Immobilization of Aspergillus oryzae IPT-301 Cells with High Transfructosylation Activity on Polyurethane Sponge for Fructooligosaccharide Production in Batch Mode
5 affiliations
- 1Federal University of Alfenas , , , ,
- 2University of Minho , , Campus de Gualtar , ,
- 3Institute for Technological Research , , , ,
- 4São Paulo State University , , , ,
- 5Federal University of Tocantins , , , ,
Abstract
A stable and active biocatalyst for fructooligosaccharide (FOS) production was synthesized by immobilizing A. oryzae IPT-301 cells on a polyurethane sponge (PS) during submerged cultivation. Comparisons between immobilized and cross-linked immobilized cells revealed similar biochemical properties. The highest cell concentration immobilized in PS (11.89 ± 2.29 g cells g support–1) was obtained after 32 h. Both biocatalysts performed optimally at 50 °C, pH 5.5, and 480.2 g L–1 sucrose, showing a Vmax of 3500 μmol g–1 min–1 and high selectivity for 1-kestose (28.23%). They exhibited a half-life of 300 min at 50 °C, retained 40% activity after 28 days of storage and 50% activity after 12 reaction cycles. FOS productivity reached 116 g L–1 h–1 with a 24% yield. Statistical analysis confirmed that cross-linking did not significantly influence performance. The results show that in situ immobilization on PS is a simple, efficient strategy for producing stable biocatalysts for FOS synthesis.
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BibTeX
@article{barbosa2026immobilization,
title = {{Immobilization of Aspergillus oryzae IPT-301 Cells with High Transfructosylation Activity on Polyurethane Sponge for Fructooligosaccharide Production in Batch Mode}},
author = {Nathália Romani Barbosa and Ana Vieira and Fábio Aurélyo Silva Ferreira and Luara Cristina Alves and Sara Silverio and Sylma Carvalho Maestrelli and Sérgio Fernandes and Alfredo Eduardo Maiorano and Elda Sabino da Silva and Cristiane Ottoni and Sergio Villalba Morales and Rafael Firmani Perna and Michelle Xavier},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c07568},
url = {https://pubs.acs.org/acsodf/article/11/42/65224/5450576/Immobilization-of-Aspergillus-oryzae-IPT-301-Cells},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Immobilization of Aspergillus oryzae IPT-301 Cells with High Transfructosylation Activity on Polyurethane Sponge for Fructooligosaccharide Production in Batch Mode
AU - Nathália Romani Barbosa
AU - Ana Vieira
AU - Fábio Aurélyo Silva Ferreira
AU - Luara Cristina Alves
AU - Sara Silverio
AU - Sylma Carvalho Maestrelli
AU - Sérgio Fernandes
AU - Alfredo Eduardo Maiorano
AU - Elda Sabino da Silva
AU - Cristiane Ottoni
AU - Sergio Villalba Morales
AU - Rafael Firmani Perna
AU - Michelle Xavier
PY - 2026
DA - 2026/10/14
JO - ACS Omega
DO - 10.1021/acsomega.6c07568
UR - https://pubs.acs.org/acsodf/article/11/42/65224/5450576/Immobilization-of-Aspergillus-oryzae-IPT-301-Cells
AB - A stable and active biocatalyst for fructooligosaccharide (FOS) production was synthesized by immobilizing A. oryzae IPT-301 cells on a polyurethane sponge (PS) during submerged cultivation. Comparisons between immobilized and cross-linked immobilized cells revealed similar biochemical properties. The highest cell concentration immobilized in PS (11.89 ± 2.29 g cells g support–1) was obtained after 32 h. Both biocatalysts performed optimally at 50 °C, pH 5.5, and 480.2 g L–1 sucrose, showing a Vmax of 3500 μmol g–1 min–1 and high selectivity for 1-kestose (28.23%). They exhibited a half-life of 300 min at 50 °C, retained 40% activity after 28 days of storage and 50% activity after 12 reaction cycles. FOS productivity reached 116 g L–1 h–1 with a 24% yield. Statistical analysis confirmed that cross-linking did not significantly influence performance. The results show that in situ immobilization on PS is a simple, efficient strategy for producing stable biocatalysts for FOS synthesis.
SN - 2470-1343
ER -