Distinct regulation across the transcription cycle drives mitotic transcriptional silencing
- 1Universidade Católica Portuguesa
Abstract
In most organisms, as cells enter mitosis, transcription is globally silenced. While individual studies have shown that each stage of the transcription cycle can be modulated during mitosis, how initiation, elongation, and termination are temporally and kinetically regulated to ensure transcriptional silencing remains unclear. Here, we combine real-time quantitative imaging of ongoing transcription in early Drosophila embryos with mathematical modelling to systematically dissect how transcriptional kinetics change as cells progress into mitosis. We found that transcription initiation undergoes a gradual repression starting in prophase, while elongation rates remain unaltered. After nuclear envelope breakdown, an abrupt mechanism causes premature termination of elongating RNA, driven at least in part by the SNF-helicase-like protein Transcription Termination factor 2 / Lodestar (TTF2/Lds). Our study provides a quantitative framework for understanding how transcription is modulated across the cell cycle, demonstrating that mitotic repression arises from multiple processes with different dynamics that collectively ensure the precise shutdown of gene expression.
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BibTeX
@misc{gaetani2026distinct,
title = {{Distinct regulation across the transcription cycle drives mitotic transcriptional silencing}},
author = {P. Gaetani and A. Tavares and T. Paixao and R. A. Oliveira},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.12.758451},
url = {https://www.biorxiv.org/content/10.64898/2026.10.12.758451v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Distinct regulation across the transcription cycle drives mitotic transcriptional silencing
AU - P. Gaetani
AU - A. Tavares
AU - T. Paixao
AU - R. A. Oliveira
PY - 2026
DA - 2026/10/12
PB - bioRxiv
DO - 10.64898/2026.10.12.758451
UR - https://www.biorxiv.org/content/10.64898/2026.10.12.758451v1
AB - In most organisms, as cells enter mitosis, transcription is globally silenced. While individual studies have shown that each stage of the transcription cycle can be modulated during mitosis, how initiation, elongation, and termination are temporally and kinetically regulated to ensure transcriptional silencing remains unclear. Here, we combine real-time quantitative imaging of ongoing transcription in early Drosophila embryos with mathematical modelling to systematically dissect how transcriptional kinetics change as cells progress into mitosis. We found that transcription initiation undergoes a gradual repression starting in prophase, while elongation rates remain unaltered. After nuclear envelope breakdown, an abrupt mechanism causes premature termination of elongating RNA, driven at least in part by the SNF-helicase-like protein Transcription Termination factor 2 / Lodestar (TTF2/Lds). Our study provides a quantitative framework for understanding how transcription is modulated across the cell cycle, demonstrating that mitotic repression arises from multiple processes with different dynamics that collectively ensure the precise shutdown of gene expression.
ER -