Cell-type specific transcriptional risk scores and longitudinal multiple sclerosis outcomes
- 1University of Nottingham
Abstract
Transcriptional risk scores, an integrated score of genomic susceptibility variants with expression Quantitative Trait Loci (eQTL) data, may more accurately distinguish clinical phenotypes compared to polygenic risk scores. This approach has not been applied to multiple sclerosis. We integrated data from the largest GWAS of MS, involving 14,802 MS patients, with eQTL data from 7,466 blood donors and 5,494 brain donors, using summary-data-based Mendelian randomization (SMR) followed by the heterogeneity in dependent instruments (HEIDI) test. MS SNVs with a significant eQTL were integrated with single-cell RNA sequencing data from 1,27 million peripheral blood mononuclear cells from 927 donors and 750,614 single nucleus RNA sequencing central nervous system cells from 192 individuals using Bayesian colocalization. Derived polygenic and cell-line specific transcriptional risk scores were compared with age-related MS severity score (ARMSS) in a cohort of 1,077 MS patients from the South Wales MS Registry and with MRI-derived age-adjusted total brain volumes of 272 MS patients within the UK Biobank. Cell-line specific transcriptional risk scores were associated with MS outcomes in two independent cohorts. Our results identified MS-associated eGenes enriched across several biological pathways, providing insights into pathophysiological mechanisms in MS that could facilitate precision medicine and identify novel druggable pathways.
Related
- A longitudinal analysis of real world symptom tracking and patient-reported outcomes in Brisa, a German Digital Health App for multiple sclerosis
- Association Between Local Ascending Aortic Elastic Properties and Left Ventricular Global Longitudinal Strain in Patients with Multiple Sclerosis: A Cross-Sectional Case–Control Echocardiographic Study
- Domain-Specific Cognitive Associations With Standing Balance in Multiple Sclerosis
- Gluten-Free and Gluten-Restricted Diets in Multiple Sclerosis: A Systematic Review of Disease Activity and Patient-Reported Outcomes
- Baseline miR-10a, miR-146a, and miR-155 expression and subsequent clinical and radiological outcomes in multiple sclerosis : A retrospective observational study
BibTeX
@misc{upcott2026celltype,
title = {{Cell-type specific transcriptional risk scores and longitudinal multiple sclerosis outcomes}},
author = {M. Upcott and B. Shao and N. J. Bray and S. Loveless and E. C. Tallantyre and N. P. Robertson and K. L. Kreft},
howpublished = {medRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.21.26363545},
url = {https://www.medrxiv.org/content/10.64898/2026.09.21.26363545v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Cell-type specific transcriptional risk scores and longitudinal multiple sclerosis outcomes
AU - M. Upcott
AU - B. Shao
AU - N. J. Bray
AU - S. Loveless
AU - E. C. Tallantyre
AU - N. P. Robertson
AU - K. L. Kreft
PY - 2026
DA - 2026/09/21
PB - medRxiv
DO - 10.64898/2026.09.21.26363545
UR - https://www.medrxiv.org/content/10.64898/2026.09.21.26363545v1
AB - Transcriptional risk scores, an integrated score of genomic susceptibility variants with expression Quantitative Trait Loci (eQTL) data, may more accurately distinguish clinical phenotypes compared to polygenic risk scores. This approach has not been applied to multiple sclerosis. We integrated data from the largest GWAS of MS, involving 14,802 MS patients, with eQTL data from 7,466 blood donors and 5,494 brain donors, using summary-data-based Mendelian randomization (SMR) followed by the heterogeneity in dependent instruments (HEIDI) test. MS SNVs with a significant eQTL were integrated with single-cell RNA sequencing data from 1,27 million peripheral blood mononuclear cells from 927 donors and 750,614 single nucleus RNA sequencing central nervous system cells from 192 individuals using Bayesian colocalization. Derived polygenic and cell-line specific transcriptional risk scores were compared with age-related MS severity score (ARMSS) in a cohort of 1,077 MS patients from the South Wales MS Registry and with MRI-derived age-adjusted total brain volumes of 272 MS patients within the UK Biobank. Cell-line specific transcriptional risk scores were associated with MS outcomes in two independent cohorts. Our results identified MS-associated eGenes enriched across several biological pathways, providing insights into pathophysiological mechanisms in MS that could facilitate precision medicine and identify novel druggable pathways.
ER -