tokyo 156-8550 Japan.
  4. 4School of Medicine and Public Health, University of Wisconsin Madison Wisconsin 53706 USA.
  5. 5Institute for Advanced Science, UEC Chofu 182-8585 Japan.
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Abstract

A single-round affinity/reactivity co-selection of aptamer (ARCaDia), combined with Fast String-Based Clustering (FSBC) analysis of the selection output, identified a compact 10-nucleotide DNA-warhead core motif for streptavidin-selective covalent capture, showing that protein-gated covalent chemistry can arise from a short sequence-dependent placement of a latent warhead in a biotin-sensitive reaction microenvironment. This demonstrates that functional warhead-positioning information is already present after a single selection round.

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Cite

BibTeXRIS
BibTeX
@article{li2026arcadiaselected,
  title = {{ARCaDia-selected short DNA core positions a latent warhead for protein-gated covalent capture.}},
  author = {Chaohui Li and Naoyuki Miyashita and Masayasu Kuwahara and Jay Yang and Masumi Taki},
  journal = {RSC chemical biology},
  year = {2026},
  month = oct,
  doi = {10.1039/d6cb00303f},
  url = {https://europepmc.org/article/MED/42891161},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - ARCaDia-selected short DNA core positions a latent warhead for protein-gated covalent capture.
AU  - Chaohui Li
AU  - Naoyuki Miyashita
AU  - Masayasu Kuwahara
AU  - Jay Yang
AU  - Masumi Taki
PY  - 2026
DA  - 2026/10/16
JO  - RSC chemical biology
DO  - 10.1039/d6cb00303f
UR  - https://europepmc.org/article/MED/42891161
AB  - A single-round affinity/reactivity co-selection of aptamer (ARCaDia), combined with Fast String-Based Clustering (FSBC) analysis of the selection output, identified a compact 10-nucleotide DNA-warhead core motif for streptavidin-selective covalent capture, showing that protein-gated covalent chemistry can arise from a short sequence-dependent placement of a latent warhead in a biotin-sensitive reaction microenvironment. This demonstrates that functional warhead-positioning information is already present after a single selection round.
SN  - 2633-0679
ER  -