p14 Arf Promotes Small Ubiquitin-like Modifier Conjugation of Werners Helicase
Abstract
Here we demonstrate a novel p53-independent interaction between the nucleolar tumor suppressors, p14 Arf and Werners helicase (WRN). Binding of p14 Arf to WRN is multivalent and resembles the binding of p14 Arf to Mdm2. Residues 2-14 and 82-101 of p14 Arf and residues in the central region and C terminus of WRN have particular importance for binding. p14 Arf promotes small ubiquitin-like modifier (SUMO) modification of WRN in a synergistic manner with the SUMO-conjugating enzyme, UBCH9. p14 Arf causes redistribution of WRN within the nucleus, and this effect is reversed by expression of a SUMO-specific protease, thus implicating the SUMO conjugation pathway in WRN re-localization. We establish that the ability to promote SUMO conjugation is a general property of the p14 Arf tumor suppressor.
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BibTeX
@article{woods2026promotes,
title = {{p14 Arf Promotes Small Ubiquitin-like Modifier Conjugation of Werners Helicase}},
author = {Yvonne Woods and Dimitris Xirodimas and Alan Prescott and Alison Sparks and David Lane and Mark Saville},
journal = {Journal of Biological Chemistry},
year = {2026},
month = sep,
doi = {10.1074/jbc.m405414200},
url = {https://hal.science/hal-05751940v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - p14 Arf Promotes Small Ubiquitin-like Modifier Conjugation of Werners Helicase
AU - Yvonne Woods
AU - Dimitris Xirodimas
AU - Alan Prescott
AU - Alison Sparks
AU - David Lane
AU - Mark Saville
PY - 2026
DA - 2026/09/16
JO - Journal of Biological Chemistry
DO - 10.1074/jbc.m405414200
UR - https://hal.science/hal-05751940v1
AB - Here we demonstrate a novel p53-independent interaction between the nucleolar tumor suppressors, p14 Arf and Werners helicase (WRN). Binding of p14 Arf to WRN is multivalent and resembles the binding of p14 Arf to Mdm2. Residues 2-14 and 82-101 of p14 Arf and residues in the central region and C terminus of WRN have particular importance for binding. p14 Arf promotes small ubiquitin-like modifier (SUMO) modification of WRN in a synergistic manner with the SUMO-conjugating enzyme, UBCH9. p14 Arf causes redistribution of WRN within the nucleus, and this effect is reversed by expression of a SUMO-specific protease, thus implicating the SUMO conjugation pathway in WRN re-localization. We establish that the ability to promote SUMO conjugation is a general property of the p14 Arf tumor suppressor.
SN - 0021-9258
SN - 1083-351X
ER -