Differential formation of nitrate-responsive mycobacterial intracytoplasmic membranes during Mycobacterium tuberculosis infection
Abstract
Subcellular compartmentalization of metabolic processes is a main feature of prokaryotic and eukaryotic architecture. Environmental bacteria generate intracytoplasmic membranes (ICMs) as a crucial strategy to adapt their metabolism to environmental changes. While pathogenic intracellular bacteria also perceive various stressful stimuli during host interactions, the subsequent re-organization of their internal architecture has not been explored. Using cryo-electron tomography, we show that Mycobacterium tuberculosis (Mtb), a major human pathogen, is able to form ICMs outside and inside host cells in a strain-dependent manner. We characterize these Mycobacterial intracytoplasmic Membranes (MIMs) as nitrate-induced structures. Furthermore, we uncover that MIM formation in Mtb during macrophage infection correlates with limited cellular inflammatory responses. Our findings reveal a cytoplasmic structure in Mtb and link it to a functional mechanism that enables the bacterium to adapt to its intracellular niche.
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BibTeX
@article{keck2026differential,
title = {{Differential formation of nitrate-responsive mycobacterial intracytoplasmic membranes during Mycobacterium tuberculosis infection}},
author = {Camille Keck and Stéphane Tachon and Fadel Sayes and Daniele Musiani and Quentin Giai Gianetto and Mariette Matondo and Matthijn Vos and Roland Brosch and Jost Enninga},
journal = {Cell Reports},
year = {2026},
month = oct,
doi = {10.1016/j.celrep.2026.118078},
url = {https://pasteur.hal.science/pasteur-05781660v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Differential formation of nitrate-responsive mycobacterial intracytoplasmic membranes during Mycobacterium tuberculosis infection
AU - Camille Keck
AU - Stéphane Tachon
AU - Fadel Sayes
AU - Daniele Musiani
AU - Quentin Giai Gianetto
AU - Mariette Matondo
AU - Matthijn Vos
AU - Roland Brosch
AU - Jost Enninga
PY - 2026
DA - 2026/10/01
JO - Cell Reports
DO - 10.1016/j.celrep.2026.118078
UR - https://pasteur.hal.science/pasteur-05781660v1
AB - Subcellular compartmentalization of metabolic processes is a main feature of prokaryotic and eukaryotic architecture. Environmental bacteria generate intracytoplasmic membranes (ICMs) as a crucial strategy to adapt their metabolism to environmental changes. While pathogenic intracellular bacteria also perceive various stressful stimuli during host interactions, the subsequent re-organization of their internal architecture has not been explored. Using cryo-electron tomography, we show that Mycobacterium tuberculosis (Mtb), a major human pathogen, is able to form ICMs outside and inside host cells in a strain-dependent manner. We characterize these Mycobacterial intracytoplasmic Membranes (MIMs) as nitrate-induced structures. Furthermore, we uncover that MIM formation in Mtb during macrophage infection correlates with limited cellular inflammatory responses. Our findings reveal a cytoplasmic structure in Mtb and link it to a functional mechanism that enables the bacterium to adapt to its intracellular niche.
SN - 2211-1247
SN - 2211-1247
ER -