Mechanical forces stimulate Golgi export
Abstract
Cells face diverse mechanical stimuli that vary with cell type, state, and pathological conditions. Mechanobiology investigates how cells sense and respond to these forces. While most work has focused on the cell surface and nucleus as primary mechanosensors, how intracellular organelles adapt to extracellular mechanical forces remains largely unknown. Here, we show that extracellular mechanical signals influence the secretory function of the Golgi apparatus. By subjecting adherent cells to mechanical challenges—cell spreading on different ligands, altered substrate stiffness, or equibiaxial strain—we reveal that extracellular forces modulate Golgi-to-cell surface carrier biogenesis, thereby regulating exocytosis. Together with changes in Golgi membrane tension, we identify molecular determinants of the mechanotransduction pathway, including microtubule acetylation, diacylglycerol production, and protein kinase D activity. In turn, inhibition of Golgi export suppresses this mechanoresponse and causes impaired cell spreading. These findings uncover a bidirectional mechanotransduction axis in which extracellular mechanics tune Golgi secretory output, providing a framework for investigating organelle-based mechanoadaptation in physiology and disease.
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BibTeX
@article{naidu2026mechanical,
title = {{Mechanical forces stimulate Golgi export}},
author = {Chandini Bhaskar Naidu and Javier Vera Lillo and Eugenia Almacellas and Anabel-Lise Le Roux and Sabine Bardin and Nicolas Mateos and Jessica Angulo-Capel and Adam Wolowczyk and Yugo Terashima and Yuichi Wakana and Pere Roca-Cusachs and Maria F Garcia-Parajo and Franck Perez and Bruno Goud and Jean-Baptiste Manneville and Stéphanie Miserey and Felix Campelo},
journal = {Journal of Cell Biology},
year = {2026},
month = oct,
doi = {10.1083/jcb.202510026},
url = {https://hal.science/hal-05780833v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Mechanical forces stimulate Golgi export
AU - Chandini Bhaskar Naidu
AU - Javier Vera Lillo
AU - Eugenia Almacellas
AU - Anabel-Lise Le Roux
AU - Sabine Bardin
AU - Nicolas Mateos
AU - Jessica Angulo-Capel
AU - Adam Wolowczyk
AU - Yugo Terashima
AU - Yuichi Wakana
AU - Pere Roca-Cusachs
AU - Maria F Garcia-Parajo
AU - Franck Perez
AU - Bruno Goud
AU - Jean-Baptiste Manneville
AU - Stéphanie Miserey
AU - Felix Campelo
PY - 2026
DA - 2026/10/05
JO - Journal of Cell Biology
DO - 10.1083/jcb.202510026
UR - https://hal.science/hal-05780833v1
AB - Cells face diverse mechanical stimuli that vary with cell type, state, and pathological conditions. Mechanobiology investigates how cells sense and respond to these forces. While most work has focused on the cell surface and nucleus as primary mechanosensors, how intracellular organelles adapt to extracellular mechanical forces remains largely unknown. Here, we show that extracellular mechanical signals influence the secretory function of the Golgi apparatus. By subjecting adherent cells to mechanical challenges—cell spreading on different ligands, altered substrate stiffness, or equibiaxial strain—we reveal that extracellular forces modulate Golgi-to-cell surface carrier biogenesis, thereby regulating exocytosis. Together with changes in Golgi membrane tension, we identify molecular determinants of the mechanotransduction pathway, including microtubule acetylation, diacylglycerol production, and protein kinase D activity. In turn, inhibition of Golgi export suppresses this mechanoresponse and causes impaired cell spreading. These findings uncover a bidirectional mechanotransduction axis in which extracellular mechanics tune Golgi secretory output, providing a framework for investigating organelle-based mechanoadaptation in physiology and disease.
SN - 0021-9525
SN - 1540-8140
ER -