Skip to content
DraconiticResearch
Search Sources API
190,851 studies31 sourcesUpdated 40 min ago
Peer-reviewedCC BY 4.0Biomolecules

Distinct sFOLR1 and Cytokine Secretion Profiles Across Marker-Enriched OVCAR-3 Ovarian Cancer Cell Subpopulations: A Pilot Study

  • Andrei Pașca1,2
  • Eva Fischer-Fodor3
  • Paul Milan Kubelac4
  • Ovidiu Bălăcescu5
  • Oana Baldasici5
  • Bogdan Pop6
  • Natalia Miklášová7
  • Nicoleta-Monica Pașca4
  • Cătălin Ioan Vlad1,2
  • Patriciu Andrei Achimaș-Cadariu1,2
7 affiliations
  1. 1Department of Surgical Oncology and Gynecologic Oncology, ‘Iuliu Hațieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
  2. 2Department of Surgical Oncology, “Prof. Dr. Ion Chiricuta” Institute of Oncology, 400015 Cluj-Napoca, Romania
  3. 3Tumor Biology Department, “Prof. Dr. Ion Chiricuta” Institute of Oncology, 400015 Cluj-Napoca, Romania
  4. 4Department of Medical Oncology, “Prof. Dr. Ion Chiricuta” Institute of Oncology, 400015 Cluj-Napoca, Romania
  5. 5Department of Functional Genomics, Proteomics and Experimental Pathology, “Prof. Dr. Ion Chiricuta” Institute of Oncology, 400015 Cluj-Napoca, Romania
  6. 6Department of Pathology, “Prof. Dr. Ion Chiricuta” Institute of Oncology, 400015 Cluj-Napoca, Romania
  7. 7Department of Inorganic Chemistry, Faculty of Natural Sciences, Comenius University Bratislava, Ilkovičova 6, Mlynská dolina, 84215 Bratislava, Slovakia
Read full textPDFDOICite

Abstract

Background: Folate Receptor Alpha (FRα), encoded by the Folate Receptor 1 (FOLR1) gene, is expressed in many high-grade serous ovarian carcinomas, but the tumor-cell phenotypes contributing to soluble FOLR1 (sFOLR1) release remain unclear. This pilot study compared sFOLR1 and cytokine secretion across OVCAR-3 fractions enriched for CD133, EpCAM/CD326, CD90, and ABCG2/CD338. Methods: The parental OVCAR-3 culture was phenotyped by flow cytometry and separated into marker-positive and marker-negative fractions using CD133, EpCAM/CD326, and ABCG2/CD338 immunomagnetic selection. CD90-positive and corresponding negative fractions were isolated after indirect co-culture of OVCAR-3 cells with primary mesenchymal stromal cells. Paired fractions were cultured for 120 h, and sFOLR1, vascular endothelial growth factor (VEGF), interleukin (IL)-6, and IL-10 were measured at 24 h intervals. Results: sFOLR1 release varied markedly by time and phenotype. CD133-positive and CD326-positive fractions showed time-dependent increases; CD90-positive maintained low, comparatively stable concentrations; and CD338-positive declined from a high early value, while the corresponding negative fraction increased. In CD133-positive and CD326-positive fractions, sFOLR1 tracked with VEGF and/or IL-6, whereas CD90-positive and CD338-positive fractions showed distinct, partly uncoupled cytokine patterns. Conclusions: These exploratory associations in a single cell line support further investigation of sFOLR1 and cytokine profiles.

