Full-length 16S profiling reveals individualized gut microbiota dynamics during short-duration spaceflight
- 1Szegedi Tudomanyegyetem Altalanos Orvostudomanyi Kar
Abstract
Human spaceflight may perturb the gut microbiota, but densely sampled short missions remain poorly characterized. We profiled 27 phase-matched fecal samples from two astronauts during an 18-day International Space Station mission and one ground-based participant following the same daily schedule using Oxford Nanopore full-length 16S sequencing. Participant identity dominated genus-level Bray-Curtis variation (R2 = 0.489, p < 0.001). In astronaut-only community analyses, mission phase explained 23.4% of genus-level (p = 0.035) and 22.3% of species-level (p = 0.021) variation. Astronauts showed greater displacement from personal baselines than B1 (0.331 versus 0.171) and 1.58-fold higher volatility. Astronaut-only taxon models identified 2 of 81 genera and 5 of 139 species; Collinsella increased from quarantine to orbit (coefficient = 2.586, q = 0.037). Thus, the short-duration spaceflight interval was accompanied by individualized, temporally localized community and taxon shifts rather than uniform microbiota restructuring.
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BibTeX
@misc{gulyas2026fulllength,
title = {{Full-length 16S profiling reveals individualized gut microbiota dynamics during short-duration spaceflight}},
author = {G. Gulyas and B. Kakuk and A. Dormo and I. Prazsak and T. Jaray and Z. Csabai and A. T. Schlegl and Z. Boldogkoi and D. Tombacz},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.01.748461},
url = {https://www.biorxiv.org/content/10.64898/2026.09.01.748461v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Full-length 16S profiling reveals individualized gut microbiota dynamics during short-duration spaceflight
AU - G. Gulyas
AU - B. Kakuk
AU - A. Dormo
AU - I. Prazsak
AU - T. Jaray
AU - Z. Csabai
AU - A. T. Schlegl
AU - Z. Boldogkoi
AU - D. Tombacz
PY - 2026
DA - 2026/09/01
PB - bioRxiv
DO - 10.64898/2026.09.01.748461
UR - https://www.biorxiv.org/content/10.64898/2026.09.01.748461v1
AB - Human spaceflight may perturb the gut microbiota, but densely sampled short missions remain poorly characterized. We profiled 27 phase-matched fecal samples from two astronauts during an 18-day International Space Station mission and one ground-based participant following the same daily schedule using Oxford Nanopore full-length 16S sequencing. Participant identity dominated genus-level Bray-Curtis variation (R2 = 0.489, p < 0.001). In astronaut-only community analyses, mission phase explained 23.4% of genus-level (p = 0.035) and 22.3% of species-level (p = 0.021) variation. Astronauts showed greater displacement from personal baselines than B1 (0.331 versus 0.171) and 1.58-fold higher volatility. Astronaut-only taxon models identified 2 of 81 genera and 5 of 139 species; Collinsella increased from quarantine to orbit (coefficient = 2.586, q = 0.037). Thus, the short-duration spaceflight interval was accompanied by individualized, temporally localized community and taxon shifts rather than uniform microbiota restructuring.
ER -