Related

  1. CD4+ and CD8+ lymphocyte responses and cytokine profiles in visceral leishmaniasis patients following effective treatment in distinct transmission settings in Bihar, India
    Rahul Tiwari, Siddharth Sankar Singh, Sarita Singh +6
    Frontiers in ImmunologyPeer-reviewedCC BY 4.0Full textEpidemiology & public healthCrossrefEurope PMCPMC
  2. Cytokine expression and secretion by skeletal muscle cells: regulatory mechanisms and exercise effects
    Jonathan M. Peake, Paul A. Della Gatta, Katsuhiko Suzuki +1
    PubMedPeer-reviewedLicence not statedFull text376 citedGeneral medicineOpenAlex
  3. Human rhinovirus 16 impairs macrophage cytokine secretion by disrupting NF-κB nuclear translocation and intracellular cytokine trafficking
    Zoé Fremont-Debaene, Zeyni Mansuroglu, Léane Puchot +5
    HALPreprintCC BY-NC-ND 4.0Full textOncologyHAL
  4. Influence of Occupational Exposures on Cytokine Profiles in Patients with Membranous Nephropathy
    Maxime Teisseyre, Mounir El Mai, Marion Cremoni +1
    Kidney international reportsPeer-reviewedCC BYFull textGeneral medicineEurope PMCEurope PMCPMC
  5. Cytokine Profiles in Cervical Intraepithelial Neoplasia: Associations with Lesion Severity and Exploratory Treatment Response
    Attila Louis Major, Aleksandra Riger, Nataliy Kharchenko +5
    Diagnostics (Basel, Switzerland)Peer-reviewedCC BYFull textGeneral medicineEurope PMC

Cite

BibTeXRIS
BibTeX
@article{pașca2026distinct,
  title = {{Distinct sFOLR1 and Cytokine Secretion Profiles Across Marker-Enriched OVCAR-3 Ovarian Cancer Cell Subpopulations: A Pilot Study}},
  author = {Andrei Pașca and Eva Fischer-Fodor and Paul Milan Kubelac and Ovidiu Bălăcescu and Oana Baldasici and Bogdan Pop and Natalia Miklášová and Nicoleta-Monica Pașca and Cătălin Ioan Vlad and Patriciu Andrei Achimaș-Cadariu},
  journal = {Biomolecules},
  year = {2026},
  month = oct,
  doi = {10.3390/biom16101462},
  url = {https://www.mdpi.com/2218-273X/16/10/1462},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Distinct sFOLR1 and Cytokine Secretion Profiles Across Marker-Enriched OVCAR-3 Ovarian Cancer Cell Subpopulations: A Pilot Study
AU  - Andrei Pașca
AU  - Eva Fischer-Fodor
AU  - Paul Milan Kubelac
AU  - Ovidiu Bălăcescu
AU  - Oana Baldasici
AU  - Bogdan Pop
AU  - Natalia Miklášová
AU  - Nicoleta-Monica Pașca
AU  - Cătălin Ioan Vlad
AU  - Patriciu Andrei Achimaș-Cadariu
PY  - 2026
DA  - 2026/10/01
JO  - Biomolecules
DO  - 10.3390/biom16101462
UR  - https://www.mdpi.com/2218-273X/16/10/1462
AB  - Background: Folate Receptor Alpha (FRα), encoded by the Folate Receptor 1 (FOLR1) gene, is expressed in many high-grade serous ovarian carcinomas, but the tumor-cell phenotypes contributing to soluble FOLR1 (sFOLR1) release remain unclear. This pilot study compared sFOLR1 and cytokine secretion across OVCAR-3 fractions enriched for CD133, EpCAM/CD326, CD90, and ABCG2/CD338. Methods: The parental OVCAR-3 culture was phenotyped by flow cytometry and separated into marker-positive and marker-negative fractions using CD133, EpCAM/CD326, and ABCG2/CD338 immunomagnetic selection. CD90-positive and corresponding negative fractions were isolated after indirect co-culture of OVCAR-3 cells with primary mesenchymal stromal cells. Paired fractions were cultured for 120 h, and sFOLR1, vascular endothelial growth factor (VEGF), interleukin (IL)-6, and IL-10 were measured at 24 h intervals. Results: sFOLR1 release varied markedly by time and phenotype. CD133-positive and CD326-positive fractions showed time-dependent increases; CD90-positive maintained low, comparatively stable concentrations; and CD338-positive declined from a high early value, while the corresponding negative fraction increased. In CD133-positive and CD326-positive fractions, sFOLR1 tracked with VEGF and/or IL-6, whereas CD90-positive and CD338-positive fractions showed distinct, partly uncoupled cytokine patterns. Conclusions: These exploratory associations in a single cell line support further investigation of sFOLR1 and cytokine profiles.
SN  - 2218-273X
ER  -